Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 204-857-3 | CAS number: 127-68-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- Experimental test result performed using standard test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Principles of method if other than guideline:
- Short term toxicity of test chemical to aquatic algae was performed according to the OECD Guideline 201 (Alga, Growth Inhibition Test) in a static system.
- GLP compliance:
- not specified
- Analytical monitoring:
- not specified
- Vehicle:
- not specified
- Details on test solutions:
- The solution of white pearl 100 mg/l was prepared by dissolving the substance in OECD growth medium.
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name:
- Strain: 86.81 SAG
- Source (laboratory, culture collection): Institute of botany of the ASCR
- Initial biomass concentration: 5x10(3) cells /ml
- Method of cultivation: No data available
ACCLIMATION - No data available
- Acclimation period:
- Culturing media and conditions (same as test or not):
- Any deformed or abnormal cells observed: - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Remarks on exposure duration:
- ±1 hour
- Test temperature:
- 23±2°C
- pH:
- Sample: 8.1 (changed to 7.8 during test)
Control: 8.3 (changed to 9.1 during test) - Nominal and measured concentrations:
- Test conducted at 100 mg/l nominal concentration
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 100 ml Glass vessel
- Type (delete if not applicable): closed (with air permeable stopper)
- Sample volume: 30 ml
- Initial cells density: 5x10(3) cells/ml
- No. of vessels per concentration (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: Continuous
- Light intensity and quality: 6000-8000 lx
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: microscope with counting chamber Cyrus I or electronic particle counter.
- Other: ErC50 was calculated using non-linear regression by the software Prism 4.0 - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate (K2Cr2O7)
- Key result
- Duration:
- 72 h
- Dose descriptor:
- other: IC0
- Remarks:
- %
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: No inhibition were observed at 100 mg/l.
- Results with reference substance (positive control):
- Results with reference substance valid
- EC50: 0.65 mg/L - Reported statistics and error estimates:
- The differences in means of control and sample were estimated by the t-test for independent groups at a 95 % confidence level, all individual replicates were used (STATISTICA CZ – data analysis software system, version 9.0, StatSoft, Inc.). Statistically significant differences are for p < 0.05.
- Validity criteria fulfilled:
- yes
- Conclusions:
- After the exposure of chemical with algae Desmodesmus subspicatus, no inhibition were observed after the 72 hrs. And the IC0% was determine at 100 mg/l.
- Executive summary:
Aim of this study was to assess the short term toxicity of test chemical to aquatic algae and cyanobacteria. Study was performed according to the OECD Guideline 201 (Alga, Growth Inhibition Test) in a static system for the total exposure period of 72 hrs. The solution of white pearl 100 mg/l was prepared by dissolving the substance in OECD growth medium. Test conducted at limit concentration of 100 mg/l. Strain 86.81 SAG were used in the study. 5x10(3) cells/ml were added in 100 ml test vessel. Effects on growth rate were observed for 72 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, inhibitory concentration IC was calculated. The differences in means of control and sample were estimated by the t-test for independent groups at a 95 % confidence level, all individual replicates were used (STATISTICA CZ – data analysis software system, version 9.0, StatSoft, Inc.). Statistically significant differences are for p < 0.05. After the exposure of chemical with algae Desmodesmus subspicatus, no inhibition were observed. And the IC0% was determine at 100 mg/l. As no effect were observed at limit dose 100 mg/l thus it was concluded that the EC50 was > 100 mg/. Based on the no effect on the growth of algae, it was concluded that the chemical was nontoxic and not classified as per the CLP classification criteria.
Reference
Description of key information
Aim of this study was to assess the short term toxicity of test chemical to aquatic algae and cyanobacteria. Study was performed according to the OECD Guideline 201 (Alga, Growth Inhibition Test) in a static system for the total exposure period of 72 hrs. The solution of white pearl 100 mg/l was prepared by dissolving the substance in OECD growth medium. Test conducted at limit concentration of 100 mg/l. Strain 86.81 SAG were used in the study. 5x10(3) cells/ml were added in 100 ml test vessel. Effects on growth rate were observed for 72 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, inhibitory concentration IC was calculated. The differences in means of control and sample were estimated by the t-test for independent groups at a 95 % confidence level, all individual replicates were used (STATISTICA CZ – data analysis software system, version 9.0, StatSoft, Inc.). Statistically significant differences are for p < 0.05. After the exposure of chemical with algae Desmodesmus subspicatus, no inhibition were observed. And the IC0% was determine at 100 mg/l. As no effect were observed at limit dose 100 mg/l thus it was concluded that the EC50 was > 100 mg/. Based on the no effect on the growth of algae, it was concluded that the chemical was nontoxic and not classified as per the CLP classification criteria.
Key value for chemical safety assessment
- EC10 or NOEC for freshwater algae:
- 100 mg/L
Additional information
Aim of this study was to assess the short term toxicity of test chemical to aquatic algae and cyanobacteria. Study was performed according to the OECD Guideline 201 (Alga, Growth Inhibition Test) in a static system for the total exposure period of 72 hrs. The solution of white pearl 100 mg/l was prepared by dissolving the substance in OECD growth medium. Test conducted at limit concentration of 100 mg/l. Strain 86.81 SAG were used in the study. 5x10(3) cells/ml were added in 100 ml test vessel. Effects on growth rate were observed for 72 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, inhibitory concentration IC was calculated. The differences in means of control and sample were estimated by the t-test for independent groups at a 95 % confidence level, all individual replicates were used (STATISTICA CZ – data analysis software system, version 9.0, StatSoft, Inc.). Statistically significant differences are for p < 0.05. After the exposure of chemical with algae Desmodesmus subspicatus, no inhibition were observed. And the IC0% was determine at 100 mg/l. As no effect were observed at limit dose 100 mg/l thus it was concluded that the EC50 was > 100 mg/. Based on the no effect on the growth of algae, it was concluded that the chemical was nontoxic and not classified as per the CLP classification criteria.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.