(2S)-pentan-2-ol

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2002-07-16 until 2002-09-26

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Good laboratory practice guideline study (OECD).

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2008

Report date:

2008

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Reference

Name:

Unnamed

Type:

Constituent

Details on test material:

- Name of test material (as cited in study report): (S)-Pentan-2-ol
- Substance type: intermediate
- Physical state: liquid
- Stability under test conditions: pure: stable until 2008-09-30,
in Water: stable under test conditions
- Storage condition of test material: room temperature in the dark

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
not applicable

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, non-adapted

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): sewage plant Darmstadt, Germany
- Preparation of inoculum for exposure: Washing by centrifugation and supernatant phase was decanted. Solid material was resuspended and again centrifuged. This procedure was repeated twice.
- Pretreatment: not applicable
- Concentration of sludge: 1.5 g dry material per litre

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

100 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

TEST CONDITIONS
- Composition of medium: a) buffer KH2PO4, K2HPO4, Na2HPO4, NH4Cl ; b) Mg2SO4 x 7 H2O ; c) CaCl2 x 2 H2O ; FeCl3 x 6 H2O
- Test temperature: 22 °C
- pH: 7.6
- pH adjusted:no
- Suspended solids concentration: 30 mg per litre
- Continuous darkness: yes

TEST SYSTEM
- Number of culture flasks/concentration: 2
- Measuring equipment: nanometric (BSB/BOD-Sensor-System Aqualytic)
- Test performed in closed vessels due to significant volatility of test substance: no
- Test performed in open system: yes


CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: yes (test water)
- Toxicity control: yes (Sodium benzoate and test substance)
- Other: procedure control (Sodium benzoate)

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

% Degradation

Parameter:

% degradation (O2 consumption)

Value:

>= 93 - <= 94

Sampling time:

28 d

Details on results:

Toxicity control, procedure control, inorganic control worked as expected.

BOD5 / COD results

Results with reference substance:

96% biodegradation after 28 days

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

The substance is readily biodegradable.

Executive summary:

The test substance was readily biodegradable in a manometric respiratory test performed according to OECD guideline 301F showing a degradation of 93 and 94% after 28 days.