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EC number: 210-180-4 | CAS number: 609-15-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 15 June 2010 - 09 July 2010
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: This study has been performed according to OECD and/or EC guidelines and according to GLP principles.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
- Report date:
- 2010
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- ISO 6341 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea))
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: Guidance document on aquatic toxicity testing of difficult substances and mixtures, OECD series on testing and assessment number 23, December 14, 2000.
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Ethyl 2-chloroacetoacetate
- EC Number:
- 210-180-4
- EC Name:
- Ethyl 2-chloroacetoacetate
- Cas Number:
- 609-15-4
- Molecular formula:
- C6H9ClO3
- IUPAC Name:
- ethyl 2-chloro-3-oxobutanoate
- Reference substance name:
- Chloracetessigester
- IUPAC Name:
- Chloracetessigester
- Details on test material:
- - Name of test material (as cited in study report): Chloracetessigester
- Substance type: Clear slightly yellowish liquid
- Physical state: Liquid
- Analytical purity: 93.9%
- Lot/batch No.: 001008
- Expiration date of the lot/batch: 26 July 2010
- Stability under storage conditions: Stable
- Storage condition of test material: At room temperature in the dark
-Stability in water: Unknown
-Solubility in water: Yes, 17 g/L
- Density: 1.18 g/cm3 (20°C)
Constituent 1
Constituent 2
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- During the final test duplicate samples for possible analysis were taken from all test concentrations and the control according to the schedule below.
Frequency: at t=0 h and t=48 h
Volume: 4 ml from the approximate centre of the test vessels
Storage: Not applicable, samples were analysed on the day of sampling.
At the end of the exposure period, the replicates were pooled at each concentration before sampling.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- The batch of Chloracetessigester tested was a clear slightly yellowish liquid with a purity of 93.9% and the substance appeared to be completely soluble in test medium at the concentrations tested.
Preparation of test solutions started with the highest test concentration of 100 mg/l applying a 15 to 18-minute period of magnetic stirring. The lower test concentrations were prepared by subsequent dilutions of the highest concentration in test medium. During the final test dilutions were prepared from a stirring solution and all test vessels used were incubated for 1 day with the respective test solutions as to prevent any possible adsorption to glass ware. After the pre-incubation period vessels were filled with freshly prepared solutions. All final test solutions were visually clear and colourless.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna (Crustacea, Cladocera) (Straus, 1820), at least third generation, obtained by acyclical parthenogenesis under specified breeding conditions.
- Source: In-house laboratory culture with a known history.
- Age at study initiation:< 24 hours
- Method of breeding: With newborn daphnids, i.e. less than 3 days old, by placing about 250 of them into 10 litres of medium in an all-glass culture vessel.
- Feeding during test: No
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 180 mg/l expressed as CaCO3
- Test temperature:
- between 19.4 and 20.3°C
- pH:
- between 5.1 and 8.0
- Dissolved oxygen:
- between 1.7 and 9.5 mg/l
It should however be noted that especially at the highest test concentration it was observed that oxygen was very low at the study start (1.7 mg/l). The oxygen concentration at the end of the test had increased to a normal value of 8.1 mg/l. - Nominal and measured concentrations:
- At the start of the test, the mean measured concentrations were 0.19, 0.31, 0.86, 2.5 and 14.0 mg/l at the nominal concentrations of 10, 18, 32, 56 and 100 mg/l, respectively. The measured concentration after 48 hours decreased to 4.8 mg/l at nominally 100 mg/l. Measured concentrations in samples taken from nominally 10 to 56 mg/l could not be quantified (<0.1 mg/l). It was therefore not possible to determine average exposure concentrations for the range tested. Consequently, results were based on both nominal and initial measured exposure concentrations.
- Details on test conditions:
- - Type (delete if not applicable): open
- Material, size, headspace, fill volume: glass, 100 ml, normal headspace, 80 ml
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Adjusted ISO medium: the following chemicals (analytical grade) are dissolved in tap water purified by Reverse Osmosis (RO-water, GEON Waterbehandeling, Berkel-Enschot, The Netherlands):
Macro salts:
CaCl2.2H2O : 211.5 mg/l
MgSO4.7H2O: 88.8 mg/l
NaHCO3 : 46.7 mg/l
KCl : 4.2 mg/l
The hardness: 180 mg/l expressed as CaCO3 and the pH: 7.7 ± 0.3 after aeration.
- Culture medium different from test medium: yes, for culturing M7 medium is used
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hours photoperiod daily
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : EC50 t=24, 48 h and NOEC
TEST CONCENTRATIONS
- Spacing factor for test concentrations:
range finding: 10
main study: 1.8
- Test concentrations:control and 10, 18, 32, 56 and 100 mg/l - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate (K2Cr2O7,)
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 28 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% CI 24 - 32 mg/l.
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.66 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% CI:0.52-0.85 mg/l
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 28 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95 % CI:26 - 32 mg/l
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 18 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.31 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- Behavioural abnormalities: no
- Immobility of control: no immobility was observed.
- Other adverse effects control: no - Results with reference substance (positive control):
- The 24h-EC50 was 0.75 mg/l with a 95% confidence interval between 0.56 and 1.0 mg/l.
The 48h-EC50 was 0.36 mg/l with a 95% confidence interval between 0.28 and 0.49 mg/l. - Reported statistics and error estimates:
- Calculation of EC50:
The EC50-value was calculated at 24 and 48 hours of exposure from the probits of the percentages of affected daphnids and the logarithms of the corresponding test substance concentrations using the maximum likelihood estimation method (Finney, D.J., 1971: Probit analysis, Cambridge University Press, Cambridge, U.K., 3rd edition).
Any other information on results incl. tables
-
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of the present study Chloracetessigester did not induce significant acute immobilisation (<10%) of Daphnia magna at a nominal concentration of 18 mg/l after 48 hours of exposure (NOEC). This corresponded to an initial measured concentration of 0.31 mg/l.
The 48h-EC50 was 28 mg/l based on nominal concentrations (95% confidence interval between 24 and 32 mg/l) and was already reached after 24 hours of exposure. Based on initial measured concentrations this corresponded to 0.66 mg/l with a 95% confidence interval between 0.52 and 0.85 mg/l. - Executive summary:
-
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