α-hydroxy-β,β-dimethyl-γ-butyrolactone

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: The test was carried out according to German national standards, however no information was given on the purity of the test substance.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: German standard DIN 38412 L9

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

α-hydroxy-β,β-dimethyl-γ-butyrolactone

EC Number:

209-963-3

EC Name:

α-hydroxy-β,β-dimethyl-γ-butyrolactone

Cas Number:

599-04-2

Molecular formula:

C6H10O3

IUPAC Name:

(3R)-dihydro-3-hydroxy-4,4-dimethyl-2(3H)-furanone

Details on test material:

- Name of test material (as cited in study report): D-Pantolactone
Purity: no data
Water solubility: 950 g/l

Sampling and analysis

Analytical monitoring:

no

Test solutions

Details on test solutions:

Each test concentration was prepared separately.

Test organisms

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

TEST ORGANISM
- Strain:SAG 86.81
- Source (laboratory, culture collection): Algae collection Goettingen, Germany

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Test conditions

Test temperature:

21.4 °C

pH:

7.75 - 7.99

Nominal and measured concentrations:

Nominal test concentrations: 0 (control), 25, 50, 100, 250, and 500 mg/l

Details on test conditions:

TEST SYSTEM
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): 4



TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to DIN 38412 L9



EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: fluormetrically

Reference substance (positive control):

yes

Remarks:

Potassium chromate p.a.

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

> 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Reported statistics and error estimates:

Statistics were performed according to Tallarida RJ and Jacob LS, The dose-response relation in pharmacology, 98 -103, Springer

Any other information on results incl. tables

ECx values (in mg/l) after 72 hours of exposure

ErC10  > 500 mg/l
ErC50  > 500 mg/l

EbC10  = 211.61 mg/l
EbC50  > 500 mg/l