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EC number: 274-660-5 | CAS number: 70528-90-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Experiment start date: 06 December 1979; Experiment end date: 04 February 1980; Study completion date: 07 March 1980.
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 980
- Report date:
- 1980
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Deviations:
- not specified
- GLP compliance:
- no
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- 5-[(4-chloro-2-nitrophenyl)azo]-1-ethyl-1,2-dihydro-6-hydroxy-4-methyl-2-oxonicotinonitrile
- EC Number:
- 274-660-5
- EC Name:
- 5-[(4-chloro-2-nitrophenyl)azo]-1-ethyl-1,2-dihydro-6-hydroxy-4-methyl-2-oxonicotinonitrile
- Cas Number:
- 70528-90-4
- Molecular formula:
- C15H12ClN5O4
- IUPAC Name:
- 5-[(4-chloro-2-nitrophenyl)diazenyl]-1-ethyl-6-hydroxy-4-methyl-2-oxo-1,2-dihydropyridine-3-carbonitrile
- Test material form:
- other: solid
Constituent 1
- Specific details on test material used for the study:
- Name: FAT 36091/D
Purity: 50.2 %
Test animals
- Species:
- rat
- Strain:
- other: Tif:RAIf (SPF) strain
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- Healthy random bred rats of the Tif: RAIf (SPF) strain (7 to 8 weeks old) raised on the premises were used for these experiments. They were kept at a room temperature of 22 + 2 °C, at a relative humidity of 55 + 10 % and on a 10 hours light cycle day. They received ad libitum rat food - NAFAG, Gossau SG - and water. During the treatment and observation period the animals were housed in groups of 5 in Macrolon cages (type 3), individually marked with picric acid.
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- CMC (carboxymethyl cellulose)
- Remarks:
- 2% (w/v) in dist. water.
- Details on oral exposure:
- VEHICLE
Volume (ml/kg body-weight): 20
The test item was suspended to achieve the corresponding dosage level. Before treatment the suspension was homogeneously dispersed with an Ultra-Turrax and during treatment it was kept stable with a magnetic stirrer. - Doses:
- 5000, 7000, 8000 and 10000 mg/kg.
- No. of animals per sex per dose:
- 5 males and 5 females
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Bodyweights were recorded immediately prior to dosing (control weights) and at 7 and 14 days
- Necropsy of survivors performed: no
- Other examinations performed: clinical signs, body weight.
Treatment and observations
Animals fasted overnight were treated by single oral intubation. Physical condition and rate of deaths were monitored throughout the whole observation period. - Statistics:
- LD50 inclusing 95 % confidence limits are calculated by the logit model.
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- ca. 8 000 mg/kg bw
- Based on:
- test mat.
- Mortality:
- At 5000 mg/kg: No deaths observed.
At 7000 mg/kg: 1 male died
At 8000 mg/kg: 2 male and 3 female died
At 10000 mg/kg: 3 male and 2 female died - Clinical signs:
- other: - Sedation, dyspnoea, ruffled fur, diarrhoea, body position curved were observed at 5000 mg/kg. The surviving animals recovered within 7 days. - Sedation, dyspnoea, ruffled fur, diarrhoea, body position curved were observed at 7000 mg/kg. The surviving an
- Gross pathology:
- No substance related gross organ changes were seen.
- Other findings:
- No data
Any other information on results incl. tables
Body weight changes:
Dose (mg/kg) | |||||
5000 | 7000 | 8000 | |||
Day 1 Male | Mean body weight/SD (g) | 208/18.4 | 185/14.2 | 188/9.0 | 188/11.4 |
Day 1 female | Mean body weight/SD (g) | 180/8.3 | 180/5.9 | 174/7.3 | 173/4.6 |
Day 7 Male | Mean body weight/SD (g) | 243/23.4 | 239/16.7 | 220/10.0 | 247/11.3 |
Day 7 female | Mean body weight/SD (g) | 205/13.6 | 198/8.6 | 193/10.6 | 195/.0. |
Day 14 Male | Mean body weight/SD (g) | 290/25.5 | 280/21.9 | 261/12.9 | 293/20.5 |
Day 14 female | Mean body weight/SD (g) | 237/27.8 | 212/3.8 | 223/15.6 | 218/8.1 |
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- The acute oral LD50 of FAT 36091/D in rats of both sexes observed over a period of 14 days is approximately 8000 mg/kg.
- Executive summary:
A study was conducted according to method similar or equivalent to OECD TG 401 to determine the acute oral toxicity of the test on rats via oral route. 5 males and 5 females were given following doses; 5000, 7000, 8000, 10000 mg/kg bw. Physical condition and rate of deaths were monitored throughout the whole observation period. Doses of 5000 to 10000 mg/kg bw was given by oral gavage. Before administration, the test item was prepared in CMC (Carboxymethyl cellulose).
Mortality;
At 5000 mg/kg: No deaths observed.
At 7000 mg/kg: 1 male died
At 8000 mg/kg: 2 male and 3 female died
At 10000 mg/kg: 3 male and 2 female died
Symptoms were observed in all dose levels: Sedation, dyspnoea, ruffled fur, diarrhoea, body position curved. The surviving animals recovered within 7 to 8 days. The acute oral LD50 of FAT 36091/D in rats of both sexes observed over a period of 14 days is approximately 8000 mg/kg.
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