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EC number: 200-193-3 | CAS number: 54-11-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms, other
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2014-08-18 to 2014-09-17
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: The inhibition control in standardised biodegradation tests is considered to be an acceptable alternative for the standard OECD TG 209 result
- Justification for type of information:
- The inhibition control in standardised biodegradation tests is considered to be an acceptable alternative for the standard OECD TG 209 result.
- Qualifier:
- according to guideline
- Guideline:
- other: EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- no
- Principles of method if other than guideline:
- The toxicity to micro-organisms has been evaluated in the ready biodegradability test. This result will be used to evaluate the hazard to micro-organisms in an sewage treatment plant.
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- SAMPLING
- Sampling frequency: ten samples were taken within the 28 days
- Sampling method: From each front scrubber flask, ten samples were taken in order to determine the emitted CO2 (on days 0, 2, 4, 7, 9, 11, 14, 18, 23, and 29). The sample volume was 1 mL.
On day 28, 5 mL 2 M HCl were added to each test flask in order to drive off dissolved CO2. On day 29, samples from both scrubber flasks were taken. - Details on test solutions:
- - Stock solutions:
Solution a
Potassium dihydrogen phosphate (KH2PO4) 8.5 g
Di-potassium hydrogen phosphate (K2HPO4) 21.75 g
Di-sodium hydrogen phosphate dihydrate (Na2HPO4*2H2O) 33.4 g
Ammonium chloride (NH4Cl) 0.5 g
H2O demin. ad 1000 mL
The pH was adjusted to 7.4 +/- 0.1.
Solution b
Calcium chloride dihydrate (CaCl2*2H2O) 36.4 g
H2O demin. ad 1000 mL
Solution c
Magnesium sulfate heptahydrate (MgSO4*7H2O) 22.5 g
H2O demin. ad 1000 mL
Solution d
Iron(III) chloride hexahydrate (FeCl3*6H2O) 0.25 g
Di-sodium ethylenediamine tetraacetate dihydrate (Na2EDTA*2H2O) 0.4 g
H2O demin. ad 1000 mL
- Test medium:
Solution a 10 mL
Solution b 1 mL
Solution c 1 mL
Solution d 1 mL
H2O demin. ad 1000 mL
The medium was freshly prepared.
CONTROL AND BLANK SYSTEM
- Inoculum blank: 2, containing mineral medium and inoculum
- Abiotic sterile control: 1, containing test item, mineral medium and HgCl2
- Toxicity control: 1, containing test item, positive control, mineral medium and in-oculum - Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- - source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): The sludge was taken from the activation basin of the ESN (Stadtentsorgung Neustadt) sewage treatment plant.
- Pretreatment: The sludge was filtered, washed with tap water twice, then washed with and resuspended in the test medium. It was then aerated until use. The dry matter was determined with 4220 mg suspended solids/litre. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 28 d
- Test temperature:
- 21.6-23.5
- pH:
- 7.3 at the end of the test.
- Reference substance (positive control):
- yes
- Remarks:
- Aniline
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 27 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- other: respiration rate
- Details on results:
- • The test item nicotine is considered as “readily biodegradable“.
• The degree of biodegradation reached 71% after 28 days.
• The 10-day-window began on day 3, at its end, 72% were reached, surpassing the pass level of 60% given in the OECD guideline.
• Abiotic degradation was not observed.
As degradation in the toxicity flask was 70% after 14 days, the test item can be stated as "not toxic towards the inoculum in a concentration of 27 mg/L. - Results with reference substance (positive control):
- See table at "Any other information on results incl. tables". Degradation of positive control was >60% within 7 days.
- Validity criteria fulfilled:
- yes
- Remarks:
- All validity criteria were met. Degradation of the positive control was 71 % after seven days.
- Conclusions:
- Degradation behaviour of positive control and toxicity control was normal. Abiotic degradation was not observed. Both replicates of the test item showed very good correspondence.
The validity criteria were met and the criterion for ready biodegradability was fulfilled.
If degradation in the toxicity flask is below 25% after 14 days, the test item can be considered as toxic towards the inoculum. As degradation in the toxicity flask was 70% after 14 days, the test item can be stated as “not toxic towards the inoculum in a concentration of 27 mg/L”.
No observations were made which might cause doubts concerning the validity of the study outcome.
The result of the test can be considered valid. - Executive summary:
The test item nicotine was tested using a concentration of at least 20 mg organic carbon/L (corresponding to 27.0 mg nicotine/L) in test medium following OECD 301B and EU-Method C.4-C.
Aniline was chosen as positive control.
Activated sludge was used as inoculum (concentration in the test 25.0 mg dry matter/L). The test was left running for 28 days.
All validity criteria were met. Degradation of the positive control was 71% after seven days.
The following data were determined for the test item nicotine:
10-day-window: day 3 – 13
degradation at the end of 10-day-window: 72%degradation at the end of the test: 71%
pass level following guideline: 60% at the end of 10-day-window
No inhibition of the degradation of the reference substance was observed in the toxicity control of this test in the presence of 27 mg/L.
Reference
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Description of key information
One key study of high quality is available (Klimisch 1). The study is GLP compliant and appropriate for risk assessment, therefore no further testing is needed.
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 27 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.