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Diss Factsheets
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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Adsorption / desorption
Administrative data
- Endpoint:
- adsorption / desorption: screening
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Recently conducted GLP compliant study using the most recent test methods
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 009
- Report date:
- 2009
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Type of method:
- HPLC estimation method
- Media:
- soil
Test material
- Details on test material:
- - Name of test material (as cited in study report): JKY-214
- Physical state: white solid
- Analytical purity: No information
- Lot/batch No.: Y002E
- Expiration date of the lot/batch: No information
- Stability under test conditions: No information. Assumed stable due to no data to the contrary
- Storage condition of test material: room temperature in the dark
- Other:
Constituent 1
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
Not Applicable - Radiolabelling:
- no
Study design
HPLC method
- Details on study design: HPLC method:
- EQUIPMENT
- Apparatus:
- Type: Agilent Technologies 1100, incorporating autosampler and workstation
- Type, material and dimension of analytical (guard) column: Zorbax SB CN 5pm (250 x 4.6 mm id)
- Column temperature: 40°C
- Detection system: UV
- Detector wavelength: dead time and reference standards: 210 nm; sample: 260 nm
MOBILE PHASES
- Type: methanol: water (55:45 v/v), adjusted with 0.1M hydrochloric acid
- Experiments with additives carried out on separate columns: no
- pH: 5.1
- Solutes for dissolving test and reference substances:
Test material (0.0201 g) was diluted to 100 ml with tetrahydrofuran
Solutions of reference standards were prepared in methanol.
DETERMINATION OF DEAD TIME
The dead time was determined by measuring the retention time of formamide (purity 99.94%, 622 mg/l solution in methanol).
REFERENCE SUBSTANCES
Standard Purity <%)* Concentration (mg/l)
Acetanilide 99+ 106
Phenol 99+ 106
Atrazine 97.2 102
Isoproturon 99.8 105
Triad i me nol 98.4 100
Linuron 99.7 100
Endosulfan-diol 99.8 115
Fenthion 97.8 103
a-Endosuifan 99.6 109
Phenanthrene 97.0 101
Diclofop-methyl 99.5 101
DDT 98.0 115
DETERMINATION OF RETENTION TIMES
- Quantity of test substance introduced in the column: 5 microlitres
- Quantity of reference substances: 5 microlitres
- Intervals of calibration: minutes between 2 and 35 minutes
REPETITIONS
- Number of determinations: 2 injections for sample and standards
EVALUATION
- Calculation of capacity factors k': k = tr-to/ to
where k = capacity factor, tr = retention time (mins) and to = dead time (mins)
- Calculation of retention times: recorded in mins and retention time of standards plotted to form calibration curve
- Determination of the log Koc value: log Koc = log k - A/ B
where Koc is adsorption coefficient, k = capacity factor, A = intercept of calibration curve and B = slope of calibration curve
Results and discussion
Adsorption coefficientopen allclose all
- Type:
- Koc
- Value:
- 5.35
- Type:
- log Koc
- Value:
- 218 000
Results: HPLC method
- Details on results (HPLC method):
- - Retention times of reference substances used for calibration:
The retention times of formamide and the retention times, capacity factors (k) and logio Koc values for the reference standards are shown in the two following tables:
Dead Time Retention 1 fime (mins) Mean Retention Time (mins)
Injection 1 Injection 2
Formamide 2.825 2.824 2.825
Standard Retention Time (mins) Mean Retention Time (mins) Capacity Factor (k) Logio k Log™ Koc
Injection 1 Injection 2
Acetanilide 3.724 3.723 3.724 0.318 -0.497 1.25
Phenol 3.566 3.566 3.566 0.263 -0.581 1.32
Atrazine 5.096 5.096 5.096 0.804 -9.46 x10~2 1.81
Isoproturon 5.314 5.316 5.315 0.882 -5.47 x10~2 1.86
Triadimenol 6.680 6.679 6.680 1.37 0.135 2.40
Linuron 7.052 7.046 7.049 1.50 0.175 2.59
Endosulfan-dio! 8.099 8.096 8.098 1.87 0.271 3.02
Fenthion 10.150 10.149 10.150 2.59 0.414 3.31
a-Endosulfan 13.841 13.811 13.826 3.90 0.591 4.09
Phenanthrene 11.728 11.716 11.722 3.15 0.498 4.09
Diclofop-methyl 15.209 15.195 15.202 4.38 0.642 4.20
DDT 28.782 28.810 28.796 9.20 0.964 5.63
- Details of fitted regression line (log k' vs. log Koc):
Log10Koc Log10k
1.250 -0.497
1.320 -0.581
1.810 -9.463E-Q2
1.860 -5.466E-02
2.400 0;135
2.590 0,175
3.020 0.271
3.310 0.414
4.090 0.591
4.090 0.498
4.200 0.642
5.630 0.864
A = >0.773
B = 0.330
r = 0.967
- Graph of regression line attached
- Average retention data for test substance:
Any other information on results incl. tables
The retention times of formamide and the retention times, capacity factors (k) and log10Kocvalues for the reference standards are shown in the two following tables:
Formamide retention time
|
Reference standards retention times
|
Adsorption coefficient of sample
The retention times, capacity factors and log10Kocvalues determined for the sample are shown in the following table:
|
Mean log10Koc: 5.34
Adsorption coefficient: 2.18 x 105
The method guideline states that the measurement of adsorption coefficient should be carried out on substances in their ionised and unionised forms. Testing was carried out at pH 5.1, with the test material in its non-ionised form and also attempted at a pH >9.24 in its ionised form. Analysis at the higher pH demonstrated drifting retention times indicative of damage caused to the stationary phase of the required column. Thus, only analysis performed in the non-ionised form was possible.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The adsorption coefficient (Koc) of the test material, in its non-ionised form, has been determined to be 21800, log10Koc5.34.
- Executive summary:
The soil adsorption coefficient has been assessed using the HPLC method according to EU method C19 in compliance with GLP. The test system was calibrated using formamide and the adsorption coefficient determined by reference to 12 reference reagents of known adsorption coefficient. Measurements for the test material were recorded in duplicate and the adsorption coefficient calculated by reference to the calibration curve established from the reference solutions. The adsorption coefficient was determined for the substance in its non-ionised form at pH 5.1. The test was attempted at higher pH, >9.41 to obtain the result for the ionised form, but the raised pH was considered to damage the stationary phase of the required column. The adsorption coefficient was determined to be 5.34 at pH 5.1.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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