kresoxim-methyl (ISO);methyl (E)-2-methoxyimino-[2-(o-tolyloxymethyl)phenyl]acetate

Toxicity to soil macroorganisms except arthropods

Administrative data

Endpoint:

toxicity to soil macroorganisms except arthropods: short-term

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1992

Report date:

1992

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 207 (Earthworm, Acute Toxicity Tests)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

methyl (2E)-2-methoxyimino-2-[2-[(2-methylphenoxy)methyl]phenyl]acetate

EC Number:

604-351-6

Cas Number:

143390-89-0

Molecular formula:

C18 H19 N O4

IUPAC Name:

methyl (2E)-2-methoxyimino-2-[2-[(2-methylphenoxy)methyl]phenyl]acetate

Details on test material:

- Name of test material (as cited in study report): Reg. No. 242 009
- Analytical purity: 93.7% active ingredient
- Batch No.: N 36

Test substrate

Vehicle:

no

Details on preparation and application of test substrate:

- Method of mixing into soil: Appropriate amounts of Reg. No. 242 009 were ground finely with 10 g of quartz sand and mixed thoroughly into moist artificial soil

Test organisms

Test organisms (species):

other: Eisenia foetida andrei

Animal group:

annelids

Details on test organisms:

TEST ORGANISM
- Common name: earthworm
- Source: Own culture, originally from " C.A.M. von Gestel, RIVM, NL-3720 Bilthoven, Netherlands "
- Age at test initiation: Adults with clitellum, less than 1 year old
- Weight at test initiation: >= 250 mg

ACCLIMATION
- Acclimation conditions: Animal were kept in boxes and fed with horse manure

Study design

Study type:

laboratory study

Substrate type:

artificial soil

Limit test:

no

Total exposure duration:

14 d

Test conditions

pH:

6.4

Moisture:

37% of the dry weight

Details on test conditions:

TEST SYSTEM
- Test container (material, size): glass jars, 1L
- Amount of soil or substrate: 750 g wet weight
- No. of organisms per container: 10
- No. of replicates per treatment group: 4
- No. of replicates per control: 4

SOURCE AND PROPERTIES OF SUBSTRATE
- Composition: 10% sphagnum peat, 20% Kaolin (kaolinite content > 30%), 1% CaCO3, 69% quartz sand (particle size > 80% 0.063 – 0.2 mm)

EFFECT PARAMETERS MEASURED: mortality after 7 and 14 days; weight after 0 and 14 days

TEST CONCENTRATIONS
- Range finding study: yes
- Test concentrations: control, 1, 10, 100, 500, 1000 mg/kg
- Results used to determine the conditions for the definitive study: no mortality in all treatment groups and no significant differences in weights compared to the control

Nominal and measured concentrations:

Nominal test concentrations: control, 93.7, 234.25, 468.5, 702.75, 937.0 mg/kg

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

14 d

Dose descriptor:

LC0

Effect conc.:

937 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mortality

Effect concentrations 2

Duration:

14 d

Dose descriptor:

LC50

Effect conc.:

> 937 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mortality

Effect concentrations 3

Duration:

14 d

Dose descriptor:

NOEC

Effect conc.:

937 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

other: biomass

Details on results:

The test substance caused neither mortality up to the highest concentration nor an effect on worm biomass.