3-chlorobutan-2-one

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2003

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: OECD Guideline Study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2003

Report date:

2003

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

3-chlorobutan-2-one

EC Number:

223-834-9

EC Name:

3-chlorobutan-2-one

Cas Number:

4091-39-8

Molecular formula:

C4H7ClO

IUPAC Name:

3-chlorobutan-2-one

Constituent 2

Reference substance name:

3-Chloro-2-butanone

IUPAC Name:

3-Chloro-2-butanone

Test material form:

other: colourless liquid

Details on test material:

Purity: > 98% (GC)

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

One aliquot from the freshly prepared test media of all test concentrations was
taken at the start of the test before filling the test vessels. For the determination of
the stability of the test item under the test conditions, respectively the maintenance
of the test item concentrations during the test period, one combined aliquot
was collected from the test media of all 4 exposure vessels of all test concentrations
at the end of the test (after 48 hours).
Storage of the samples:
All samples were stored at -20°C immediately after sampling and were kept
stored to enable additional analyses. After delivery of the final report all samples
were discarded. In a first step the 48-hour samples were analysed. No analysis of
any further samples was carried out if the results revealed correctly prepared
exposure media.

Test solutions

Vehicle:

no

Details on test solutions:

The measured actual concentrations of the 48-hour samples were within +/- 20%
of the nominal concentrations. Hence, it can be assumed that no test
substance was lost. Furthermore the analytical results indicate a homogenous
distribution in the exposure media. Hence, an exposure to the test substance took
place. No analysis of any further samples was carried out as the results revealed
correctly prepared exposure media.
Nominal concentrations were employed for the assessment as the actual concentrations
were within +/- 20% of the nominal concentrations.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Daphnia magna STRAUSS
Acclimatisation at least 1 week
Age 6 - 24 hours, laboratory bred
Feed log-phase Scenedesmus subspicatus/
86.81 SAG';
a small amount of sewage (aerated stage), only
during the holding period
Holding and dilution water
reconstituted, aerated fully demineralised water
the resalting was carried out by addition of
CaCI2: 2.00 mmol/L
MgS04: 0.50 mmollL
NaHC03: 0.77 mmol/L
KCI: 0.077 mmol/L
ratio Ca/Mg ions: 4: 1
ratio Na/K ions: 10 : 1
total alkalinity: 0.8 mmol/L
total hardness: 250 mg CaC03/L

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

no data

Test conditions

Hardness:

total hardness: 250 mg CaC03/L

Test temperature:

air-conditioned chamber, 20°C +/- 1°C

pH:

7.9 +/- 0.3

Dissolved oxygen:

> 80% of maximum saturation

Salinity:

Holding and dilution water
reconstituted, aerated fully demineralised water
the resalting was carried out by addition of
CaCI2: 2.00 mmol/L
MgS04: 0.50 mmollL
NaHC03: 0.77 mmol/L
KCI: 0.077 mmol/L
ratio Ca/Mg ions: 4: 1
ratio Na/K ions: 10 : 1
total alkalinity: 0.8 mmol/L
total hardness: 250 mg CaC03/L

Nominal and measured concentrations:

Nominal concentrations:

Details on test conditions:

Number of animals per test concentration: 20, divided into 4 groups of 5 animals each, 5 animals per vessel
Breeding vessels: all glass vessels with watch glass dishes diameter: 190 mm, height: 90 mm, volume: 2 L
Feeding: no feeding during the test
Illumination: 16 hours light 1 8 hours darkness
Test vessels: all glass vessels diameter: 38 mm, height: 60 mm, volume: 50 mL

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

ca. 0.215 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

ca. 2.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 0.8 - 11.3

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

ca. 10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

Those animals not able to swim within 15 seconds after gentle agitation of the
test vessels were considered to be immobile. Observations were made at 24 and
48 h after the start of the study.

Reported statistics and error estimates:

The EC50 (50% immobilisation of the Daphnia after 24 and 48 hours) was calcu-
lated by linear regression analysis employing probit analysis (Probit Program
Version 1.5, U.S. Environmental Protection Agency, 1992).
The NOEC, EC0 and EC100 values were taken directly from the raw data.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The EC50 (48 h) was determined to be 2.4 mg/L in a static OECD 202 study with Daphnia magna.

Executive summary:

The purpose of this study was to evaluate the influence of the test substance on

the mobility respectively survival of daphnia magna. Young Daphnia were exposed

in a static test for 48 hours to the test medium, which contained the test substance

up to 100 mg/L in test water.

The test medium of the highest test concentration of 100 mg/L exposure medium

was prepared by mixing 100 mg test item into 1 liter test water and by intense

stirring for approximately 10 minutes. The solution obtained was used as stock

solution for lower concentrated test media.

The nominal concentrations tested were 0.215, 0.464, 1.00, 2.15, 4.64, 10.0

and 100 mg/L. Additionally, two controls were tested in parallel.

Aliquots were taken at the beginning and end of the exposure period for GCanalysis.

The GC-analysis revealed correctly prepared exposure media.

The analytical results obtained were within :t 20% of the nominal concentrations.

The reported biological results are related to nominal concentrations.

Concentrations tested (nominal concentration)

0 (control), 2.215, 0.464, 1.00,2.15,4.64, and 10.0 mg/L

48 h NOEC: 0.215 mg/L

48 h EC0: 0.215 mg/L

48 h EC50: 2.4 mg/L (0.8 - 11.3 mg/L)

48 h EC100: 10 mg/L