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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Endpoint:
toxicity to microorganisms
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: The study was well documented and meets generally accepted scientific principles, but was not conducted in compliance with GLP.

Data source

Reference
Reference Type:
publication
Title:
Comparison of the toxicity thresholds of water pollutants to bacteria, algae, and protozoa in the cell multiplication inhibition test.
Author:
Bringmann G, Kuhn R
Year:
1980
Bibliographic source:
Water Research 14(3): 231-241.

Materials and methods

Test guideline
Qualifier:
no guideline followed
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
not specified

Test organisms

Test organisms (species):
Entosiphon sulcatum

Study design

Test type:
other: Cell multiplication inhibition test
Water media type:
freshwater
Total exposure duration:
72 h
Remarks on exposure duration:
Incubation period

Test conditions

Test temperature:
25 degC
Details on test conditions:
TEST SYSTEM
- Test vessel: 300 ml Erlenmeyer flasks

- Test vessel content: 8ml culture liquid. 8ml stock solution I, 2ml united preliminary protozoan cultures (approx. impulses 15000/ml)I, and 10ml each of bacterial suspension (or 10ml each of saline for the dilution series not inoculated) and 2ml of suspension of inactivated bacteria from a preliminary bacterial culture having a known extinction value.

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Double distilled water

OTHER TEST CONDITIONS
- Adjustment of pH: Pollutant solution adjusted to pH6.9 using the minimum volume of hydrochloric acid or caustic potash.

EFFECT PARAMETERS MEASURED (with observation intervals if applicable)
- After incubating, add 10% of a 1.1%NaNO3 solution in double distilled water filtered through a membrane filter (pore size 0.2um) to each falsk and determine the number of protozoa by means of a cell counter.


TEST CONCENTRATIONS
- Spacing factor for test concentrations: Four parallel dilution series, 1:2 to 1:2E+14 v/v. One series inoculated, one not.
Reference substance (positive control):
not required
Remarks:
not applicable

Results and discussion

Effect concentrations
Duration:
72 h
Dose descriptor:
other: TT or EC3
Effect conc.:
44 mg/L
Basis for effect:
other: cell multiplication inhibition

Applicant's summary and conclusion

Conclusions:
A toxicity threshold (TT) value for bacteria (Entosiphon sulcatum) of 44 mg/l was determined in a reliable study conducted according to generally accepted scientific principles.