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Diss Factsheets

Toxicological information

Skin irritation / corrosion

Currently viewing:

Administrative data

Endpoint:
skin irritation: in vivo
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Justification for type of information:
data is from peer reviewed journals

Data source

Reference
Reference Type:
publication
Title:
The sensitizing capacity of coumarins (III)
Author:
B.M. HAUSEN AND M. Berger
Year:
1989
Bibliographic source:
Contact Dermatitis 21: 141-147,1989

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: As mentioned below
Principles of method if other than guideline:
Skin irritation test was conducted on guinea pigs to determine the irritation potential of 7-Diethylamino-4-methylcoumarin (CAS No: 91-44-1)
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
7-(diethylamino)-4-methyl-2-benzopyrone
EC Number:
202-068-9
EC Name:
7-(diethylamino)-4-methyl-2-benzopyrone
Cas Number:
91-44-1
Molecular formula:
C14H17NO2
IUPAC Name:
7-(diethylamino)-4-methyl-2H-chromen-2-one
Test material form:
other: off white crystalline solid
Details on test material:
- Name of test material (as cited in study report): 7-Diethylamino-4-methylcoumarin
- Molecular formula : C14H17NO2
- Molecular weight :231.2933 g/mol
.- Substance type: organic
Specific details on test material used for the study:
Name of test material (as cited in study report): 4-Methyl-7-diethylaminocoumarin
Molecular formula: C12H13NO2
Molecular weight : 203.24 g/mol
Smiles notation : c12c(ccc(c1)N(CC)CC)c(cc(=O)o2)C
InChl : 1S/C14H17NO2/c1415(52)11671210(3)814(16)1713(12)911/h69H,45H2,13H3
Substance Type: Organic
Physical State: Solid

Test animals

Species:
guinea pig
Strain:
other: Pirbright- White
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Sex: female
- Age at study initiation: No Data available
- Weight at study initiation:280-350 g
- Housing: animals were kept 3 to a cage
- Diet (e.g. ad libitum):altromin ad libitum
- Water (e.g. ad libitum): water ad libitum
- Acclimation period: No Data available

Or Test Tissue
- Source: No Data available
- Type: No Data available
- Acclimation : No Data available
- Housing conditions No Data available

ENVIRONMENTAL CONDITIONS
- Temperature (°C):22-24⁰C
- Humidity (%):50-55%
- Air changes (per hr): No Data available
- Photoperiod (hrs dark / hrs light): Under artificial illumination for 10 hours a day

Test system

Type of coverage:
open
Preparation of test site:
other: clipped or shaved
Vehicle:
other: FCA and Physiological saline
Controls:
not specified
Amount / concentration applied:
10%, 3% and 1 %
Duration of treatment / exposure:
24 hours
Observation period:
24 hours
Number of animals:
10
Details on study design:
TEST SITE
- Area of exposure: clipped or shaved shoulder area (4*6 cm)

OBSERVATION TIME POINTS
(indicate if minutes, hours or days) : The results were read 24 hours after challenge exposure with the test chemical

SCORING SYSTEM:
- Method of calculation:A (+) reaction was considered as threshold of irritation

Results and discussion

In vitro

Other effects / acceptance of results:
no data available

In vivo

Results
Irritation parameter:
overall irritation score
Basis:
mean
Time point:
24 h
Score:
0
Remarks on result:
no indication of irritation
Irritant / corrosive response data:
no irritation observed

Any other information on results incl. tables

Table 1: Results of the study

Test chemical

Concentration

Irritation index

7-diethylamino-4-methyl coumarin (coumarin 1)

10%

0.0

The mean irritation index is the average of 3 readings

Applicant's summary and conclusion

Interpretation of results:
other: not irritating
Conclusions:
The mean irritation index of 7-Diethylamino-4-methylcoumarin was 0.0 after 24 hours.
Hence, the test material 7-Diethylamino-4-methylcoumarin (CAS No: 91-44-1) was considered to be not irritating to guinea pigs
Executive summary:

Skin irritation test was conducted on guinea pigs to determine the irritation potential of 7-Diethylamino-4-methylcoumarin (CAS No: 91-44-1) .

10 female Pirbright-White guinea pigs were treated with an emulsion prepared from FCA and physiological saline but without the test chemical and applied on a semicircular arc on the clipped or shaved shoulder area (4*6 cm) from left to right in such a way that whole emulsion was used up for the animals.1 day before the challenge exposure, teh guinea pigs were treated with 7-Diethylamino-4-methylcoumarin (CAS No: 91-44-1)  by applying different concentrations of the test chemical (10%, 3% and 1%) to the right flank.The results of the challenge exposure were observed after 24 hours. A (+) reaction was considered as threshold of irritation.

The mean irritation index of 7-Diethylamino-4-methylcoumarin was 0.0 after 24 hours.

Hence, the test material 7-Diethylamino-4-methylcoumarin (CAS No: 91-44-1) wasconsidered to be not irritating to guinea pigs