2,5-Dichloro-4-(1,1,2,3,3,3-hexafluoropropoxy)phenylamine

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1994

Report date:

1994

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

Deviations:

yes

Remarks:

only one CO2 scrubber was used

GLP compliance:

yes (incl. QA statement)

Test material

Reference

Name:

Unnamed

Type:

Constituent

Details on test material:

- Theoretical carbon content: 62,86 %
- Identification code: CA 944 A
- Batch No.: P.810012
- Purity: 93,2 %
- Received: 1993-08-05

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Details on inoculum:

Activated sludge was collected from the sewage treatment plant of CH-4153 Reinach on 15/11/93.
The pH after collection was 7.0.
The preparation was carried out according to the method described in the guideline.

Duration of test (contact time):

29 d

Initial test substance concentration

Initial test substance concentration 1

Initial conc.:

27.3 mg/L

Based on:

ThOD/L

Initial test substance concentration 2

Initial conc.:

28.5 mg/L

Based on:

ThOD/L

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

CO2 evolution

Details on study design:

DESING AND PROCEDURE
- Vessels: 2 Liter flasks (dark brown glass) equipped with gas inlet and magnetic stirrer
- Air: 25 ml/min carbon dioxide free air
- CO2 scubbers: approx 250 ml scrubbers with gas inlet tubes equipped with sintered glass tips containing 200 ml 0,05 N sodium hydroxide

Only one CO2 scrubber was used per test buttle. Theoretically, a solution of 0,05 M NaOH is sufficient to trap at least two times more CO2 than the maximum ThCO2 wich can evolve in each test bottle (including CO2 possibly evolved from the bacteria e.g., endogenous respiration). Moreover, experimentally it was confirmed that no measurable CO2 carry over has ever occured with the scubbers used.

SET UP OF FLASKS
- Flasks 1 and 2: test substance and inoculum
- Flasks 3 and 4: test medium and inoculum (inoculum blank)
- Flask 5: reference substance and inoculum (procedure control)
- Flask 6: reference substance, test substance and inoculum (control of toxicity and inhibitoren of the bacteria's activity by the test substance)

DURATION AND SAMPLING
The test duration was 29 days. During this time the evolved CO2 trapped by the 0,05 N NaOH solution was measured at 0, 2, 6, 10, 14, 16, 21, 28 and 29 days. The last measurement was performed after acidification of the medium with 1 ml of concentrated HCl.

Reference substance

Reference substance:

other: Sodium benzoate

Results and discussion

% Degradation

Parameter:

% degradation (CO2 evolution)

Value:

0

Sampling time:

29 d

Applicant's summary and conclusion

Interpretation of results:

under test conditions no biodegradation observed