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EC number: 244-096-4 | CAS number: 20882-04-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2016/01/14 - 2016/03/07
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: The study was performed following a standardised and suitable test guideline. The study was performed under GLP.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 016
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- [2-[(2-methyl-1-oxoallyl)oxy]ethyl] hydrogen succinate
- EC Number:
- 244-096-4
- EC Name:
- [2-[(2-methyl-1-oxoallyl)oxy]ethyl] hydrogen succinate
- Cas Number:
- 20882-04-6
- Molecular formula:
- C10H14O6
- IUPAC Name:
- 4-{2-[(2-methylprop-2-enoyl)oxy]ethoxy}-4-oxobutanoic acid
- Reference substance name:
- [SPEC][/SPEC][SYN][/SYN]
- IUPAC Name:
- [SPEC][/SPEC][SYN][/SYN]
- Reference substance name:
- [CS]000000111817[/CS]
- IUPAC Name:
- [CS]000000111817[/CS]
- Test material form:
- other: liquid
- Details on test material:
- Company internal substance number SAT 150073, Batch 4006/31
Colorless liquid at room temperature
Constituent 1
Constituent 2
Constituent 3
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Duplicate samples from the freshly prepared test media (containing algae) of all test concentrations and from the controls were taken at the start of the test.
For the determination of the stability of the test item under the test conditions and of the maintenance of the test item concentrations during the test period, duplicate samples from the test media of all test concentrations and the controls (containing algae) were taken at the end of the test (after the 72 hours test period) by pouring together the contents of the test beakers of each treatment.
The samples were diluted by a factor of two with acetonitrile.
Additional samples of the control blank and the dilution solvent were taken at test start and test end without any sample treatment.
The concentrations of the test item SAT 150073, [2-[(2-methyl-1-oxoallyl)oxy]ethyl] hydrogen succinate were analysed in the duplicate test media samples from all test concentrations, and in the duplicate control samples, from both sampling times (0 and 72 hours).
Test solutions
- Vehicle:
- no
- Details on test solutions:
- The test medium of the highest test concentration of nominal 315.5 mg test item/L was prepared by dissolving 319.5 mg test item into 1012.7 mL test water by intense stirring for 15 minutes. The pH of the stock solution was adjusted with 2 mol NaOH from 4.7 to 8.2. Adequate volumes of this test medium were diluted with test water to prepare the test media of the other desired test concentrations. The test media were prepared just before introduction of the algae (= start of the test).
Test organisms
- Test organisms (species):
- Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- Pseudokirchneriella subcapitata (KORSHIKOV), Strain No. 61.81 SAG, formerly known as Selenastrum capricornutum, and recently renamed as Raphidocelis subcapitata (KORSHIKOV)
The algae were supplied by the „Sammlung von Algenkulturen, Albrecht-von-Haller-Institut für Pflanzenwissenschaften, Universität Göttingen", 37073 Göttingen, Germany.
The algae were cultivated in the laboratories of ibacon under standardised conditions according to the test guidelines.
For the evaluation of the quality of the algae and the experimental conditions the reference item potassium dichromate is tested at least twice a year to demonstrate satisfactory test conditions.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Hardness:
- Calculated water hardness of the test water: 0.24 mmol/L (= 24 mg/L) as CaCO3.
- Test temperature:
- 22 - 23 °C
- pH:
- The pH was measured in all test item concentrations and the control at the start and the end of the test.
pH was: 8.1 to 8.2 at test start and 8.0 to 10.0 at test end - Nominal and measured concentrations:
- Mean Recovery in the Test Samples
Freshly prepared: 102% (n=10, RSD 1%)
Aged Test Media: 68% (n=10, RSD 32%)
Summary of Analytical Results
Sample Description % of nominal1 Geometric Mean Value2
fresh (0h) aged (72h)
[mg test item/L] [%] [%] [%] [mg test item/L]
Control n.a. n.a. n.a. n.a.
3.17 101 65 81 2.57
10 102 48 70 7.03
31.6 103 43 67 21.1
99.9 101 85 92 92.4
315.5 101 96 99 312
1 mean value of all measured samples per treatment group
2 geometric mean values calculated using mean values of fresh and aged test media per treatment group
RSD: relative standard deviation per treatment group n: number of analysed samples
n.a.: not applicable - Details on test conditions:
- Water Temperature:
The temperature was measured daily in an Erlenmeyer flask filled with water and incubated under the same conditions as the test flasks.
Water temperature was: 22-23°C
pH-Values:
The pH was measured in all test item concentrations and the control at the start and the end of the test.
pH was: 8.1 to 8.2 at test start and 8.0 to 10.0 at test end (see Table 10)
The pH in the control increased by more than 1.5 units in the control medium. However, this does not invalidate the tests since the validity criteria were met.
Light Regime:
Continuous illumination
Light Intensity:
The light intensity was measured once during the test at 6 positions distributed over the experimental area at the surface of the test media.
Mean light intensity: 5367 lux (range: 4900 to 5850 lux) - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- >= 197 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other: 179 -216 mg/L
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 312 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC20
- Effect conc.:
- >= 79.5 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other: 68 - 92.7 mg/L
- Duration:
- 72 h
- Dose descriptor:
- EC20
- Effect conc.:
- > 312 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 263 - >312
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- >= 49.5 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other: 39.9 - 61.1 mg/L
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- >= 161 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 125 - 207 mg/L
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 21.1 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 21.1 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- >= 92.4 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- >= 92.4 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The influence of SAT 150073, [2-[(2-methyl-1-oxoallyl)oxy]ethyl] hydrogen succinate on the growth of the freshwater green algae Pseudokirchneriella subcapitata was assessed in a static dose-response test. The 72-hour EyC50 was calculated to be 197 mg test item/L (geometric mean measured) and the 72-hour ErC50 value was assessed to be >312 mg test item/L (geometric mean measured). The 72-hour NOEyC was determined to be 21.1 mg test item/L (geometric mean measured) and the associated 72-hour LOEyC was 92.4 mg test item/L (geometric mean measured). The 72-hour NOErC was determined to be 21.1 mg test item/L (geometric mean measured) and the associated 72-hour LOErC was 92.4 mg test item/L (geometric mean measured).
- Executive summary:
The influence of SAT 150073, [2-[(2-methyl-1-oxoallyl)oxy]ethyl] hydrogen succinate on the growth of the freshwater green algae Pseudokirchneriella subcapitata was assessed in a static dose-response test. The 72-hour EyC50 was calculated to be 197 mg test item/L (geometric mean measured) and the 72-hour ErC50 value was assessed to be >312 mg test item/L (geometric mean measured). The 72-hour NOEyC was determined to be 21.1 mg test item/L (geometric mean measured) and the associated 72-hour LOEyC was 92.4 mg test item/L (geometric mean measured). The 72-hour NOErC was determined to be 21.1 mg test item/L (geometric mean measured) and the associated 72-hour LOErC was 92.4 mg test item/L (geometric mean measured).
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