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EC number: 217-288-0 | CAS number: 1800-91-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From August 2017 to January 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- - Appearance: Clear colourless liquid
- Test item storage: At room temperature protected from light
- Purity/Composition correction factor: No correction factor required - Analytical monitoring:
- yes
- Details on sampling:
- Samples were taken from test group of fresh media at 0 and 72 hours, and of old media at 24 and 96 hours.
- Vehicle:
- no
- Details on test solutions:
- Test item was added directly into test water to prepare the test solution. The weight of test item was calculated according to the volume of bottles; 1.13231-1.15963 g the test item was weighted and moved into a glass bottle (with a stopper) respectively. Test water of 11.3
-11.6 L was filled into the bottles respectively, and then be magnetic stirred (350 - 420 rpm) for about 24 hours to get the test solution. Following the stirring period, the test solution was settled for about 24 hours, and then the upper 1/3 solution was taken to use for test by siphon device. During this process, the closed system was used to prevent the volatilization of the test item. - Test organisms (species):
- other: Gobiocypris rarus
- Details on test organisms:
- TEST ORGANISM
- Common name: Gobiocypris rarus
- Source: in-house culture
- Length at study initiation: 2.3 to 2.9 cm
- Weight at study initiation: 0.2346 - 0.3012
ACCLIMATION
- Acclimation period: more than 14 days:
- Temperature: 21-25°C (constant within a range of 2°C)
- pH: 6.0-8.5
- Dissolved oxygen concentration: > 80% of air saturation value (ASV)
- Photoperiod: light/dark = 12 hours/12 hours;
- Feeding: brine shrimp twice on workday and once on weekend which was discontinued approximately 24 hours prior to the start of the definitive test
- Health during acclimation (any mortality observed): There were no any sign of disease or mortalities maintained in-house since June 13, 2016 and the fish were held in water of the quality to be used in the test during the acclimation period and under the following conditions:
FEEDING DURING TEST : none - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Test temperature:
- 22.07 - 23.36°C.
- pH:
- 8.10 - 8.34
- Dissolved oxygen:
- 86.9-100.5% of the air saturation value (ASV)
- Nominal and measured concentrations:
- Nominal concentrations (limit test): 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Aeration: continuous over 24h
- Renewal rate of test solution: every 24h
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: <1.0 g fish /L water
TEST MEDIUM / WATER PARAMETERS :
Source/preparation of dilution water: The water used for the aquatic test was water treated with aquapro pure water system
OTHER TEST CONDITIONS
- Photoperiod: light/dark = 12 hours/12 hours
EFFECT PARAMETERS MEASURED : The fish in the test and control groups were observed daily. Toxic signs and mortality were recorded at 2, 4, 24, 48, 72 and 96 hours after exposure.
OTHER PARAMETERS MEASURED: Dissolved oxygen concentrations and pH-values of fresh and old media were measured daily during the 96-hour exposure period in both control and test solution. The environmental temperature was monitored by automatic temperature monitoring system and recorded at 1 hour interval.
LIMIT TEST CONCENTRATIONS
- Range finding study : not performed, only a limit test was performed
- Test concentrations: 100 mg/L (nominal)
- Results used to determine the conditions for the definitive study: yes - Reference substance (positive control):
- yes
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LL50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- NOELR
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 0.62 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 0.62 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- ANALYTICAL RESULTS:
For the test solutions of nominal concentrations of 100 mg/L, the mean determined concentration was 0.66 mg/L in the fresh solutions and 0._59 mg/L in the old solutions. The mean measured concentration was 0.62 mg/L.
TOXIC SIGNS and MORTALITY:
During the 96-hour exposure period, the fish showed no poisoning signs or deaths in control vessels and 100 mg/L vessels - Sublethal observations / clinical signs:
ANALYTICAL RESULTS:
For the test solutions of nominal concentrations of 100 mg/L, the mean determined concentration was 0.66 mg/L in the fresh solutions and 0.59 mg/L in the old solutions. The mean measured concentration was 0.62 mg/L. Results are shown in the table below
Nominal concentration
(mg/L)
Replicate
Measured Concentration of sample
(mg/L)
Mean in fresh media (mg/L)
Mean in old media (mg/L)
Overall conc. (mg/L)
2016-08-29
2016-09-01
0h-Fresh
24h-old
72h-Fresh
96h-old
Control
1
<LOD
0.100*
<LOD
<LOD
-
-
-
100
1
0.67
0.57
0.75
0.55
2
0.63
0.61
0.73
0.62
0.66
0.59
0.62
3
0.62
0.54
0.57
0.63
Mean
0.64
0.57
0.68
0.60
LOD :0.1 mg/L.
* the sample of control was suspected contaminated when pretreated because signal wasn't detected in the 0h sample.
TOXIC SIGNS and MORTALITY:
During the 96-hour exposure period, the fish showed no poisoning signs or deaths in control vessels and 100 mg/L vessels.
Acceptability of the Test
1.No mortality was observed in the control during the test.
2. The dissolved oxygen concentrations in test solutions were between 86.9%- 100.5% ASV,which was greater than 60.0%of the air saturation value, throughout the test.
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of the test, according to the lack of toxicological signs and death, the 96h-LC50 and the 96h-NOEC for Gobiocypris rarus exposed to the test item was determined to be > 0.62 mg/L and >= 0.62 mg/L based on measured concentrations of the limit test concentration (loading rate of 100 mg/L), respectively.
- Executive summary:
The acute toxicity of the test item to the freshwater fish Gobiocypris rarus was investigated in a GLP-compliant study performed in accordance with OECD Guidelines No. 203. Under the conditions of the test, according to the lack of toxicological signs and death, the 96h-LC50 and the 96h-NOEC for Gobiocypris rarus exposed to the test item was determined to be > 0.62 mg/L and = 0.62 mg/L based on measured concentrations of the limit test concentration (loading rate of 100 mg/L), respectively.
It could be concluded that no toxicity was observed at the limit of solubility of the test item in the test medium.
Reference
Description of key information
The acute toxicity of the test item to the freshwater fish Gobiocypris rarus was investigated in a GLP-compliant study performed in accordance with OECD Guidelines No. 203. Under the conditions of the test, according to the lack of toxicological signs and death, the 96h-LC50 and the 96h-NOEC for Gobiocypris rarus exposed to the test item was determined to be > 0.62 mg/L and = 0.62 mg/L based on measured concentrations of the limit test concentration (loading rate of 100 mg/L), respectively.
It could be concluded that no toxicity was observed at the limit of solubility of the test item in the test medium.
Key value for chemical safety assessment
Additional information
The toxicity of the test item to freshwater fish was investigated in one GLP-compliant study performed in accordance with standard methods, without deviations. The study is considered as reliable (Klimisch 1) and is selected as a key study for the endpoint.
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