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EC number: 812-038-2 | CAS number: 1192651-80-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2008
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- publication
- Title:
- The ecotoxicity of ionic liquids and traditional organic solvents on microalga Selenastrum capricornutum
- Author:
- Cho, CW, Jeon, YC, Pham, TPT, Vijayaraghavan, K, Yun, YS
- Year:
- 2 008
- Bibliographic source:
- Ecotoxicology and Environmental Safety. volume 71, pp 166–171
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- not specified
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 850.5400 (Algal Toxicity, Tiers I and II) (January 2012)
- Deviations:
- not specified
- GLP compliance:
- no
Test material
- Reference substance name:
- Tetrabutylammonium bromide
- EC Number:
- 216-699-2
- EC Name:
- Tetrabutylammonium bromide
- Cas Number:
- 1643-19-2
- Molecular formula:
- C16H36N.Br
- IUPAC Name:
- N,N,N-tributylbutan-1-aminium bromide
- Test material form:
- not specified
- Details on test material:
- - Name of test material (as cited in study report): Tetrabutylammonium bromide
- Molecular formula: (CH3CH2CH2CH2)4N(Br)
- Molecular weight: 322.37
- Analytical purity: >99.5%
- Lot/batch No.: Not reported
- Expiration date of the lot/batch: Not reported
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- The test substance was completely miscible with water, forming a homogeneous phase.
Test organisms
- Test organisms (species):
- Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- The green microalgae used as the model algal strain in this study, Selenastrum capricornutum ATCC-22662, were obtained from the National
Institute Environmental Research, Korea. The algae stock was cultivated in 250 mL Erlenmeyer flasks, containing 200 mL sterilized nitrate-enriched
BBM medium prepared in triple distilled water, to avoid nitrogen limitation during the high-density culture.
The culture flask was agitated on a shaker at 170 rpm, and bubbled with air (1 vvm), without sparger. Light was continuously supplied, with an
average of 30±5 µEm-2 s-1, using warm-white fluorescent tubes (Korea General Electric Co., Korea) located on top of the shaker. All the flasks
were maintained in the shaker incubator at 25±5 °C for 7 days.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
Test conditions
- Hardness:
- Not reported
- Test temperature:
- 25 °C
- pH:
- Not reported
- Dissolved oxygen:
- Not reported
- Nominal and measured concentrations:
- Nominal concentrations between 0.1M and 1.26 µM were tested to obtain complete concentration response curves.
- Details on test conditions:
- Experiments were performed in 250 mL Erlenmeyer flasks, containing 55 mL of sterilized culture medium, inoculated with 5 mL samples of 7-day cultured algae. Solutions of ionic liquids and organic solvents were subsequently added to the test flasks. The ionic liquids and organic solvents tested were completely miscible with water, forming a homogeneous phase. Concentrations of these toxicants were adjusted in the range between 0.1M and 1.26 µM to obtain complete concentration response curves. The flasks then were placed on a shaker incubator at 170 rpm and 25°C, with 24 h light supplied via warm-white fluorescent tubes, with an average illumination of 30±5 µEm-2 s-1. At each determined exposure date, the optical density of the algal biomass was estimated at 438nm using a spectrophotometer (UV mini-1240, Shimadzu, Kyoto, Japan). The dry cell weight, corresponding to the optical density, was determined from the linear relationship; dry cell weight (g/l) = 0.1329x optical density. All experiments were performed in duplicate, but the control experiments were conducted in triplicate.
- Reference substance (positive control):
- no
Results and discussion
Effect concentrations
- Duration:
- 96 h
- Dose descriptor:
- EC50
- Effect conc.:
- 722 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: Reported as Log10 EC50(µM): 3.35 +/- 0.25
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The nominal 96h-EC50 value of Tetrabutylammonium bromide to Pseudokirchneriella subcapitata was 722 mg/L.
- Executive summary:
The toxicity of Tetrabutylammonium bromide to Pseudokirchneriella subcapitata was determined in accordance with OECD 201 and EPA OPPTS 850.4500 (Tier I and II) guidelines (non-GLP).
Nominal concentrations were not confirmed by analytical measurements. However, since the substance is expected to be stable in an algae study, it can be assumed that the exposure was constant.
The nominal 96h-EC50 value of Tetrabutylammonium bromide was 722 mg/L.
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