Adiponitrile

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Study period:

from 20DEC1979 08FEB1982

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Method is described, no numerical value resulted from the testing performed. Quality issues are reported on the study.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1982

Report date:

1982

Materials and methods

Principles of method if other than guideline:

Five groups of five male and five female Sprague-Dawley rats per group were each exposed for six hours to different atmospheric concentrations of adiponitrile (ADN). Analytical concentrations of ADN in the chamber ranged from 0.79 to 2.18 mg/l in air.

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Adiponitrile

EC Number:

203-896-3

EC Name:

Adiponitrile

Cas Number:

111-69-3

Molecular formula:

C6H8N2

IUPAC Name:

hexanedinitrile

Details on test material:

- Name of test material (as cited in study report): Adiponitrile
- Physical state: Clear liquid
- Analytical purity: 99.3%

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

Male and female Sprague-Dawley rats were obtained from the Charles River Breeding Laboratories. Test animals were held and observed in quarantine for seven days prior to release for the study. At the time of exposure , aninals were 42-54 days of age with males weighing 200-280 grams and females 150-202 grams. Each rat was uniquely identified by eartag and bar-coded cage card.
Animals were individually housed in suspended stainless steel wire mesh cages (23 cm x 23 cm x 21 cm) and given Purina Laboratory Standard Rodent Chow® (5001)2 and tap water (St. Louis City, MO) ad libitum except during the exposure period. Animal rooms were routinely maintained at 70-740F and 35-65% relative humidity with a 12-hour light/dark cycle.

Administration / exposure

Route of administration:

inhalation: aerosol

Type of inhalation exposure:

whole body

Duration of exposure:

6 h

Concentrations:

0.79 mg/L
1.00 mg/L
1.41 mg/L
1.56 mg/L
2.18 mg/L

No. of animals per sex per dose:

5 males and 5 females per dose.

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LC50

Effect level:

> 2.18 mg/L air (analytical)

Exp. duration:

6 h

Applicant's summary and conclusion

Conclusions:

The presence of chromorhinorrhea, hemorrage of the lung and gray appearance of the lung are indications that ADN is a respiratory irritant. Only six male rats out of 50 died during the study with concentrations from 0.79 to 2.18 mg/L, making it impossible to calculate a LC50.

Executive summary:

LC50 > 2.18 mg/L. Test substance is a respiratory irritant (used for classification)