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Diss Factsheets
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EC number: 200-270-1 | CAS number: 56-37-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms, other
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Justification for type of information:
- Data for the target chemical is summarized data from handbook or collection of data
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- other: as mentioned below
- Principles of method if other than guideline:
- WoE report is based on two toxicity study of microorganism for the test chemical :
1.Immobilization of Candida rugosa lipase on bentonite was observed which is modified with test chemical.
2.Toxicity to V. fischeri was measured as inhibition of bioluminescence using Microtoxs M500 Rapid Toxicity Testing System equipment and consumables.The assay was carried out in accordance with the 90% basic test for pure compounds protocol, as described in the Microtox user’s manual. - GLP compliance:
- not specified
- Analytical monitoring:
- yes
- Vehicle:
- yes
- Details on test solutions:
- 1.PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)- Chemical name of vehicle (organic solvent, emulsifier or dispersant):sodium bentonite-Modification of the bentonite clay was carried out via the exchange of the quaternary ammonium cations of the benzyltriethylammonium ion (BTEA+).
- Test organisms (species):
- other: 1) Candida sps. 2) Vibrio fisheri
- Details on inoculum:
- 1.Candida rugosa lipase (CRL)- Initial biomass concentration:≥700 units/mg solid
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 15 min
- Test temperature:
- 35-45 degrees C
- pH:
- 5.5-7.0
- Details on test conditions:
- 1) For the synthesis of the benzyltriethylammonium chloride (BTEAC)organoclay, two concentrations of the quaternary ammonium salt were used, 1.0 and 2.1 equivalents of the CEC of the Na-Bent. Sodium bentonite in water (2%, w/v) was mixed with a BTEAC solution until a clay-solution ratio of 1:100 g/mL was obtained.
- Dose descriptor:
- EC50
- Effect conc.:
- >= 117.8 - <= 511.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Remarks on result:
- other: Immobilization effects showed by modified clay of benzyltriethylammonium chloride using 1.0 and 2.1 times the cation exchange capacity (CEC) of the clay.
- Key result
- Duration:
- 15 min
- Dose descriptor:
- EC50
- Effect conc.:
- 284 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Remarks on result:
- other: Other details not known
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The test chemica is not likely to be toxic to microorganism atleast in the dose range of 100-405.0 mg/l
- Executive summary:
Data available for the functionally similar read across chemicals has been reviewed to determine the short term toxicity of microorganism of the test chemical .The studies are as mentioned below:
In the first study the organobentonites were prepared from sodium bentonite by interchange with test material, using 1.0 and 2.1 times the cation exchange capacity (CEC) of the clay. The enzymatic activities of the lipase immobilized in the two organobentonites were 511.5 and 177.8 U/g support, while the activity recoveries were 33.5% and 14.7%. C. rugosa lipase immobilized on BTEA-1.0-Bent was found to be the optimal system studied, and it showed the greatest activity recovery with respect to the hydrolysis of olive oil (33.5%). This result is due to the smaller charge compensated by the organic cations, allowing for the dispersion of the lipase on the support, preventing its aggregation and maintaining its activity. However, the CRL/BTEA-2.1-Bent system showed a higher thermal stability between the range of 35 and 45 ◦C and pH 6.5.
Above data is supported by the another data from journal. Toxicity to V. fischeri was measured as inhibition of bioluminescence using Microtoxs M500 Rapid Toxicity Testing System equipment and consumables.The assay was carried out in accordance with the 90% basic test for pure compounds protocol, as described in the Microtox user’s manual. The EC50 values of the tested chemical for V. fischeri range from 1.3 to 284 mg L1. The differences tested by ANOVA are highly significant (P o 0.001). In experiment the 50% effect concentrations (EC50) of the test chemical to Vibrio fischeri in a 15 min inhibition of bioluminescence test was observed to be 284 mg/l with 95% confidence limit (241–336).
Hence,based on the data available for the functionally similar read across, test chemical is not likely to be toxic atleast in the dose range of 511 mg/L.
Reference
Description of key information
The test chemica is not likely to be toxic to microorganism atleast in the dose range of 100-405.0 mg/l
Key value for chemical safety assessment
- EC50 for microorganisms:
- 284 mg/L
Additional information
Data available for the functionally similar read across chemicals has been reviewed to determine the short term toxicity of microorganism of the test chemical .The studies are as mentioned below:
In the first study the organobentonites were prepared from sodium bentonite by interchange with test material, using 1.0 and 2.1 times the cation exchange capacity (CEC) of the clay. The enzymatic activities of the lipase immobilized in the two organobentonites were 511.5 and 177.8 U/g support, while the activity recoveries were 33.5% and 14.7%. C. rugosa lipase immobilized on BTEA-1.0-Bent was found to be the optimal system studied, and it showed the greatest activity recovery with respect to the hydrolysis of olive oil (33.5%). This result is due to the smaller charge compensated by the organic cations, allowing for the dispersion of the lipase on the support, preventing its aggregation and maintaining its activity. However, the CRL/BTEA-2.1-Bent system showed a higher thermal stability between the range of 35 and 45 ◦C and pH 6.5.
Above data is supported by another data from journal. Toxicity to V. fischeri was measured as inhibition of bioluminescence using Microtoxs M500 Rapid Toxicity Testing System equipment and consumables. The assay was carried out in accordance with the 90% basic test for pure compounds protocol, as described in the Microtox user’s manual. The EC50 values of the tested chemical for V. fischeri range from 1.3 to 284 mg L1. The differences tested by ANOVA are highly significant (P o 0.001). In experiment the 50% effect concentrations (EC50) of the test chemical to Vibrio fischeri in a 15 min inhibition of bioluminescence test was observed to be 284 mg/l with 95% confidence limit (241–336).
Hence, based on the data available for the functionally similar read across, test chemical is not likely to be toxic at least in the dose range of 511 mg/L.
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