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EC number: 266-006-2 | CAS number: 65996-73-8 The wetted particulate matter recovered by wet collection techniques, including pollution abatement equipment, for the recovery of materials such as iron sinter dust, blast furnace dust, steelmaking dust, and mill scale.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 8.12.2009-1.2.2010
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP gudeline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
- Report date:
- 2010
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.13/14 (Mutagenicity - Reverse Mutation Test Using Bacteria)
- Deviations:
- yes
- Remarks:
- In case of the strain TA 98, frozen stock culture after expiration of minimum shelf life (two years) was used for experiments - a deviation from SOP. The bacteria were tested for their properties (fenotype confirmation, response to positive controls). All
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Slimes and Sludges, blast furnace and steelmaking
- EC Number:
- 266-006-2
- EC Name:
- Slimes and Sludges, blast furnace and steelmaking
- Cas Number:
- 65996-73-8
- Molecular formula:
- not applicable
- IUPAC Name:
- Sludges steelmaking
- Details on test material:
- - Physical state: solid
- Composition of test material, percentage of components: Fe (total) 54.29% (mainly as oxides), CaO 10.17%, Zn 5.25%, MgO 4.32%, C 2.06%, SiO2 1.64%, Mn 0.69%, K2O 0.126%, Na2O 0.239%, Al2O3 0.16%
- Lot/batch No.: 21.10.2009
- Expiration date of the lot/batch: unlimited
- Stability under test conditions: unlimited
- Storage condition of test material: stored in PE container at room temperature
Constituent 1
Method
- Target gene:
- gene for synthesis histidine or tryptophan
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Additional strain / cell type characteristics:
- other: histidine (Salmonella) and tryptophan (Escherichia) dependents
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 fraction of rat liver homogenate and mixture of cofactors
- Test concentrations with justification for top dose:
- With regard to poor solubility the test substance was dosed (50, 150, 500, 1500, 5000 µg/plate).
- Vehicle / solvent:
- water for injections (Ardeapharma, batch No. 0203200809, exp. 08/2011)
Controlsopen allclose all
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- Remarks:
- AS
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- Remarks:
- AAc
Migrated to IUCLID6: hydrochloride monohydrate
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- other: 4-nitro-1,2-phenylenediamine (CASRN: 99-56-9)
- Remarks:
- NPD
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- other: 2-aminofluorene (CASRN: 153-78-6)
- Remarks:
- AF
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- other: 2-aminoanthracene (CASRN: 613-13-8)
- Remarks:
- AA
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- other: N-methyl-N´-nitro-N-nitrosoguanidin (CASRN: 70-25-7)
- Remarks:
- MNNG
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation)
NUMBER OF REPLICATIONS: 3 - Evaluation criteria:
- The main criterion for evaluation of results was modified two-fold increase rule which is compatible with the application of statistical methods (Dunkel V. C., Chu K.C.: Evaluation of methods for analysis of microbial mutagenicity assays, in The Predictive Value of Short-Term Screening Tests in Carcinogenicity Evaluation, Elsevier North-Holland Biomedical Press, 231 - 240, 1980; Claxton L. D. et al.: Guide for the Salmonella typhimurium/mammalian microsome tests for bacterial mutagenicity, Mutat. Res. 189, 83 - 91, 1987). After this rule the result is positive, if a reproducible dose-response effect occurs and/or a doubling of the ratio Rt/Rc is reached.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not determined
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not determined
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not determined
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not determined
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not determined
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Table 1: The effect of the test substance
Salmonella typhimurium TA 100 | ||||||
doses | S9 | mean ± sd | Rt/Rc | S9 | mean ± sd | Rt/Rc |
(µg/plate) | (µl) | (µl) | ||||
experiment I without metabolic activation | experiment I with metabolic activation | |||||
spont. rev | 0 | 142±12 | - | 30 | 160±15 | - |
H2O | 0 | 158±5 | - | 30 | 153±13 | - |
50 | 0 | 159±7 | 1.0 | 30 | 166±15 | 1.1 |
150 | 0 | 161±7 | 1.0 | 30 | 175±8 | 1.1 |
500 | 0 | 174±8 | 1.1 | 30 | 158±8 | 1.0 |
1500 | 0 | 179±1 | 1.1 | 30 | 154±12 | 1.0 |
5000 | 0 | 159±19 | 1.0 | 30 | 158±8 | 1.0 |
AS/2-AF | 0 | 476±4 | 3.0 | 20 | 1543±13 | 10.1 |
experiment II without metabolic activation | experiment II with metabolic activation | |||||
spont. rev | 0 | 131±7 | - | 30 | 134±6 | - |
H2O | 0 | 133±7 | - | 30 | 141±12 | - |
50 | 0 | 136±5 | 1.0 | 30 | 138±18 | 1.0 |
150 | 0 | 140±13 | 1.1 | 30 | 142±11 | 1.0 |
500 | 0 | 136±8 | 1.0 | 30 | 151±18 | 1.1 |
1500 | 0 | 135±4 | 1.0 | 30 | 162±14 | 1.1 |
5000 | 0 | 138±14 | 1.0 | 30 | 166±2 | 1.2 |
AS/2-AF | 0 | 434±33 | 3.3 | 20 | 787±33 | 5.6 |
Salmonella typhimurium TA 1535 | ||||||
doses | S9 | mean ± sd | Rt/Rc | S9 | mean ± sd | Rt/Rc |
(µg/plate) | (µl) | (µl) | ||||
experiment I without metabolic activation | experiment I with metabolic activation | |||||
spont. rev | 0 | 24± 2 | - | 30 | 18± 2 | - |
H2O | 0 | 26± 3 | - | 30 | 23± 4 | - |
50 | 0 | 20± 1 | 0.8 | 30 | 21± 2 | 0.9 |
150 | 0 | 24± 4 | 0.9 | 30 | 20± 0 | 0.9 |
500 | 0 | 31± 2 | 1.2 | 30 | 27± 3 | 1.2 |
1500 | 0 | 26± 1 | 1.0 | 30 | 20± 3 | 0.8 |
5000 | 0 | 25± 3 | 1.0 | 30 | 23± 6 | 1.0 |
AS/2-AA | 0 | 522±31 | 20.1 | 20 | 234± 7 | 10.0 |
experiment II without metabolic activation | experiment II with metabolic activation | |||||
spont. rev | 0 | 27± 2 | - | 30 | 25± 2 | - |
H2O | 0 | 29± 3 | - | 30 | 22± 4 | - |
50 | 0 | 30± 4 | 1.0 | 30 | 22± 3 | 1.0 |
150 | 0 | 25± 1 | 0.8 | 30 | 23± 0 | 1.1 |
500 | 0 | 26± 2 | 0.9 | 30 | 27± 5 | 1.2 |
1500 | 0 | 30± 4 | 1.0 | 30 | 26± 4 | 1.2 |
5000 | 0 | 29± 4 | 1.0 | 30 | 20± 6 | 0.9 |
AS/2-AA | 0 | 435± 2 | 14.8 | 20 | 198±10 | 9.0 |
Salmonella typhimurium TA 98 | ||||||
doses | S9 | mean ± sd | Rt/Rc | S9 | mean ± sd | Rt/Rc |
(µg/plate) | (µl) | (µl) | ||||
experiment I without metabolic activation | experiment I with metabolic activation | |||||
spont. rev | 0 | 29± 0 | - | 30 | 30± 4 | - |
H2O | 0 | 33± 5 | - | 30 | 30± 7 | - |
50 | 0 | 35± 3 | 1.1 | 30 | 35± 3 | 1.2 |
150 | 0 | 33± 5 | 1.0 | 30 | 26± 2 | 0.9 |
500 | 0 | 34± 4 | 1.0 | 30 | 26± 4 | 0.9 |
1500 | 0 | 33± 3 | 1.0 | 30 | 26± 4 | 0.9 |
5000 | 0 | 38± 5 | 1.1 | 30 | 26± 3 | 0.9 |
AS/2-AF | 0 | 1520±91 | 46.0 | 20 | 1576±45 | 52.5 |
experiment II without metabolic activation | experiment II with metabolic activation | |||||
spont. rev | 0 | 26± 1 | - | 30 | 40± 3 | - |
H2O | 0 | 27± 3 | - | 30 | 33± 4 | - |
50 | 0 | 26± 4 | 1.0 | 30 | 36± 3 | 1.1 |
150 | 0 | 25± 5 | 1.0 | 30 | 28± 4 | 0.8 |
500 | 0 | 29± 2 | 1.1 | 30 | 34± 8 | 1.0 |
1500 | 0 | 25± 1 | 0.9 | 30 | 31± 4 | 0.9 |
5000 | 0 | 24± 3 | 0.9 | 30 | 32± 3 | 1.0 |
AS/2-AF | 0 | 1382±22 | 51.8 | 20 | 1351±22 | 40.5 |
Salmonella typhimurium TA 1537 | ||||||
doses | S9 | mean ± sd | Rt/Rc | S9 | mean ± sd | Rt/Rc |
(µg/plate) | (µl) | (µl) | ||||
experiment I without metabolic activation | experiment I with metabolic activation | |||||
spont. rev | 0 | 13± 3 | - | 30 | 15± 4 | - |
H2O | 0 | 13± 3 | - | 30 | 16± 1 | - |
50 | 0 | 12± 2 | 0.9 | 30 | 17± 2 | 1.1 |
150 | 0 | 14± 2 | 1.1 | 30 | 16± 2 | 1.0 |
500 | 0 | 15± 2 | 1.2 | 30 | 17± 1 | 1.1 |
1500 | 0 | 11± 0 | 0.9 | 30 | 16± 2 | 1.0 |
5000 | 0 | 10± 2 | 0.8 | 30 | 13± 2 | 0.8 |
AS/2-AA | 0 | 861±77 | 67.9 | 20 | 224±23 | 14.0 |
experiment II without metabolic activation | experiment II with metabolic activation | |||||
spont. rev | 0 | 11± 3 | - | 30 | 12± 2 | - |
H2O | 0 | 10± 1 | - | 30 | 10± 2 | - |
50 | 0 | 11± 2 | 1.1 | 30 | 10± 2 | 1.0 |
150 | 0 | 10± 2 | 1.0 | 30 | 12± 1 | 1.2 |
500 | 0 | 9± 0 | 0.9 | 30 | 12± 1 | 1.2 |
1500 | 0 | 9± 3 | 1.2 | 30 | 10± 1 | 1.0 |
5000 | 0 | 11± 0 | 1.1 | 30 | 8± 1 | 0.8 |
AS/2-AA | 0 | 1127±46 | 112.7 | 20 | 147± 3 | 14.7 |
Escherichia coli WP2 uvrA | ||||||
doses | S9 | mean ± sd | Rt/Rc | S9 | mean ± sd | Rt/Rc |
(µg/plate) | (µl) | (µl) | ||||
experiment I without metabolic activation | experiment I with metabolic activation | |||||
spont. rev. | 0 | 39± 5 | - | 100 | 32± 3 | - |
H2O | 0 | 32± 5 | - | 100 | 31± 1 | - |
50 | 0 | 33± 2 | 1.0 | 100 | 34± 8 | 1.1 |
150 | 0 | 35± 6 | 1.1 | 100 | 32± 4 | 1.0 |
500 | 0 | 34± 1 | 1.1 | 100 | 35± 4 | 1.1 |
1500 | 0 | 34± 3 | 1.1 | 100 | 35± 3 | 1.1 |
5000 | 0 | 33± 5 | 1.0 | 100 | 34± 4 | 1.1 |
MNNG/2-AA | 0 | 449± 1 | 14.0 | 100 | 162±12 | 5.2 |
experiment II without metabolic activation | experiment II with metabolic activation | |||||
spont. rev | 0 | 41± 5 | - | 100 | 44± 2 | - |
H2O | 0 | 35± 3 | - | 100 | 42± 1 | - |
50 | 0 | 39± 6 | 1.1 | 100 | 38± 4 | 0.9 |
150 | 0 | 44± 4 | 1.2 | 100 | 35± 1 | 0.8 |
500 | 0 | 39± 3 | 1.1 | 100 | 44± 1 | 1.0 |
1500 | 0 | 38± 5 | 1.1 | 100 | 45± 4 | 1.1 |
5000 | 0 | 44± 7 | 1.3 | 100 | 47± 2 | 1.1 |
MNNG/2-AA | 0 | 463± 3 | 13.2 | 100 | 325±19 | 7.7 |
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results: negative
Under the experimental design, the test substance Slimes and Sludges, blast furnace and steelmaking was nonmutagenic for all the Salmonella typhimurium as well as Escherichia coli strains both in experiments without as well as with metabolic activation. - Executive summary:
Test substance Slimes and Sludges, blast furnace and steelmaking was assayed for the mutagenicity by the Bacterial Reverse Mutation Test. The test was performed according to EU method B.13/14 Mutagenicity – Reverse mutation test using bacteria, which is analogous to the OECD Test Guideline No. 471.
Four indicator Salmonella typhimurium strains TA 98, TA 100, TA 1535 and TA 1537 and one indicator Escherichia coli WP2 uvrA strain were used. The test substance was suspended in water for injections and assayed in doses of 50-5000 µg which were applied to plates in volume of 0.1 ml. Two series of experiments were performed with each strain - without metabolic activation and with a supernatant of rat liver and a mixture of cofactors.
In the arrangement given above, the test substance Slimes and Sludges, blast furnace and steelmaking was nonmutagenic for all the used bacterial strains with as well as without metabolic activation.
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