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Diss Factsheets
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EC number: 701-492-6 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study meets the criteria for Klimisch code 1 because it followed an internationally recognised method (EPA OTS 797.1300) and was carried out to Good Laboratory Practice.
- Qualifier:
- according to guideline
- Guideline:
- EPA OTS 797.1300 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)
- Deviations:
- no
- Remarks:
- None reported
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- Total organic carbon (TOC) measurements of initial (0 hours) test solutions and at test termination (48 hours).
- Vehicle:
- no
- Details on test solutions:
- The 100, 300 and 1000 mg/L nominal loadings were prepared using sterilised filtered well water. Individual water accommodation fractions were prepared by stirring the vessel for 24 hours with a Teflon coated magnetic stir bar and removing the water phase for the toxicity test.
Dilution water was filtered well water collected at Hampton, New Hamshire. Water was adjusted to a hardness of 176 mg/L, passed through activated carbon and then an ultraviolet steriliser. Water was stored in a polyethylene tank where it was aerated.
No undissolved test material was reported on the surface of the test vessels during the entire toxicity test. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Waterflea
- Source: In-house laboratory culture
- Age at study initiation (mean and range, SD): Juvenile (<24 hours old) - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- Not reported
- Hardness:
- 176 mg CaCO3/L
- Test temperature:
- 20.4 to 20.9°C
- pH:
- 7.2 to 8.1
- Dissolved oxygen:
- 7.9 to 8.7mg/L
- Salinity:
- Not relevant
- Nominal and measured concentrations:
- Control, 100, 300 and 1000 mg/L WAF.
TOC levels were 2.8-3.5 mg/L in the control, 2.8-3.6 mg/L at 100 mg/L WAF, 3.0-3.7 mg/L at 300 mg/L WAF and 2.7-3.4 mg/L at 1000 mg/L WAF loading. TOC levels were not considered to be indicative of actual test material concentrations and results are therefore based on nominal loading rates. - Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 mL glass beaker containing 200 mL of test solution, loosely covered to reduce the entry of dust
- No. of organisms per vessel: Ten
- No. of vessels per concentration (replicates): Two
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Filtered well water collected at Hampton, New Hampshire
- Total organic carbon: 2 mg/L
- Alkalinity: pH 7.2 to 8.1
- Conductivity: 860 to 880 µmhos/cm
OTHER TEST CONDITIONS
- Photoperiod: 16-hours light per day
- Light intensity: 20 µEin/m²
EFFECT PARAMETERS MEASURED: Immobilisation
TEST CONCENTRATIONS
- Test concentrations: Control, 100, 300 and 1000 mg/L nomial WAF - Reference substance (positive control):
- no
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: WAF loading rate
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: WAF loading rate
- Basis for effect:
- mobility
- Details on results:
- Control immobilisation did not exceed the test validity criterion (i.e. <10% immobilisation in the controls).
At 24-hours, no immobilised or dead organisms were observed in the control or treatments. At 48-hours, 5, 0, 20 and 5% immobilisation were reported for control, 100, 300 and 1000 mg/L, respectively. - Reported statistics and error estimates:
- The statistical analysis of survival data is not warranted.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The test substance was not toxic to daphnids at loading rates up to 1000 mg/L and the EC50 is considered to be >1000mg/l.
- Executive summary:
The 48–hour acute toxicity of benzenesulphonic acid, C14-24 branched / linear alkyl derivative, calcium salt to Waterfleas (Daphnia magna) was studied under static conditions. Daphnids were exposed to control and test chemical at nominal concentrations of 100, 300 and 1000 mg/L WAF for 48 hours. Mortality/immobilisation and sublethal effects were observed daily.
This study is classified as acceptable and satisfies the guideline requirements for an acute toxicity study with freshwater invertebrates.
Results Synopsis
Test Organism Age: Neonates (<24 hours old)
Test Type: Static
Percent survival/unaffected organisms was 95% in the control, 100% at 100 mg/l, 80% at 300 mg/l and 95% at 1000 mg/l.
48 hour Effective Loading Rate (EL50) = >1000 mg/L
Reference
Description of key information
The toxcity of the substance has been measured experimentally with freshwater Daphnia. Due to the extremely low water solubility all studies have been conducted using Water Accomodated Fractions and completed as limit tests.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 1 000 mg/L
Additional information
The EC50 in freshwater Daphnia is considered to be >1000 mg/l. The substance is, therefore, considered not toxic to Daphnia magna. Nevertheless, adequate controls will be in place during production and use to ensure that the substance is not released to the environment.
The toxicity of the substance has not been assessed using marine invertebrate species, but it is considered likely to be similar to freshwater species.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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