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Environmental fate & pathways

Biodegradation in water: screening tests

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Reference
Endpoint:
biodegradation in water: screening tests
Type of information:
experimental study
Adequacy of study:
key study
Study period:
16 October to 12 November 1992
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
EU Method C.9 (Biodegradation: Zahn-Wellens Test)
GLP compliance:
yes
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge (adaptation not specified)
Details on inoculum:
A sample of activated sludge was taken from an oxidation ditch situated on the premises of TNO, Delft, The Netherlands. The oxidation ditch is used to treat domestic sewage.
The original sludge (containing 3.9 g of solid substance) was allowed to settle for 5 minutes and 220 ml of the supernatant was used to inoculate 18 litre of BOD dilution water. The inoculated dilution water was aerated vigorously before use.
Duration of test (contact time):
28 d
Key result
Parameter:
% degradation (O2 consumption)
Value:
6
Sampling time:
28 d
Validity criteria fulfilled:
yes
Interpretation of results:
under test conditions no biodegradation observed
Conclusions:
Under the conditions of the test the substance is not readily biodegradable.
Executive summary:

The ready biodegradation of the substance was assessed according to the EC C8 and C9 test method in accordance with GLP. The test has been conducted using activated sludge from a domestic system as inoculum. The substance is highly water soluble and stable in water. Under the condictions of the test, 6% degradation has occured and the substance is considered to be not readily biodegradable.

Description of key information

The ready biodegradation of the substance was assessed according to the EC C8 and C9 test method in accordance with GLP.  The test has been conducted using activated sludge from a domestic system as inoculum.  The substance is highly water soluble and stable in water.  Under the condictions of the test, 6% degradation has occured and the substance is considered to be not readily biodegradable.

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed

Additional information

The ready biodegradation of the substance was assessed according to the EC C8 and C9 test method in accordance with GLP. The test has been conducted using activated sludge from a domestic system as inoculum. The substance is highly water soluble and stable in water. Under the condictions of the test, 6% degradation has occured and the substance is considered to be not readily biodegradable.

The biodegradability of sodium p-styrenesulfonate is 38%, >95% and 31% calculated based on BOD with OECD Guidelines for the Testing of Chemicals 302C Inherent Biodegradability Modified MITI TEST (II)(1981). Sodium p-styrenesulfonate is inherently biodegradable under this experimental conditions.