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EC number: 942-358-9 | CAS number: 69207-66-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- April-May 2015
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP, Guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 015
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Trizinc bis[hexacyanidocobaltate] dodecahydrate
- EC Number:
- 942-358-9
- Cas Number:
- 69207-66-5
- Molecular formula:
- Zn3[Co(CN)6]2 ·12H2O
- IUPAC Name:
- Trizinc bis[hexacyanidocobaltate] dodecahydrate
- Test material form:
- solid: particulate/powder
- Remarks:
- migrated information: powder
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- other: Salmonella typhimurium: TA100, TA98, TA97, TA1535, TA102 and the strain E.coli WP2
- Metabolic activation:
- with and without
- Metabolic activation system:
- For the assay with metabolic activation, 0.5 mL of metabolic activation mixture containing 10% of postmitochondrial fraction (S9) together with the bacteria and the test item
- Test concentrations with justification for top dose:
- Main Assay:
Concentrations of test item ranged between 0.05 – 5.0 mg/plate.
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- 2-acetylaminofluorene
- 9-aminoacridine
- 2-nitrofluorene
- sodium azide
- ethylmethanesulphonate
- mitomycin C
Results and discussion
Any other information on results incl. tables
The results indicate that test item Zinc Hexacyanocobaltate dodecahydrate neither produced a statistically significant dose-related increase in the number of revertants nor a statistically significant and reproducible positive response at any of the test points. According to the results obtained in this test system the test item is considered non-mutagenic.
Salmonella typhimurium TA100:
Four independent experiments (including range-finding assays) were performed, two without metabolic activation, two with metabolic activation. The mean levels of spontaneous mutations were within the range of 143-185 revertants/plate. In experiments with and without activation Zinc Hexacyanocobaltate dodecahydrate was tested in concentration range from 0.001 to 5.0 mg/plate. The mutation factor MF did not exceed the value of 2 (MF<2) at any concentration level of the test item. Positive control (sodium azide) induced statistically significant increase (p<0.001) of revertant frequency under experimental conditions without activation (MF = 3.18 and 3.31). The positive response on the treatment with 2AAF showed ability of S9 system in activating of promutagen (MF = 5.8 and 6.14 ).
Salmonella typhimurium TA1535:
Four independent experiments (including range-finding assays) were performed, two without metabolic activation, two with metabolic activation. The mean level of spontaneous mutations was within the range of 18-37 revertants/plate. In experiments without metabolic activation (including assay with preincubation) Zinc Hexacyanocobaltate dodecahydrate was tested in concentration range from 0.001 to 5.0 mg/plate.In experiments without metabolic activation, the level of revertant frequency was not increased in comparison with negative (solvent) control. In experiments with metabolic activation, Zinc Hexacyanocobaltate dodecahydrate was tested in concentration range from 0.01 to 5.0 mg/plate. Test item did not induce any statistically significant increases of revertant frequency in experiments with metabolic activation. Positive control (sodium azide) induced statistically significant (p<0.001) increase of revertant frequency (MF = 22.21, 8.09, 12.36 and 25.35). The ability of S9 system to activate the promutagen was evaluated in each experiment with the use of other Salmonella typhimurium strains (TA100 and TA98 with positive control 2AAF).
Salmonella typhimurium TA97:
Four independent experiments (including range-finding assays) were performed, two without metabolic activation, two with metabolic activation. The mean level of spontaneous mutation was within the range of 140-198 revertants/plate. In experiments without activation Zinc Hexacyanocobaltate dodecahydrate was tested in concentration range from 0.001 to 5.0 mg/plate. The statistically significant increase of mutation frequency against solvent control was not detected at any concentration of the test item. The experiments with metabolic activation were performed in the concentration range from 0.01 to 5.0 mg/plate. The test item did not induce statistically significant increase of revertant frequency in any of the experiments with metabolic activation. Positive control (9-aminoanthracene) induced statistically significant increase of revertant frequency (MF = 4.77, p<0.001, MF = 3.35, p<0.001,MF = 4.71, p<0.001 and MF = 3.34, p<0.001). The efficacy of metabolic activation was confirmed in activation of promutagen 2AAF in experiment with Salmonella typhimurium TA100, TA98 performed under the same conditions.
Salmonella typhimurium TA98:
Four independent experiments (including range-finding assays) were performed, two without metabolic activation, two with metabolic activation. The mean level of spontaneous mutation was within the range of 21- 34 revertants/plate. Zinc Hexacyanocobaltate dodecahydrate was tested in concentrations ranging from 0.01 to 5.0 mg/plate with and without metabolic activation. In experiments without metabolic activation, at each concentration level of the test item, the level of revertant frequency was not increased in comparison with negative (solvent) control. Positive control (2NF) induced statistically significant increase (p<0.001) of revertant frequency (MF=26.3 and 9.33). In experiments with S9 fraction isolated from rat liver after induction with Aroclor or 20-methylcholanthere, Zinc Hexacyanocobaltate dodecahydrate did not induce thestatistically significant increase of revertant frequency at any concentration level. The positive response to the treatment with 2AAF showed the ability of S9 system in activating of promutagen (MF=20.16, p<0.01 and MF=9.83, p<0.001).
Salmonella typhimurium TA102:
Four independent experiments were performed, two without metabolic activation, two with metabolic activation. The mean level of spontaneous mutation was within the range of 184 -224 revertants/plate. In experiments without activation, Zinc Hexacyanocobaltate dodecahydrate was tested in concentration range from 0.01 to 5.0 mg/plate. Within these experiments the level of revertant frequency was not increased in comparison with negative (solvent) control at any concentration level of test item. In the experiment with S9 fraction isolated from rat liver after induction with Aroclor and another with S9 fraction isolated from rat liver after induction with 20-methylcholanthere, Zinc Hexacyanocobaltate dodecahydrate (concentration range from 0.01 to 5.0 mg/plate) did not induce the statistically significant increase of revertant frequency in experiments with metabolic activation. Positive control (mitomycin C) induced statistically significant increase of revertant frequency MF = 2.28, p<0.01, MF = 2.82, p<0.001 and MF = 3.36 , p<0.001). The positive response demonstrating the ability of the S9 system to activate of promutagen was confirmed with positive control 2AAF on other Salmonella strains: TA100 and TA98.
E.coli WP 2:
Two independent experiments were performed, one without metabolic activation, one with metabolic activation. The mean level of spontaneous mutation was within range 15-25 revertants/plate. In experiments without activation, Zinc Hexacyanocobaltate dodecahydrate was tested in concentration range from 0.01 to 5.0 mg/plate. In experiments without metabolic activation, the level of revertant frequency was not increased in comparison with negative (solvent) control. Positive controls (mitomycin C, EMS) induced statistically significant increase of revertant frequency MF = 7.19 and 13.89 respectively, (p<0.001). In the experiment with S9 fraction isolated from rat liver after induction with Aroclor 1254, Zinc Hexacyanocobaltate dodecahydrate (concentration range from 0.01 to 5.0 mg/plate) did not induce the statistically significant increase of revertant frequency in experiments with metabolic activation. The positive response demonstrating the ability of the S9 system to activate of promutagen was confirmed with positive control 2AAF on other Salmonella strains: TA100 and TA98.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
No significant increase in the mutant frequency was observed for the five tester strains of Salmonella typhimurium TA100, TA98, TA97, TA1535, TA102 and one strain of E.coli WP2 after treatment with Zinc Hexacyanocobaltate dodecahydrate in the standard plate incorporation either with or without metabolic activation.
It is concluded that Zinc Hexacyanocobaltate dodecahydrate did not exert mutagenic activity under the conditions of the performed tests. - Executive summary:
Zinc Hexacyanocobaltate dodecahydrate did not produce any significant increases of mutation frequency in these strains up to the maximum dose of 5.0 mg/plate both in the absence and presence of metabolic activation. Zinc Hexacyanocobaltate dodecahydrate did
not exert mutagenic activity under the conditions of the performed tests.
Zinc Hexacyanocobaltate dodecahydrate in accordance with these results is considered to be non-mutagenic in bacterial gene mutation test system.
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