Registration Dossier
Registration Dossier
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EC number: 235-835-1 | CAS number: 13001-39-3
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- August 1977
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 978
Materials and methods
- Principles of method if other than guideline:
- According to HLE protocol 543/1.
- GLP compliance:
- no
- Test type:
- standard acute method
- Limit test:
- yes
Test material
- Reference substance name:
- 2,2'-(p-phenylenediethene-2,1-diyl)bisbenzonitrile
- EC Number:
- 235-835-1
- EC Name:
- 2,2'-(p-phenylenediethene-2,1-diyl)bisbenzonitrile
- Cas Number:
- 13001-39-3
- Molecular formula:
- C24H16N2
- IUPAC Name:
- 2,2'-(p-phenylenediethene-2,1-diyl)bisbenzonitrile
- Test material form:
- solid: particulate/powder
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Bantin and Kingman Ltd. Grimston, Aldbrough, Nr. Hull
- Weight at study initiation: 200 - 300 g
- Fasting period before study: 18-20 h(overnight)
- Housing: single air-conditioned room; caged in groups of 5 by sex in suspended grid floor cages
- Diet: ad libitum
- Water: ad libitum
- Acclimation period: minimum 3 days
ENVIRONMENTAL CONDITIONS
- Temperature: 22 ± 3 °C
- Humidity: 50 ± 10 %
- Photoperiod: natural lighting conditions
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- water
- Doses:
- 2000 mg/kg
- No. of animals per sex per dose:
- 5 sex/dose
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: on day 7 and 14 after treatment
- Necropsy of survivors performed: no
- Other examinations performed: clinical bobservations, body weights
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 2 000 mg/kg bw
- Based on:
- test mat.
- Mortality:
- No deaths.
- Clinical signs:
- other: other: other: other: No signs of overt toxicity.
Any other information on results incl. tables
Group mean body weights in g
| dose level (mg/kg) | no. of animal/sex | group mean body weights | ||
| day before treatment | day 7 | day 14 | ||
| 2000 | 5 F | 254 | 282 | 315 |
| 2000 | 5 M | 224 | 247 | 261 |
All animals survived until the end of the observation period.
Applicant's summary and conclusion
- Interpretation of results:
- other: not classified according to the CLP Regulation (EC 1272/2008)
- Conclusions:
- LD50 (rat) > 2000 mg/kg bw.
- Executive summary:
Method
Acute toxicity study by oral route, carried out as limit test. A dose of 2000 mg/kg bw was adminstered by gavage to 5 rat/sex, using distilled water as vehicle; animals were observed up to 14 days after dosing.
Results
No deaths occurred, thus an LD50 > 2000 mg/kg was established. Body weight gains during the study period were normal.
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