4-ethyl-2,3-dioxopiperazine-1-carbonyl chloride

Endpoint summary

Description of key information

Short-term toxicity to fish:

Based on the EPI Suite ECOSAR version 1.10 predicted model, the 96 hours LC50 was estimated to be 222000 mg/l on Fish for substance 4-ethyl-2,3-dioxopiperazine-1-carbonyl chloride (Cas no. 59703-00-3) on the basis of mortality effects.

Toxicity to aquatic algae and cyanobacteria

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the five closest read across substances, the short term toxicity on aquatic algae and cyanobacteria was predicted for target substance 4-ethyl-2,3-dioxopiperazine-1-carbonyl chloride (CAS no. 59703 -00 -3) (2017). EC50 value was estimated to be 260 mg/l for Pseudokirchneriella subcapitata for 72 h duration. Based on this value it can be concluded that the substance 4-ethyl-2,3-dioxopiperazine-1-carbonyl chloride is considered to be non-toxic to aquatic environment and can be considered to be not classified as per the CLP classification criteria.

Short term toxicity to aquatic invertebrates

The prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the five closest read across substances, the short term toxicity on aquatic invertebrates was predicted for 4-ethyl-2,3-dioxopiperazine-1-carbonyl chloride (CAS no 59703-00-3). EC50 value was estimated to be 478.57 mg/L on the basis of intoxication for Daphnia magna for 48 hrs duration.

Hence, it can be considered that 4-ethyl-2,3-dioxopiperazine-1-carbonyl chloride (59703-00-3)

was likely to be non-toxic to aquatic invertebrates at environmentally relevant concentrations and can be considered to be not classified as per the criteria of CLP regulation.

Additional information

Short-term toxicity to fish:

Based on the EPI Suite ECOSAR version 1.10 predicted model, the 96 hours LC50 was estimated to be 222000 mg/l on Fish for substance 4-ethyl-2,3-dioxopiperazine-1-carbonyl chloride (Cas no. 59703-00-3) on the basis of mortality effects using neutral organics class for prediction.Based on this value it can be concluded that the substance 4-ethyl-2,3-dioxopiperazine-1-carbonyl chloride is considered to be non-toxic to aquatic environment and can be considered to be not classified as per the CLP classification criteria.

Short term toxicity to aquatic invertebrates

Short term toxicity to aquatic invertebrates was reviewed for 4-ethyl-2,3-dioxopiperazine-1-carbonyl chloride (59703-00-3) with two predicted data and two experimental data for functionally similar read across Dimethylformamide (68-12-2) and Tetraacetylethylenediamine (10543-57-4) are as follows:

In the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the five closest read across substances, the short term toxicity on aquatic invertebrates was predicted for 4-ethyl-2,3-dioxopiperazine-1-carbonyl chloride (CAS no 59703-00-3). EC50 value was estimated to be 478.57 mg/L on the basis of intoxication for Daphnia magna for 48 hrs duration.

Another prediction on short-term toxicity of the substance to invertebrate was predicted using EPI suite, ECOSAR version 1.1, 2017, based on the effects observed in 48 hr exposure. The lethal concentration (LC50) for the 4-ethyl-2,3-dioxopiperazine-1-carbonyl chloride (59703-00-3) was estimated to be 91580.016 mg/l.

Study was performed in read across Dimethylformamide (68-12-2) by Sebaughet. al, 1991 in Daphnia magna for 48 hrs in static test conditions. Less than 24 hrs daphnids were used for treatment from adults. The 250 ml beaker was used with 200 ml of test concentrations. Well water was the dilution water used for the test and test solution was not aerated during test. Test was conducted with 12 test concentrations and for test pH, dissolved oxygen, alkalinity, and hardness were monitor at the beginning and end of the test. Probit and logit model are the two parameter models which assume symmetry of response. After the experiment, EC 50 value for Dimethylformamide (68-12-2) was determined to be 13700 mg/l.

Another study on read across tetraacetylethylenediamine (10543-57-4) was performed in Daphnia magna for 24 and 48 hrs. After the period, the EC 50 value of Tetraacetylethylenediamine (10543-57-4) was determined to be >500 and >800 mg/l for 24 and 48 hrs.

Thus, based on the predicted results 4-ethyl-2,3-dioxopiperazine-1-carbonyl chloride (59703-00-3) and study from read across, it can be considered that all the above weight of evidences has value greater than 100 mg/l. Hence, it can be considered that 4-ethyl-2,3-dioxopiperazine-1-carbonyl chloride (59703-00-3) was likely to be non-toxic to aquatic invertebrates at environmentally relevant concentrations and can be considered to be not classified as per the criteria of CLP regulation.

Toxicity to aquatic algae and cyanobacteria

2 predicted datafor the target chemical4-ethyl-2,3-dioxopiperazine-1-carbonyl chloride (CAS no. 59703-00-3) which is supported further by total 2 weight of evidence studies (from secondary source) for its closest read across substance with logKow as the primary descriptor were reviewed for the toxicity to aquatic algae and cyanobacteria end point which are summarized as below:

 

Short term toxicity on aquatic algae and cyanobacteria oftarget chemical4-ethyl-2,3-dioxopiperazine-1-carbonyl chloride (CAS no. 59703-00-3), is predicted using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the five closest read across substances (2017). EC50 value was estimated to be 260 mg/l for Pseudokirchneriella subcapitata for 72 h duration. Based on this value it can be concluded that the substance 4-ethyl-2,3-dioxopiperazine-1-carbonyl chloride is considered to be non-toxic to aquatic environment and can be considered to be not classified as per the CLP classification criteria.

 

In another prediction done using the EPI Suite ECOSAR version 1.10 (2017), short term toxicity on green algae was predicted for test substance 4 -ethyl-2,3 -dioxopiperazine-1 -carbonyl chloride (CAS: 59703 -00 -3). On the basis of effects observed in a static freshwater system, the effect concentration EC50 value for the substance is estimated to be 18098.6 mg/l for green algae for 96 hr duration. Based on this value, it can be concluded that the test chemical 4 -ethyl-2,3 -dioxopiperazine-1 -carbonyl chloride can be considered as non-toxic to green algae at environmentally relevant concentrations and can be considered to be not classified as per the CLP classification criteria.

 

In a supporting weight of evidence study of read across chemical 2 -(2 -Dimethylaminoethoxy)ethanol (CAS no. 1704-62-7) from OECD SIDS (2014), short term toxicity to Pseudokirchnerella subcapitata (algae) study was carried out for 72 hrs. The study was based on the effects of the test compound 2 -(2 -Dimethylaminoethoxy)ethanol on Pseudokirchnerella subcapitata in a static fresh water system. The 72hr-EC50 and NOEC values of 2 -(2 -Dimethylaminoethoxy)ethanol to Pseudokirchnerella subcapitata was determined as 160 and 40 mg/L for growth rate and 72 hr EC50 value was determined to be 73 mg/L for area under growth curve, respectively. Thus, based on EC50 value (160 mg/l), it can be concluded that the substance 2 -(2 -Dimethylaminoethoxy)ethanol can be considered as non-toxic to aquatic organisms and thus cannot be classified as hazardous as per the CLP classification criteria.

 

Another short term toxicity study to Selenastrum capricornutum (algae) of read across substance N-phenylacetamide (CAS no. 103 -84 -4) was carried out for 72 hrs (OECD SIDS, 2001). The study was performed according to OECD TG 201 Guideline, “Alga, Growth Inhibition Test”. The study was based on the effects of the test compound N-phenylacetamide on Selenastrum capricornutum in a static fresh water system at a temperature of 22 -24°C. Stock solution was prepared with OECD medium and sterilized through 0.45 μm acrodisc filter. Test chemical conc. used for the study were 3, 6, 13, 25, 50, 100 mg/l (nominal conc.) and 4, 8, 18, 29, 58, 105 mg/l (measured conc.), respectively.Selenastrum capricornutum ATCC 22662 was used as test organism obtained from American Type Culture Collection and subcutured at the testing facility. Test was carried out in an100 ml Erlenmeyer flask at a temperature range of 22 -24°C and light intensity of 8,220 - 8,312 Lux, continuous, respectively. Standard OECD medium was used as growth medium. Non-linear regression analysis was employed to determine EC50 by Comprehensive Toxicity Data Analysis and Database Software (Version 5.0). For the determination of NOEC, Dennett’s test was used (p < 0.05). Cell density in control increased by 120 times at the termination of experiment (72 hr). Based on the effect on biomass of test organismSelenastrum capricornutum, the 72 hr EC50 and NOEC value for chemical N-phenylacetamide was determined to be 13.5 and < 4 mg/l, respectively. Thus, based on EC50 value, it can be concluded that the substance N-phenylacetamide can be considered as toxic to aquatic organisms. Since the chemical is readily biodegradable in nature, chemical N-phenylacetamide can be considered as non-toxic to aquatic organisms and thus cannot be classified as hazardous as per the CLP classification criteria.

 

Thus, based on the overall reported results for target chemical 4-ethyl-2,3-dioxopiperazine-1-carbonyl chloride (from OECD QSAR toolbox 3.3 and EPI suite, 2017) and for its read across substance (from secondary source OECD SIDS), it can be concluded that the test substance 4-ethyl-2,3-dioxopiperazine-1-carbonyl chloride can be considered as non-toxic to aquatic environment and can be considered to be not classified as per the CLP classification criteria.