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Diss Factsheets
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EC number: 200-589-6 | CAS number: 64-67-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Developmental toxicity / teratogenicity
Administrative data
- Endpoint:
- developmental toxicity
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 3 (not reliable)
- Rationale for reliability incl. deficiencies:
- other: The study was well conducted but the treatment was performed by ip injection. This route of administration is hardly extrapolable to the normal conditions of use of a chemical.
Cross-reference
- Reason / purpose for cross-reference:
- reference to other study
Data source
Reference
- Reference Type:
- publication
- Title:
- Developmental Anomalies: Mutational Zygote Exposure
- Author:
- Generoso WM, Rutledge JC, Aronson J
- Year:
- 1 990
- Bibliographic source:
- Banbury report 34: 311-319
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Exposure of the dams by ip injection at at 4 days before mating or 1, 6, 9 or 25 hours after mating. after mating. Uterine analysis done on the 17th day.
- GLP compliance:
- no
- Limit test:
- no
Test material
- Reference substance name:
- Diethyl sulphate
- EC Number:
- 200-589-6
- EC Name:
- Diethyl sulphate
- Cas Number:
- 64-67-5
- Molecular formula:
- C4H10O4S
- IUPAC Name:
- diethyl sulfate
- Details on test material:
- Supplier Aldrich Chemical Co
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- other: (C3H/R1 X 101/R1)F1
Administration / exposure
- Route of administration:
- intraperitoneal
- Vehicle:
- DMSO
- Analytical verification of doses or concentrations:
- not specified
- Details on mating procedure:
- Mating for 30 min only, beginning at the time when the newly ovulated eggs were in the ampulla of the oviduct, thus synchronizing the time of fertilization.
- Duration of treatment / exposure:
- 1 injection
- Frequency of treatment:
- not relevant (1 injection)
- Duration of test:
- up to the 17th day of gestation
Doses / concentrations
- Remarks:
- Doses / Concentrations:
150 mg/kg
Basis:
no data
- No. of animals per sex per dose:
- between 17 and 54 females were mated. Between 17 and 43 were pregnant.
- Control animals:
- yes, concurrent vehicle
Results and discussion
Results: maternal animals
Effect levels (maternal animals)
- Dose descriptor:
- dose level:
- Effect level:
- 150 mg/kg bw (total dose)
- Based on:
- no data
- Basis for effect level:
- other: developmental toxicity
Results (fetuses)
- Details on embryotoxic / teratogenic effects:
- Embryotoxic / teratogenic effects:yes
Fetal abnormalities
- Abnormalities:
- not specified
Overall developmental toxicity
- Developmental effects observed:
- not specified
Any other information on results incl. tables
Treatment |
Resorption bodies (%) |
Mid- and late-gestation deaths (%) |
Live fetuses examined |
Live fetuses with defect (%) |
Control |
5 |
1.5 |
3436 |
1.3 |
DES |
24 |
37 |
157 |
24 |
fetal anomalies (% of the number of fetuses examined)
Hydropia: 6.4
Abdominal wall: 3.2
Limb and/or tail: 12.7
Eyes: 2.5
Exencephaly: 1.9
Applicant's summary and conclusion
- Conclusions:
- DES was toxic for the zygote and induced fetal malformations.
- Executive summary:
The developmental and teratogenic effects of DES were studied in mouse after ip injection of 150 mg/kg. Dams were exposed early after mating (between 1 and 9 hours). The effects were oberved at the 17th gestation day.
DES induced stillbirths and anomalies of the fetuses.
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