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EC number: 200-002-3 | CAS number: 50-01-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to soil microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to soil microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Principles of method if other than guideline:
- Degradation of C-14 guanidinium chloride in field soil samples was followed by trapping and counting of radioactivity (14-CO2). Test were made with sterile soil (no degradation), different concentrations of test substance, different additions carbon source (cellulose, glucose) and nitrogen.
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- 1µCi carbon-14 guanidine chloride (Amersham, 22 mCi/mMole) + unlabeled ganidine chloride
- Analytical monitoring:
- yes
- Details on sampling:
- 1 x per day
- Test organisms (inoculum):
- soil
- Total exposure duration:
- 23 d
- Test temperature:
- 20 °C
- Moisture:
- 60% soil mosture holding capacity
- Details on test conditions:
- The mineralization of guanidinium carbon was measured in the incubation and trapping system described by Marrinucci and Bartha (1979). Triplicate test flasks received 1 microCi carbon-14 guanidine chloride (Amersham, 22 mCi/mMole), sufficient distilled water to achieve 60% soil moisture holding capacity, and the equivalent of 20 g dry weight soil. Chemical additions including unlabeled guanidine chloride and the inhibitors mercuric chloride and sodium azide were made in conjunction with the moisture ad- justment. The organic carbon sources, cellulose and glucose, were added as sterile dry solids (4% by weight) and were mixed to homogeneity with a sterile glass rod.
- Reference substance (positive control):
- yes
- Remarks:
- Guanidinium chloride
- Key result
- Duration:
- 23 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 400 mg/kg soil ww
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- Guanidinium ion
- Basis for effect:
- other: Reduction of mineralization of Guanidinium Carbon
- Key result
- Duration:
- 23 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 636 mg/kg soil ww
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- Guanidine chloride
- Basis for effect:
- other: Reduction of mineralization of Guanidinium Carbon
- Details on results:
- Mineralization rates showed no tendency to decrease at any concetnration ( tested: 10, 25, 50, 100, 200, 400 mg/kg) until most of the Guanidinium was degraded.
- Validity criteria fulfilled:
- not applicable
- Conclusions:
- For loading rates of 10 - 400 mg Guanidine /kg soil ww (15.9 - 636 mg Guanidine chloride /kg soil ww) mineralisation rates of Guanidine carbon to Carbon dioxide showed not tendency to decrease at any concentration.
- Executive summary:
In a non guideline test with natural soil microorganism, degradation rates for mineralisation of 14C radiolabeled guanidine carbon were determined. Quantification was made by determination of trapped 14C labelled carbon dioxide. For loading rates of 10 - 400 mg Guanidine /kg soil ww (15.9 - 636 mg Guanidine chloride /kg soil ww) mineralisation rates of Guanidine carbon to Carbon dioxide showed not tendency to decrease at any concentration.
Reference
Shown in Table are the effects of two carbon sources and three major nitrogenous components in nitroguanidine wastestreams on guanidinium carbon mineralization. Both glucose and to a much lesser extent its natural polymer cellulose enhanced 24 hour mineralization rates: production of radiolabeled carbon dioxide was nearly four times the unamended control in the presence of glucose and was increased by twenty percent in the presence of cellulose.
The wastewater components nitroguanidine, ammonium, and nitrate showed only a slight tendency to inhibit guanidinium mineralization whether alone at equimolar concentrations to guanidinium or in concert. Previous results have demonstrated that environmental microorganisms in surface water can mineralize guanidinium carbon slowly as a sole substrate and more rapidly in the presence of metabolizable carbon (Mitchell, 1987). While the relative contribution of such populations to the mineralization of the cation in soil can not be assessed in the present study, results with the added carbon sources suggest that the latter process can also be stimulated in soil.
Table Effects of Carbon and Nitrogen Compounds on the Mineralization of Guanidinium Carbon in Duffield Soil
Sample nM Mineralized/24Hours (1xsigma) %Control (1xsigma)
Control(i) 634 (4) 100 (<1)
Carbon added as(ii):
Cellulose 776 (26) 122.4 (4)
Glucose 2481 (273) 391.4 (43)
Nitrogen added as (ii):
Ammonium 579 (47) 91.3 (7)
Nitrate 596 (8) 94 (1.3)
Nitroguanidine 542 (22) 85.5 (3.5)
Composite 578 (44) 91.7 (7)
i. Triplicate flasks each contained 1 µCi and 20.9 µM guanidinium at a concentration of 100 mg/Kg soil.
ii. Carbon and nitrogen compounds were 4% (w/w) and 20.9 µM respectively.
Description of key information
For loading rates of 10 - 400 mg Guanidine /kg soil ww (15.9 - 636 mg Guanidine chloride /kg soil ww) mineralisation rates of Guanidine carbon to Carbon dioxide showed not tendency to decrease at any concentration.
Key value for chemical safety assessment
- Long-term EC10 or NOEC for soil microorganisms:
- 636 mg/kg soil dw
Additional information
In a non guideline test with natural soil microorganism, degradation rates for mineralisation of 14C radiolabeled guanidine carbon were determined. Quantification was made by determination of trapped 14C labelled carbon dioxide. For loading rates of 10 - 400 mg Guanidine /kg soil ww (15.9 - 636 mg Guanidine chloride /kg soil ww) mineralisation rates of Guanidine carbon to Carbon dioxide showed not tendency to decrease at any concentration.
(Remark: key value for chemical safety assesment NOEC 636 mg/kg is wet weight; pick list doesn't allow to choose ww but dw)
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