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EC number: 202-068-9 | CAS number: 91-44-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Justification for type of information:
- Data is from peer reviewed publication
Data source
Reference
- Reference Type:
- publication
- Title:
- Gene mutaton toxicity study of the test chemical
- Author:
- Haworth et al
- Year:
- 1 983
- Bibliographic source:
- Environmental Mutagenesis
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Principles of method if other than guideline:
- The Salmonella/mammalian microsome test was performed to determine the mutagenic nature of the test chemical in vitro
- GLP compliance:
- not specified
- Type of assay:
- bacterial gene mutation assay
Test material
- Reference substance name:
- 7-(diethylamino)-4-methyl-2-benzopyrone
- EC Number:
- 202-068-9
- EC Name:
- 7-(diethylamino)-4-methyl-2-benzopyrone
- Cas Number:
- 91-44-1
- Molecular formula:
- C14H17NO2
- IUPAC Name:
- 7-(diethylamino)-4-methyl-2H-chromen-2-one
- Details on test material:
- - Name of test material: 7-Diethylamino-4-methylcoumarin
- Molecular formula: C14H17NO2
- Molecular weight: 231.2933 g/mol
- Substance type: Organic
- Physical state: Powder Solid
- Purity: Label purity: Practical
Analyzed purity: No data available
- Impurities (identity and concentrations): No data available
Constituent 1
Method
- Target gene:
- Histidine
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Details on mammalian cell type (if applicable):
- Not applicable
- Additional strain / cell type characteristics:
- not specified
- Cytokinesis block (if used):
- No data
- Metabolic activation:
- with and without
- Metabolic activation system:
- Liver S-9 fractions were prepared from male Sprague-Dawley rats and male Syrian hamsters
- Test concentrations with justification for top dose:
- 0, 100, 333, 1000, 3333, 5450 µg/plate
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO
- Justification for choice of solvent/vehicle: Soluble and stable in DMSO
Controls
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- sodium azide
- other: 2-Aminoanthracene, 4-Nitro-o-phenylenediamine
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: Preincubation
DURATION
- Preincubation period: 20 mins
- Exposure duration: 48 h
- Expression time (cells in growth medium): 48 h
- Selection time (if incubation with a selection agent): No data available
- Fixation time (start of exposure up to fixation or harvest of cells): No data available
SELECTION AGENT (mutation assays): No data available
SPINDLE INHIBITOR (cytogenetic assays): No data available
STAIN (for cytogenetic assays): No data available
NUMBER OF REPLICATIONS: Three plates were used, and the experiment was repeated no less than 1 week after completion of the initial test.
NUMBER OF CELLS EVALUATED: No data available
DETERMINATION OF CYTOTOXICITY
- Method: mitotic index; cloning efficiency; relative total growth; other: No data available
OTHER EXAMINATIONS:
- Determination of polyploidy: No data available
- Determination of endoreplication: No data available
- Other: No data available
OTHER: No data available - Rationale for test conditions:
- No data
- Evaluation criteria:
- Increase in the number of revertants
- Statistics:
- Statistical analysis of Salmonella plate test data was performed as per Margolin et al, 1981
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not specified
- True negative controls validity:
- not specified
- Positive controls validity:
- valid
- Additional information on results:
- TEST-SPECIFIC CONFOUNDING FACTORS
- Effects of pH: No data available
- Effects of osmolality: No data available
- Evaporation from medium: No data available
- Water solubility: No data available
- Precipitation: No data available
- Other confounding effects: No data available
RANGE-FINDING/SCREENING STUDIES: To select the dose range for the mutagenesis assay, the test chemicals were checked for toxicity to TA100 up to a concentration of 10 mg/plate or the limit of solubility, both in the presence and absence of S-9 mix. One or more parameters were used as an indication of toxicity: viability on complete medium (EGG) and reduced numbers of revertant colonies per plate and/or thinning or absence of the bacterial lawn (CWR, EGG, SRI). If toxicity was not apparent in the preliminary toxicity determination, the highest dose tested was 10 mg/plate; otherwise the upper limit of solubility was used. If toxicity was observed, the doses of test chemical were chosen so that the high dose exhibited some degree of toxicity.
COMPARISON WITH HISTORICAL CONTROL DATA: No data available - Remarks on result:
- other: No mutagenic potential
Any other information on results incl. tables
Dose(µg/plate) |
TA100 |
||
NA |
RLI |
HLI |
|
0.0(Solvent control) |
114±10.5 |
151±13.9 |
132±5.2 |
100.0 |
118±5.9 |
148±15.2 |
115±6.1 |
333.0 |
108±3.3 |
121±8.8 |
113±2.3 |
1000.0 |
93±3.5 |
107±2.5 |
130±4.6 |
3333.0 |
127±10.9 |
140±4.4 |
127±18.0 |
5450.0 |
122±12.3 |
141±9.6 |
150±21.5 |
POS |
506±21.0 |
698±47.9 |
1522±90.7 |
Dose(µg/plate) |
TA1535 |
||
NA |
RLI |
HLI |
|
0.0(Solvent control) |
6±2.2 |
12±0.6 |
5±1.2 |
100.0 |
7±1.2 |
8±1.9 |
6±1.9 |
333.0 |
4±0.3 |
7±0.6 |
7±2.0 |
1000.0 |
4±1.8 |
5±1.2 |
3±1.5 |
3333.0 |
2±1.5 |
7±1.3 |
3±1.5 |
5450.0 |
3±0.3 |
5±1.7 |
2±0.6 |
POS |
345±6.2 |
143±40.0 |
77±4.3 |
Dose(µg/plate) |
TA1537 |
||
NA |
RLI |
HLI |
|
0.0(Solvent control) |
4±1.5 |
8±0.0 |
10±1.5 |
100.0 |
5±0.9 |
6±0.6 |
11±3.2 |
333.0 |
5±1.0 |
11±2.6 |
7±1.5 |
1000.0 |
5±1.2 |
9±2.0 |
8±2.6 |
3333.0 |
3±0.7 |
9±1.9 |
8±3.2 |
5450.0 |
3±1.0 |
6±2.6 |
7±0.9 |
POS |
829±8.5 |
60±8.2 |
69±2.7 |
Dose(µg/plate) |
TA98 |
||
NA |
RLI |
HLI |
|
0.0(Solvent control) |
16±2.0 |
21±1.7 |
23±1.2 |
100.0 |
16±0.9 |
16±2.0 |
23±1.8 |
333.0 |
13±0.7 |
17±2.0 |
21±4.7 |
1000.0 |
15±2.0 |
22±5.9 |
18±3.8 |
3333.0 |
15±4.1 |
23±2.3 |
20±4.6 |
5450.0 |
9±1.2 |
20±2.7 |
22±3.4 |
POS |
348±29.0 |
414±29.4 |
1263±155.7 |
RLI: rat liver S-9 Aroclor 1254 induced;
HLI: hamster liver S-9Aroclor 1254 induced,
Mutagenic responses of Salmonella tester strains TA100, TA1535, TA1537, and TA98 (mean ± SEM) to test chemicals.
Applicant's summary and conclusion
- Conclusions:
- The test chemical is not mutagenic to Salmonella typhimurium TA100, TA1535, TA1537, TA98 in the preincubation assay performed with and without S9 metabolic activation system and hence does not classify as a gene mutant in vitro.
- Executive summary:
The Salmonella/mammalian microsome test was performed to determine the mutagenic nature of test chemical in vitro.
Preincubation assay was performed using Salmonella typhimurium TA100, TA1535, TA1537, TA98 with and without S9 metabolic activation system. To select the dose range for the mutagenesis assay, the test chemicals were checked for toxicity to TA100 up to a concentration of 10 mg/plate or the limit of solubility, both in the presence and absence of S-9 mix.
The doses thus selected were 0, 100, 333, 1000, 3333, 5450 µg/plate. Appropriate positive controls were also incorporated in the study.
The test chemical is not mutagenic to Salmonella typhimurium TA100, TA1535, TA1537, TA98 in the preincubation assay performed with and without S9 metabolic activation system and hence does not classify as a gene mutant in vitro.
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