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Toxicity to aquatic algae and cyanobacteria

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Description of key information

No adverse effects were seen at the lmit of solubility

Key value for chemical safety assessment

Additional information

The toxicity of Disperse Blue CVG to the unicellular freshwater green alga Pseudokirchneriella subcapitata was determined according to the principles of OECD 201, Commission Regulation (EC) No. 761/2009,C.3 and EPA Guideline 712-C-006: OCSPP 850.4500, 2012. The aim of the study was to assess the effects on biomass integrals, growth rate and yield over a period of 96 hours.

The study was conducted under static conditions with an initial cell density of 10010 cells/mL. The saturated solution and further four dilution levels were tested in a geometrical series with a dilution factor of 2:6.25 - 12.5 - 25.0 - 50.0-100 % of the saturated solution, corresponding to the nominal loading rates 0.313 - 0.625 - 1.25 - 2.50 - 5.00 mg/L. Four replicates were tested for each test item loading rate and eight replicates for the control. The environmental conditionswere within the acceptable limits.

No recovery phase was performed, because no loading level was inhibited by more than 50%.

 

The highest loading rate (saturated solution) of the test item Disperse Blue CVG and the control was analytically verified via HPLC-UV at the start of the exposure. The recovery rate of the stock solution at the start were below the limit of quantification.

All effect values were based on the nominal loading rates of the test item Disperse Blue CVG. The results are summarized in the following table:

 

NOEL, LOEL, EL-Values and 95 % Confidence Intervals of Disperse Blue CVG

    based on nominal test item loading rates

 

 

Inhibition of Biomass Integrals

Nominal test item loading rates [mg/L]

0 - 72 hours

0 - 96 hours

NOEL

5.00

5.00

LOEL

> 5.00

> 5.00

EbL10

> 5.00

> 5.00

EbL20

> 5.00

> 5.00

EbL50

> 5.00

> 5.00

 

Inhibition of Growth Rate

Nominaltest item loading rates [mg/L]

NOEL

5.00

5.00

LOEL

> 5.00

> 5.00

ErL10

> 5.00

> 5.00

ErL20

> 5.00

> 5.00

ErL50

> 5.00

> 5.00

 

Inhibition of Yield

Nominal test item loading rates [mg/L]

NOEL

5.00

5.00

LOEL

> 5.00

> 5.00

EyL10

> 5.00

> 5.00

EyL20

> 5.00

> 5.00

EyL50

> 5.00

> 5.00