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EC number: 274-492-2 | CAS number: 70236-62-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- Composite samples of each test concentration were drawn by mixing identical volumes of the test solutions taken from the approximate centre of the test vessels. They were taken immediately before exposure and after 48 hours exposure and kept at - 18 °C to - 22 °C until analysis.
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- - Stain: Stauss 1820
- Source: CIBA-GEYGY Ltd., testing facility
- Feeding: none during the test
- Breeding: Cultures of daphnia are maintained in glass vessels containing approx. 2.5 L of reconstituted water at 20 ± 1 °C (water is renewed partially thrice weekly). At each renewal the daphnia are fed with a suspension of green algae (Scenedesmus subspicatus) supplemented by a suspension of TETRAMIN-extract in such quantities that the food is consumed after 24h.
- Pretratment: 24 hours before the begin of the exposure reproductive daphnia are separated from the young by sieving all individuals through a 800 µm sieve. This operation is repeated immediately before the exposure and the young (0-24h of age) are retained for the test. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 240 mg CaCO3/l
- Test temperature:
- 20 ± 1 °C
- pH:
- 7.9 - 8.1
- Dissolved oxygen:
- 102 - 97 %
- Nominal and measured concentrations:
- Nominal concentrations: 4.4, 9.6, 21, 46, and 100 mg test substance/l
- Details on test conditions:
- TEST SYSTEM
- Test vessel: beakers covered with watch glasses; 100 ml solution per beaker
- Aeration: none
- Lighting: fluorescent light, 16 hours daily approx. 1500 lux
- Number of daphnia: 20 per concentration and control
- Replicates: 4 replicates of 5 daphnia each
- blank: water
TEST MEDIUM / WATER PARAMETERS
Reconstituted water containing:
- 65 mg NaHCO3
- 294 mg CaCl2 dihydrate
- 123 mg MgSO4 eptahydrate
- 6 mg KCl
in 1000 ml bidistilled water - Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 75 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95 % CL
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 18 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95 % CL
- Details on results:
- other values:
EC0 (48h) = NOEC= 4,4 mg/L
EC100 (48h) = 46 mg/L
The measured test substance concentrations were within the range 100 ± 20 % of the nominal concentrations, therefore the results were calculated based on nominal concentrations. - Results with reference substance (positive control):
- Immobilization in blank: 5 %
- Validity criteria fulfilled:
- yes
- Conclusions:
- EC50 (48h) = 18 mg/l (nominal)
- Executive summary:
The determination on ecotoxicity of the substance was determined in an Acute toxicity for Daphnia, test according to the guideline 92/69/EEC C.2. The test has been performed in compliance with the Procedures and Principles of Good Laboratory Practice. The test substance was tested on Daphnia magna Strauss 1820 for 48 hours.
20 daphnids (0-24 hours old) were exposed to nominal concentrations of 4.4, 9.6, 21, 46 and 100 mg test substance.
Results are:
EC50 (48 h) = 18 mg/l (nominal)
EC50 (24 h) calculated = 75 mg/l (nominal)
EC0 (48h) = NOEC = 4,4 mg/l (nominal)
EC100 (48h) = 46 mg/l (nominal)
Reference
Conc. Nominal mg/l |
Number of daphnia immobilized after 24h exposure | Number of daphnia immobilized after 48h exposure | ||||||
vessel 1 | vessel 2 | vessel 3 | vessel 4 | vessel 1 | vessel 2 | vessel 3 | vessel 4 | |
Blank | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
4,4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
9,6 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 0 |
21 | 1 | 2 | 0 | 0 | 3 | 2 | 2 | 3 |
46 | 2 | 0 | 1 | 2 | 5 | 5 | 5 | 5 |
100 | 4 | 2 | 3 | 4 | 5 | 5 | 5 | 5 |
Description of key information
EC50 (48 h) = 18 mg/l (nominal)
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 18 mg/L
Additional information
The short-term toxicity to invertebrates of the substance was determined in a short-term toxicity for Daphnia test, according to the guideline 92/69/EEC C.2. The test has been performed in compliance with the Procedures and Principles of Good Laboratory Practice. The test substance was tested on Daphnia Magna Strauss 1820 for 48 hours. 20 daphnids (0-24 hours old) were exposed to nominal concentrations of 4.4, 9.6, 21, 46 and 100 mg test substance. After 48b hours, no deaths occurred at the concentration of 4.4 mg/l. The EC50 at 48 hours has been staed to be 18 mg/l. The measured test substance concentrations were within the range 100 ± 20 % of the nominal concentrations, therefore the results were indicated based on nominal concentrations.
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