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EC number: 201-121-3 | CAS number: 78-50-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- From Sep. 4, 1985 to Sep. 14, 1985
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- The study was conducted according to a method comparable to OECD guideline 471 and conducted according to GLP. Deviations - strains E.coli WP2 or S. typhimurium TA102 were not included as recommended in the OECD Guideline. It is a read across study, hence maximum reliability rating of 2 assigned according to ECHA guidance.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 985
- Report date:
- 1985
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Remarks:
- Strains E.coli WP2 or S. typhimurium TA102 were not included as recommended in the guideline.
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- A mixture of: hexyldioctylphosphineoxide; dihexyloctylphosphineoxide; trioctylphosphineoxide
- IUPAC Name:
- A mixture of: hexyldioctylphosphineoxide; dihexyloctylphosphineoxide; trioctylphosphineoxide
- Reference substance name:
- A mixture of: hexyldioctylphosphineoxide; dihexyloctylphosphineoxide; trioctylphosphineoxide
- EC Number:
- 403-470-9
- EC Name:
- A mixture of: hexyldioctylphosphineoxide; dihexyloctylphosphineoxide; trioctylphosphineoxide
- IUPAC Name:
- 403-470-9
- Details on test material:
- -Name of test substance (as cited in study report): CYANEX® 923 Extractant
-Lot number: 860-12,13
-Physical state of the test substance: colorless mobile liquid
-Storage conditions of the test substance: room temperature
- Date of receipt: August 28, 1985
Constituent 1
Constituent 2
Method
- Target gene:
- Histidine
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- S. typhimurium TA 1538
- Metabolic activation:
- with and without
- Metabolic activation system:
- S-9 fraction of rat liver homogenate obtained from Aroclor 1254-treated Sprague Dawley rats.
- Test concentrations with justification for top dose:
- 30; 100; 300; 1,000 and 3,000 µg/plate
- Vehicle / solvent:
- - Vehicle used: dimethylsulfoxide (DMSO)
- Justification for choice of solvent/vehicle: the test substance was found to be soluble in DMSO
Controls
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- 2-nitrofluorene
- sodium azide
- other: 2-Anthramine
- Details on test system and experimental conditions:
- Test organism Storage and Maintenance: fresh cultures for mutagenesis testing were prepared by quick thawing a vial of frozen working stock cultures of each tester strain and transferring the culture to 25 mL of OXoid Nutrient Broth #2 and grown for approximately 10 h at 37°C in a New Brunswick Scientific Model G24 Environmental Incubator Shaker. After the 10 h incubation, samples of culture suspensions were diluted 1:4 in distilled water and optical densities were observed at 650 nm using a Beckman Model 35 Spectrophotometer.
Negative and Positive Controls: tester strains were plated in triplicate with the appropriate solvent, both with and without metabolic activation, to obtain background lawn and revertant colony formation to serve as negative solvent controls. In order to validate the integrity of the test system, all tester strains were also run, in triplicate, with known positive response chemicals.
Preliminary Toxicity Screen: the preliminary toxicity screen for the Ames Assay performed without metabolic activation used two of the histidine auxotrophs of Salmonella typhimurium, TA1538 and TAl00. The test compound was prepared to a concentration of 100 mg/mL and five different levels tested for toxicity.
Plate Incorporation Assay: top agar, used as an overlay, was reconstituted into a molten state and supplemented with 0.5 mM histidine and 0.5 mM biotin at a volume of 0.1 Ml/mL of agar, and maintained at 45°C until used. Sterile tubes with kaputs were labeled and placed into a Fisher IsotempR Dry Bath at 45°C. All negative and positive control tubes and plates were done in triplicate. All compound-treated tubes and plates were done in triplicate. Using sterile technique, the following were added to each tube in the following order: 2 mL aliquots of top agar solution, 0.1 mL of tester strain, and 0.1 mL of the appropriate concentration of the test compound. The tubes were vortexed and poured onto minimal glucose plates. The sample was evenly distributed on the plate, and the top agar overlay was allowed to harden. - Evaluation criteria:
- A positive result was defined as a reproducible, dose-related increase in the number of histidine-independent colonies with at least one dose point inducing a mutant frequency value that is two-fold the solvent control value. Significance at the 95% confidence limit was determined by the program developed by Moore and Felton (1983). This program applies a linear regression analysis to the data points and any P value greater than 0.05 is considered significant. In addition, the greater the P value, the higher the probability that the data points fit a linear response. If the solvent control was within the 95% confidence limits of the historical mean for control values and the test chemical produced the highest increase equal to or greater than three times the solvent control value, the test chemical was considered positive. A negative result was defined as the absence of a reproducible increase in the number of histidine independent colonies.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1538
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- RANGE-FINDING/SCREENING STUDIES:
Dose levels in a Preliminary Toxicity Screen were 100; 333; 1,000; 3,333 and 10,000 µg/plate. Strain TA1538 of Salmonella tyhimurium exhibited no inhibition of bacterial lawn growth at the levels tested. A reduced number of revertant colonies were observed in strain TA100 of Salmonella typhimurium at the 3,333 and 10,000 µg/plate. Based upon these findings, the top dose selected for the Plate Incorporation Mutation Assay was 3,000 µg/plate.
Any other information on results incl. tables
Table: Mean revertants per plate
a. Controls
|
|
Mean Spontaneous Revertants/Plate |
||||
Solvent control |
Metabolic activation |
TA1535 |
TA1537 |
TA1538 |
TA98 |
TA100 |
DMSO |
- |
20 (SD.)7 |
16 (SD.)2 |
14 (SD.)6 |
33 (SD.)5 |
212 (SD.)8 |
DMSO |
+ |
15 (SD.)3 |
15 (SD.)6 |
20 (SD.)2 |
54 (SD.)8 |
208 (SD.)18 |
Positive controls |
|
|
|
|
|
|
Sodium Azide |
- |
1050 (SD.)84* |
0 (SD.)0 |
0 (SD.)0 |
0 (SD.)0 |
910 (SD.)51* |
9-Aminoacridine |
- |
0 (SD.)0 |
1364 (SD.)292* |
0 (SD.)0 |
0 (SD.)0 |
0 (SD.)0 |
2-Nitrofluorene |
- |
0 (SD.)70 |
0 (SD.)0 |
177 (SD.)35* |
452 (SD.)45* |
0 (SD.)0 |
2-Anthramine |
+ |
171 (SD.)4* |
158 (SD.)21* |
586 (SD.)76* |
1296 (SD.)194* |
1470 (SD.)12* |
* Positive response
b. Test substance
|
|
Mean Total Revertant Colonies/Plate |
||||
Dose level (Test substance) µg/plate |
Metabolic activation |
TA1535 |
TA1537 |
TA1538 |
TA98 |
TA100 |
30 |
- |
22 (SD.) 8 |
15 (SD.) 3 |
16 (SD.)9 |
49 (SD.)7 |
208 (SD.)40 |
100 |
- |
19 (SD.) 1 |
12 (SD.) 6 |
19 (SD.)5 |
38 (SD.)9 |
199 (SD.)11 |
300 |
- |
15 (SD.) 6 |
9 (SD.) 4 |
14(SD.)6 |
37 (SD.)6 |
191 (SD.)12 |
1000 |
- |
21(SD.) 10 |
6 (SD.) 2 |
15 (SD.)6 |
35 (SD.)5 |
166 (SD.)18 |
3000 |
- |
22 (SD.) 2 |
8 (SD.) 3 |
12 (SD.)5 |
35 (SD.)5 |
147 (SD.)38 |
30 |
+ |
12 (SD.)3 |
15 (SD.)2 |
27(SD.)11 |
52 (SD.)6 |
149 (SD.)18 |
100 |
+ |
11 (SD.)6 |
16 (SD.)9 |
30 (SD.)9 |
57 (SD.)7 |
141 (SD.)18 |
300 |
+ |
12 (SD.)4 |
16 (SD.)2 |
26 (SD.)6 |
56 (SD.)6 |
137 (SD.)8 |
1000 |
+ |
13 (SD.)1 |
9 (SD.)2 |
26 (SD.)2 |
46 (SD.)7 |
129 (SD.)3 |
3000 |
+ |
11 (SD.)8 |
9 (SD.)3 |
23 (SD.)6 |
45 (SD.)7 |
129 (SD.)26 |
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
Under the study conditions, the read across substance was considered non-mutagenic in this Salmonella typhimurium reverse mutation assay (Barfknecht TR, 1985). - Executive summary:
A study was conducted to evaluate the mutagenicity of the read across substance ‘A mixture of: hexyldioctylphosphineoxide; dihexyloctylphosphineoxide; trioctylphosphineoxide’ using a method similar to OECD Guideline 471, in compliance with GLP. The test substance was subjected to a preliminary toxicity screen at dose levels of 100, 333, 1,000, 3,333 and 10,000 µg/plate. Based upon the findings, the top dose selected for the plate incorporation mutation assay was 3,000 µg/plate. Test substance was evaluated in strains TA1535, TA1537, TA1538, TA98 and TA100 of Salmonella typhimurium with and without metabolic activation preparation at doses of 30, 100, 300, 1,000 and 3,000 µg/plate. The results were negative in all groups. All positive and solvent controls used in the evaluation of the test substance induced mean mutant frequency values which were within the acceptable range of mean historical data. Under the study conditions, the read across substance was considered non-mutagenic in this Salmonella typhimurium reverse mutation assay (Barfknecht TR, 1985).
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