2(5H)-Furanone, 5-hydroxy-4-methyl-

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP-guideline study including analytcal dose verification

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2000

Report date:

2000

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

5-hydroxy-4-methyl-2,5-dihydrofuran-2-one

EC Number:

609-869-6

Cas Number:

40834-42-2

Molecular formula:

C5 H6 O3

IUPAC Name:

5-hydroxy-4-methyl-2,5-dihydrofuran-2-one

Details on test material:

- Name of test material (as cited in study report): Hydroxybutenolid
- Molecular formula (if other than submission substance): C5H6O3
- Physical state: solid
- Analytical purity: 92.7 %
- Impurities (identity and concentrations): 0.12 % Water
- Product No.: 068039
- Expiry date: no limit

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

The concentration control analyses were performed with test solutions of the following nominal concentrations (mg/I) of the test substance: control, 3.13, 25 and 100 mg/L
At the start of the test (after 0 h) samples without and at the end of the test (after 48 h) samples from vessels with daphnids were analysed .

Test solutions

Vehicle:

no

Details on test solutions:

The test substance was stirred in M4 medium for about 10 minutes at 20 +/- 2°C . The nominal concentration of the stock solution was 100 mg/L. By diluting this stock solution with M4 medium the following nominal concentrations (mg/I) were prepared : 3.13, 6.25, 12.5, 25, 50, 100 mg/L

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Source:The clone of Daphnia magna STRAUS 1820 used was supplied by the Institut National de Récherche Chimique Appliquée, France, in 1978. From this date on this clone was cultured and bred continuously in the Laboratory Experimental Toxicology and Ecology of BASF AG in Ludwigshafen.
- Age at study initiation (mean and range, SD): 2 - 24 h
- Feeding during test: no

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Test temperature:

20.3 - 20.5 °C

pH:

7.2 - 8.9

Dissolved oxygen:

8.1 - 8.6

Nominal and measured concentrations:

Nominal: 0, 3.13, 6.25, 12.5, 25, 50, 100 mg/L
Measured: within the levels of +/- 20 % of nominal

Details on test conditions:

TEST SYSTEM
- Test vessel: Test tubes (glass) with flat bottom (nominal volume 20 ml)
- Type (delete if not applicable): open
- fill volume: 10 ml
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 0.5 animals/ml

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: M4-medium

OTHER TEST CONDITIONS
- Adjustment of pH: none
- Photoperiod: 16: 8 h day night rhythm
- Light intensity: about 2 - 7 µE/m2*s) at wave length of 400 - 700 nm

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : swimming ability

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

48.3 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility