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Environmental fate & pathways

Biodegradation in water: screening tests

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Description of key information

The substance is readily biodegradable, with 78.9 % degradation observed in 28 days and meeting the 10-day window.

Key value for chemical safety assessment

Biodegradation in water:
readily biodegradable

Additional information

A study was performed to determine the potential for biodegradation of the substance in water by the CO2 evolution method based on OECD TG 301B (CO2 evolution). An activated sewage sludge was used. The substance and a reference substance were added to the inoculum at a concentration of 10 mg C/L. A blank control containing inoculum only was run alongside the substance vessels. Test vessels were incubated aerobically in the dark for a period of 28 days at ca. 22°C. Approximately 60% CO2 was evolved by Day 10, with 78.9% biodegradation was observed in 28 days thereby indicating the test material is "readily biodegradable".

The reference substance, sodium benzoate, was considered readily biodegradable under the OECD definition (> 60% CO2 production in 28 days and within a 10-day window of reaching 10% CO2 production). Thus the microbial inoculum for the study was acceptable.