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EC number: 224-748-4 | CAS number: 4474-24-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: screening test, other
- Remarks:
- inherentl biodegradation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- June 10, 1991
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
- Qualifier:
- according to guideline
- Guideline:
- other: Safepharm Standard Method Number FT/ETAD02/90
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)
- GLP compliance:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge: Severn Trent Plc sewage treatment plant at Belpe
- Pretreatment: the sample was maintained at 21 °C with continuous aeration and washed by settlement and resuspension in test medium prior to use.
- Concentration of sludge: equivalent to 1.0 g dry weight/l. - Duration of test (contact time):
- ca. 28 d
- Initial conc.:
- ca. 100 mg/L
- Based on:
- act. ingr.
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- TEST SOLUTION
2.5 g of test material was dispersed in deionised water and the volume adjusted to 1 liter to give a 2000 mg/l active ingredient stock dispersion. 100 ml of this was added to the vessel to give a test concentration of 100 mg a.i/l.
TEST CONDITIONS
- Vessel: 3 l glass beker
- Test temperature: 22 ± 1 °C
- pH: 7-8
- pH adjusted: yes, with NaOH of H2SO4 as necessary
- Continuous darkness: yes
- Other: losses by evaporation were made good by the addition of deionised water immediately prior to sampling.
SAMPLING
Aeration of the test vessels was stopped and the contents of the vessels allowed to Bettle for 20 minutes. Samples (appoximately 20 ml) were withdrawn from the supernatent and centrifuged at 3900 rpm for 15 minutes to remove any further suspended solids. Samples were analysed immediately. Samples were taken at 0 and 3 hours and an Days 7, 14, 21, 27 and 28 for dissolved organic carbon and spectrophotometric analysis. - Reference substance:
- diethylene glycol
- Parameter:
- % degradation (DOC removal)
- Value:
- > 4 - < 16
- Sampling time:
- 28 d
- Results with reference substance:
- Digol attained 100 % degradation within 14 days.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not inherently biodegradable
- Conclusions:
- Biodegradation based on DOC removal within 28 days is between 4 and 16 %
- Executive summary:
Methods:
The inherent biodegradability of test substance was determined in a 28 days Zahn-Wellens -test according to the OECD Guideline for Testing of Chemicals, No. 302B and according to Safepharm Standard Method Number FT/ETAD02/90.
Results:
Bioelimination of test substance measured as DOC removal after 28 days is between 4 and 16 %.
Reference
Percentage of biodegradation for Didigol and test item calculated from DOC levels:
Day | DOC value mg/l (mean of 3 Assays) | |
Test substance | Didigol | |
3 h | 4 | 2 |
7 | 14 | 98 |
14 | 14 | 100 |
21 | 16 | 100 |
27 | 11 | 99 |
28 | 4 | 99 |
Description of key information
Not readily biodegradable
Key value for chemical safety assessment
Additional information
The inherent biodegradability of test substance was determined in a 28 days Zahn-Wellens -test according to the OECD Guideline for Testing of Chemicals, No. 302B and according to Safepharm Standard Method Number FT/ETAD02/90.
The bioelimination of test substance measured as DOC removal after 28 days is between 4 and 16 %, so it is possible to conclude that the test substance is not Inherently biodegradable.
In a supporting study the substance has been tested to evaluate the BOD5/COD ratio. The test has been conducted in 1974 according to the German guideline DEV H4 and DEV H5.
Biochemical Oxygen Demand (BOD5) and Chemical Oxygen Demand (COD) are the most commonly used parameters for the characterization of the biodegradability capacity.
COD results are typically higher than BOD5 values, and their ratio vary depending on the characteristics of the substrate. This ratio has been commonly used as an indicator for biodegradation capacity, the "Biodegradability index" (B.I.).
As indicated in ECHA Guidance requirement R.7B, the BOD5 test is a traditional biodegradation test that is now replaced by the ready biodegradability tests. Therefore, this test should not be performed today for assessment of the ready biodegradability of substances.
Older test data may, however, be used when no other degradability data are available.
For the assessment it is only available a summary on BOD5 and COD.
COD was measured according to the German guideline DEV H4. The COD measured was 1180 mg/g O2.
BOD5 was measured according to the German guideline DEV h5. The BOD5 measured was 39 mg/g O2, at concentration of 100 mg/L.
As reported in the ECHA Guidance R7.b, a substance is considered as readily biodegradable if BOD5/COD > = 0.5. The ratio BOD5/COD for the test item is 0.03, therefore the substance is considered as not readily biodegradable
This result is in line with the one obtained in the key study above described.
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