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EC number: 247-722-4 | CAS number: 26471-62-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: inhalation
Administrative data
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1977-06-16 to 1977-07-30
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 980
- Report date:
- 1980
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 403 (Acute Inhalation Toxicity)
- Deviations:
- yes
- Remarks:
- The duration of exposure was 1 h instead of at least 4 h. 4 animals per sex instead of 5. The humidity was low (4 %) instead of 30 - 70 %.
- GLP compliance:
- no
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- m-tolylidene diisocyanate
- EC Number:
- 247-722-4
- EC Name:
- m-tolylidene diisocyanate
- Cas Number:
- 26471-62-5
- Molecular formula:
- C9H6N2O2
- IUPAC Name:
- 2,4-diisocyanato-1-methylbenzene, 2,6-diisocyanato-1-methylbenzene
- Details on test material:
- - Name of test material (as cited in study report): Toluene diisocyanate (80:20 mixture of the 2,4- and 2,6- isomers)
- Isomers composition: 79.6 % (2,4 isomer)
- Lot/batch No.: ABH2146
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: ABH2146
OTHER SPECIFICS:
- Isomers composition: 79.6 % (2,4 isomer)
Test animals
- Species:
- rat
- Strain:
- other: Alderly Park
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Age at study initiation: 6 - 10 weeks
- Weight at study initiation: 150 - 300 g
- Fasting period before study: none
- Housing: 4 rats/cage
- Diet : ad libitum except during the exposure, Oakes Powder 'O' diet
- Water : ad libitum, except during the exposure
Administration / exposure
- Route of administration:
- inhalation: vapour
- Type of inhalation exposure:
- whole body
- Vehicle:
- air
- Details on inhalation exposure:
- GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus: heated tube
- Exposure chamber volume: a 17 L glass dessicator was used as an exposure chamber (there were no divisions in order to minimise deposition).
- Method of conditioning air: The TDI was dispended into a syringe atomizer by a palmer slow injection apparatus (CF palmer limited). The entrained vapour passed through a heated glass tube (about 120 °C), mixed with diluting air and led into the animal exposure chamber. All air was dried to less than 4 % relative humidity.
- Temperature, humidity, pressure in air chamber: no data, 4 %, no data
TEST ATMOSPHERE
- Brief description of analytical method used: The test atmosphere is drawn throught a glass sintered bubbler containing absolute alcohol. The Isocyanate (Urethane derivative) is subsequently analysed by Liquid Chromatography.
- Samples taken from breathing zone: yes
- Analytical verification of test atmosphere concentrations:
- yes
- Remarks:
- A sample of air from the breathing zone of the rats was drawn through two sintered glass absorbers containing 10 mL of absolute alcohol at a rate of 1.25 L/min for 5 min mid-way through each exposure. The trapped isocyanates were then analysed by HPLC.
- Duration of exposure:
- 1 h
- Concentrations:
- 0, 17.4, 25.16, 43.2, 83.9, and 267.1 ppm (0.13, 0.18, 0.31, 0.61, and 1.93 mg/L)
- No. of animals per sex per dose:
- 4
- Control animals:
- yes
- Details on study design:
- - Duration of observation period following administration: at least 14 days
- Frequency of observations and weighing: The behaviour of the animals was recorded throughout the exposure. Then, examination and weighing during the following fourteen days were performed at approximately 2 days intervals.
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight, lung weight
Results and discussion
Effect levels
- Key result
- Sex:
- male/female
- Dose descriptor:
- LC50
- Effect level:
- 66 ppm
- Based on:
- test mat.
- 95% CL:
- > 31 - < 141
- Exp. duration:
- 1 h
- Remarks on result:
- other: (LC50 = 0.48 mg/L) There was no significant difference between the LC50 of male and female rats.
- Mortality:
- Yes, 11/18 deaths occurred on day 1 or 2. For more details see table 1 in field "Remarks on results including tables and figures".
- Clinical signs:
- other: At 17.4 ppm: after exposure, all animals were subdued and wheezing. Animals were hunched and blue. 2 females lost weight over 10 days. 1 female died on day 15 and 1 was sacrificed in extremis. At 25.16 ppm: after exposure, all animals were wheezing
- Body weight:
- All of the animals exposed which survived the first 36 hours after exposure lost weight during the first 4 days of the observation period. Some continued to lose weight and either died or were sacrificed.
- Gross pathology:
- See remarks.
- Other findings:
- - Potential target organs: lung
- Organ weights: The lung weights were increased indicating oedema.
- Other observations: Necropsy findings:
(1) At 17.4 ppm, 1 female died on day 15 had dark patches on all lobes of lungs; the animal sacrificed on day 15 had pale inflated lungs with red 2 lobes of right lung; lung weight was high.
(2) At 25.16 ppm: 1 female dying on day 2 had multiple dark red foci on all lung lobes, 2 dark red lobes; others sacrificed at end of study had red foci on lungs and consolidated lobes; 3 males had pale inflated lungs; lung weights were increased.
(3) At 43.2 ppm: animals dying had inflated lungs with dark patches full of fluid; at necropsy, lung weights increased and all lungs were pale, inflated with 2 animals showing dark patches.
(4) At 83.9 ppm: animals dying on study had red foci in lungs and animals surviving had inflated and pale lungs except for red areas of consolidation, lung weights were increased.
(5) At 267.1 ppm: animals dying on day 1 had fluid in lung and thorax, animals dying after day 1 had inflated, red lungs; single survivor had pale enlarged lungs with red spots on all lobes, enlarged adrenals and gray kidneys.
Any other information on results incl. tables
Table 1: Concentrations, time of death and mortality per animals treated
Conc. (ppm) |
Isomer ratio 2,4:2,6 |
Mortality (# dead/total) | Time range of deaths (days) | ||
Male | Female | Combined | |||
17.4 | 3.13:1 | 0/4 | 1/4 | 1/8 | day 15 |
25.16 | 2.53:1 | 1/4 | 3/4 | 4/8 | (2-19) |
43.2 | 4.1:1 | 2/4 | 1/4 | 3/8 | (2-11) |
83.9 | 3.13:1 | 1/4 | 2/4 | 3/8 | (2-4) |
267.1 | 2.27:1 | 4/4 | 3/4 | 7/8 | (1-2) |
controls | - | 0/4 | 0/4 | 0/8 | - |
Applicant's summary and conclusion
- Interpretation of results:
- Category 2 based on GHS criteria
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