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EC number: 406-200-8 | CAS number: 117827-06-2 COUPLER II; UC-136
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- from 1989, august 22nd to 1989, september 20th
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP study performed according to the Test Guidelines described in the EEC Directive 84/449/EEC.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 989
- Report date:
- 1989
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.5 (Degradation: Biochemical Oxygen Demand)
- GLP compliance:
- yes
Test material
- Reference substance name:
- 3',5'-dichloro-4'-ethyl-2'-hydroxypalmitanilide
- EC Number:
- 406-200-8
- EC Name:
- 3',5'-dichloro-4'-ethyl-2'-hydroxypalmitanilide
- Cas Number:
- 117827-06-2
- Molecular formula:
- C24H39Cl2NO2
- IUPAC Name:
- N-(3,5-dichloro-4-ethyl-2-hydroxyphenyl)hexadecanamide
- Details on test material:
- Identification UC—136
RCC NOTOX Substance 1763
Description White powder
Purity 98.5%
Instructions for test article storage : At room temperature in the dark in a tightly sealed container
Stability of test article : Stable for one year maximal under storage conditions
Safety precautions : Gloves, goggles and face mask will be sufficient to ensure personnel health and safety
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, adapted
- Details on inoculum:
- Inoculum
The source of test organisms was activated
sludge freshly obtained from a municipal
sewage treatment plant, Waterschap de Aa,
Schijndel, The Netherlands.
Treatment
The activated sludge was aerated for 4 hours.
It was then left to settle for about 1 hour.
The supernatant was decanted to provide a
sufficient large volume for a 1% inoculum for
each CO2 test flask.
Temperature
20 ± 2°C
Reason for selection
This test has been found suitable by the OECD
Expert Group Degradation! Accumulation for
determining the ready biodegradability of
organic chemicals under aerobic conditions.
It has been tested in the OECD Laboratory
Intercomparison Test Programme (1978—1980).
Because of the stringency of this test method
a result of less than 60% yield of CO2
(within 28 days) does not necessary mean that
the test substance is not biodegradable under
environmental conditions, but indicates that
more work will be necessary to establish
biodegradability. - Duration of test (contact time):
- 28 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- 10 mg/L
- Based on:
- test mat.
- Initial conc.:
- 20 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- Total carbon content
The theoretical carbon content was calculated from the bruto formula.
Pre—incubation test medium
30 ml of the inoculum was added to the test medium present in each of the 3 litre test
bottles. This mixture was aerated with CO2 free air for 24 hours.
Preparations
After the aeration period, three CO2 absorber bottles were filled with 80 ml 0.025 N
Ba(OH)2 and connected in series to the exit air line of each test bottle.
Test material was added to two bottles, nominal concentrations 10 and 20 mg/l,
Q Trehespceocntitvroelly.substance was added to one bottle at a nominal concentration of 20 mg/l.
The fourth bottle contained only inoculum blank.
Subsequently, the four bottles were made up to 3 litre with Miili—Q water.
Principle
The CO2 produced in each test bottle reacted with the barium hydroxide in the gas
scrubbing bottle and was precipitated out as barium carbonate; the amount of CO2 produced
was determined by titrating the remaining Ba(OH)2 with 0.05 N standardized HCL.
Periodically the CO2 absorber nearest to the test bottle was removed for titration. The
remaining two absorbers were each moved one position in the direction of the test bottle,
and a new absorber filled with 80 ml of 0.025 N Ba(OH)2 (normalised the same day) was
placed at the far end of the series.
Measurements
Titrations were made when necessary (before significant BaCO3 precipitate was evident in
the second trap), approximately every other day for the first 10 days, and thereafter
every fifth day until the 28th day. Phenolphthalein was used as indicator.
On the 26th day, the pH of the four test bottles was measured, and then 1 ml of
concentrated HCl was added to drive off inorganic carbonate. The bottles were aerated
overnight, and samples were removed from each bottle to enable the possibility for
dissolved organic carbon (DOC) analysis. The final titration was made on day 28.
Reference substance
- Reference substance:
- acetic acid, sodium salt
Results and discussion
% Degradationopen allclose all
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 6.4
- Sampling time:
- 9 d
- Remarks on result:
- other: 10 mg/l low
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 10.5
- Sampling time:
- 28 d
- Remarks on result:
- other: 10 mg/l low
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 1.3
- Sampling time:
- 9 d
- Remarks on result:
- other: 20 mg/l high
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 4.1
- Sampling time:
- 28 d
- Remarks on result:
- other: 20 mg/l high
- Details on results:
- THEORETICAL CO2—PRODUCTION
The theoretical CO2—production of UC—136 (TCO2) (brutoformula:(C24H19C12NO2
MW=424) was calculated to be 2.491 mg C02/mg test substance.
The low concentration was 30.3 mg UC—136 in 3 litre test medium and the
high concentration was 60.5 mg UC—136 in 3 litre test medium. Hence, the
theoretical C02—production following complete degradation was 75.5 mg per
3 litre for the low and 150.7 mg per 3 litre for the high concentration.
The positive control contained 60.3 mg sodium acetate (TCO2= 1.073 mg
C02/mg) resulting in a theoretical C02—production of 64.7 mg per 3 litre.
BIODEGRADATION
The relative degradation values calculated from the measurements performed
during the test period revealed no significant degradation of UC—136 at
the high concentration (<10 %) and hardly at the low i.e. 11% .
The control substance degraded with more than 60% within 16 days and 80 %
biodegradation was reached at the end of the test period.
CO2 evolution in the blank reached a total value of 21.7 mg.
MONITORING OF TEMPERATURE AND pH
The temperature of the climate room varied between 18.5 °C and 21.5°C.
The pH values of the different test media were: a. blank: 5.9; b. positive
control: 5.7;c. UC—136 low: 5.9; d. UC—136 high: 4.2.
BOD5 / COD results
- Results with reference substance:
- %deg 40.9 CO2 evolution 9d
%deg 79.5 CO2 evolution 28d
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- valid study
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- Hence, UC—136 appears to be not readily biodegradable under the conditions
used in the present test.
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