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EC number: 226-024-3 | CAS number: 5223-06-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP - guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 005
- Report date:
- 2005
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 5-ethylpyridine-2-ethanol
- EC Number:
- 226-024-3
- EC Name:
- 5-ethylpyridine-2-ethanol
- Cas Number:
- 5223-06-3
- Molecular formula:
- C9H13NO
- IUPAC Name:
- 2-(5-ethylpyridin-2-yl)ethan-1-ol
- Test material form:
- other: viscous liquid
- Details on test material:
- - Name of test material (as cited in study report) : 5-Ethyl-2-(2-hydroxyethyl)-pyridin
- Physical state : clear , viscous liquid
- Analytical purity : 96,5%
- Lot/batch No. : 07040163/102
- Expiration date of the lot/batch : 2005-12-15
- Storage condition of test material : room temperature , closed original container , protected from light
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- sewage, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure) : Effluent of the treatment plant "Gemeinschaftsklärwerk Bitterfeld-Wolfen" , receiving predominantly domestic sewage
- Pretreatment : A fresh sample was allowed to settle for 1 hour and the decanted liquid was used for the test in a concentration of 0,6 ml/l . - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 2.5 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- other: oxygen evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium : The test item was dispersed in mineral medium in a concentration of 2,5 mg/l , the inoculum was added and the mixture was filled into the BOD bottles , so that the bottles were completely filled .
- Test temperature : 20,0°C
- pH adjusted : no
- Continuous darkness : yes
TEST SYSTEM
- Culturing apparatus : BOD bottles , stoppered
- Number of culture flasks/concentration : 2
- Measuring equipment : dissolved oxygen was analysed by the electrode method
SAMPLING
- Sampling frequency : after 0, 2, 5, 9, 13, 16, 19, 23, 26 and 28 days
CONTROL AND BLANK SYSTEM
- Inoculum blank : yes
- Abiotic sterile control : -
- Toxicity control : yes
Reference substance
- Reference substance:
- benzoic acid, sodium salt
- Remarks:
- (3,3 mg/l)
Results and discussion
% Degradation
- Parameter:
- other: oxygen evolution
- Value:
- > 0 - <= 9
- Sampling time:
- 28 d
- Details on results:
- For the test item , no marked reduction of the oxygen concentration was observed . Throughout the experiment small values of oxygen uptake were measured . As the results range from 0 to 9 % and do not exceed 9 % at any time they can not be interpreted as a degradation of the test item .
BOD5 / COD results
- Results with reference substance:
- On day 2 the reference substance reached the pass level for ready biodegradability (60%) . The result is the criteria for normal testing .
Any other information on results incl. tables
Results of dissolved oxygen analysis
Day | Concentration of dissolved oxygen (mgO2/l) | |||
Inoculum control | Procedure control | Test item | Toxicity control | |
0 | 9,0 | 9,0 | 9,0 | 9,0 |
9,0 | 9,0 | 9,0 | 9,0 | |
2 | 8,8 | 5,3 | 8,7 | 5,4 |
8,7 | 5,1 | 8,6 | 5,2 | |
5 | 8,5 | 4,3 | 8,4 | 4,2 |
8,6 | 4,2 | 8,5 | 4,1 | |
9 | 8,6 | 4,0 | 8,3 | 3,7 |
8,5 | 4,0 | 8,5 | 3,7 | |
13 | 8,4 | 3,9 | 8,1 | 3,4 |
8,4 | 3,9 | 8,2 | 3,5 | |
16 | 7,8 | - | 8,0 | - |
7,8 | - | 7,8 | - | |
19 | 7,5 | - | 7,6 | - |
7,5 | - | 7,5 | - | |
23 | 7,6 | - | 7,1 | - |
7,6 | - | 7,0 | - | |
26 | 7,4 | - | 7,3 | - |
7,5 | - | 7,2 | - | |
28 | 7,7 | - | 7,3 | - |
7,4 | - | 7,6 | - |
Calculated oxygen depletion
Day | Oxygen depletion (mgO2/l) | |||||
Procedure control | Test item | Toxicity control | ||||
Bottle 1 and 2 | Mean | Bottle 1 and 2 | Mean | Bottle 1 and 2 | Mean | |
2 | 3,45 | 3,55 | 0,05 | 0,10 | 3,35 | 3,45 |
3,65 | 0,15 | 3,55 | ||||
5 | 4,25 | 4,30 | 0,15 | 0,10 | 4,35 | 4,40 |
4,35 | 0,05 | 4,45 | ||||
9 | 4,55 | 4,55 | 0,25 | 0,15 | 4,85 | 4,85 |
4,55 | 0,05 | 4,85 | ||||
13 | 4,50 | 4,50 | 0,30 | 0,25 | 5,00 | 4,95 |
4,50 | 0,20 | 4,90 | ||||
16 | - | - | -0,20 | -0,10 | - | - |
- | 0 | - | ||||
19 | - | - | -0,10 | -0,05 | - | - |
- | 0 | - | ||||
23 | - | - | 0,50 | 0,55 | - | - |
- | 0,60 | - | ||||
26 | - | - | 0,15 | 0,20 | - | - |
- | 0,25 | - | ||||
28 | - | - | 0,25 | 0,10 | - | - |
- | -0,05 | - |
Degradation of the test item and reference substance
Day | Degradation (%) | |
Procedure control | Test item | |
2 | 64 | 2 |
5 | 78 | 2 |
9 | 83 | 3 |
13 | 82 | 4 |
16 | - | 0 |
19 | - | 0 |
23 | - | 9 |
26 | - | 3 |
28 | - | 2 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- The percentage degradation of the reference compound has reached the pass level of 60% on day 2; The residual concentration of oxygen did not fall below 0.5 mg/l
- Interpretation of results:
- other: not readiliy biodegradable
- Conclusions:
- The test substance was tested for the ready biological degradability in Closed Bottle Test. The test was performed according to EEC Directive 92/69/EEC, Annex V, Determination of the Ready Biodegradability, C.4E Closed Bottle Test without deviations and therefore considered to be of the highest quality (reliability Klimisch 1). The validity criteria of the test system are fulfilled. In the present study no marked oxygen uptake by the test item was measured . The pass level for ready biodegradation (60% degradation) was not reached . For this reason in the test for ready biodegradability the test item seems to be not ready biodegradable .
- Executive summary:
The biodegradability of the substance 5-Ethyl-2-(2-hydroxyethyl)-pyridin was investigated according to EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test). The study was conducted in compliance with the Principles of Good Laboratory Practice (GLP). Mineral medium containing a known concentration of the test item was inoculated with micro organisms from the effluent of a treatment plant (predominantly domestic sewage) and incubated in the dark over a 28 day period. Degradation was followed by oxygen evolution twice a week. The amount of oxygen taken up by the microbial population during biodegradation of the test item is expressed as a percentage of the theoretical oxygen demand of the test item. For the test item, no marked reduction of the O2 concentration was observed. Throughout the experiment small values of O2 uptake were measured. As the results range from 0 to 9 % and do not exceed 9 % at any time they can not be interpreted as a degradation of the test item.. The result of a toxicity control showed that the concentration of test item tested had no inhibitory effect to the inoculum. The pass level for ready biodegradation (60 % degradation) was not reached. Thus in the test for ready biodegradability the test item seems to be not readily biodegradable.
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