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EC number: 253-256-2 | CAS number: 36888-99-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes (incl. QA statement)
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- - Test organism: Daphnia magna STRAUS (clone 5)
- Origin: Institut für Wasser-, Boden- und Lufthygiene (WaBoLu)
- Feeding: at least 5 times per week ad libitum with a mix of uicellular green algae
- Culture medium: Elendt M4 - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- pH:
- 7.49 - 7.73
- Dissolved oxygen:
- 6.78 - 8.23
- Nominal and measured concentrations:
- - Limit test concentration: 2 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessels: glass beakers (50 ml volume nominal)
- Fill volume: 20 ml
- Dilution water: prepared according to Directive 92/69/EEC Method C.2, Annex 1
- No. of organisms per vessel: 5
- No. of vessels per concentration and control: 4
- No. of study organisms: 20
FURTHER CONDITIONS
- Illumination: diffuse light, illumination strength max. 20 µE*m-2*s-1
- Photoperiod: 16/h light/dark cycle
- Feeding: Daphnids were not fed during study
- Age of Daphnids: 2 - 24 h old
- Cluture medium: Elendt M4 - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Results with reference substance (positive control):
- 24 h EC50 1.5 mg/L nominal test mat. mobility
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: the test solutions were prepared according to OECD guideline 23 by mixing 100.1 mg of test substance with 1 l test medium (M4). The mixture was placed in an ultrasonic bath for 5 minutes then stirred on a magnetic stir plate for about 3 days at 20 ± 2 °C to generate a
saturated test solution. Undissolved test substance was removed by filtration with a membrane filter (pore width 0.2 μm). - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Source: originally obtained from the Institute National de Recherche Chimique Appliquee, France and cultured in the laboratories of BASF since 1978.
- Age at study initiation (mean and range, SD): < 24 h - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Hardness:
- 2.20 – 3.20 mmol/l
- Test temperature:
- 19.3 – 20.0 °C
- pH:
- 7.8 - 8.1
- Dissolved oxygen:
- 8.1 - 8.9 mg/l
- Nominal and measured concentrations:
- Nominal test concentrations:
100 mg/l - Details on test conditions:
- TEST SYSTEM
- Test vessel: flat bottom glass tubes ( 20 ml)
- Fill volume: 10 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: A synthetic freshwater was used (Elendt M4).
- Ca/mg ratio: 4:1
- Conductivity: 550 - 650 μS/cm
OTHER TEST CONDITIONS
- Photoperiod: 16:8 h day-night regime
- Light intensity: About 20 – 850 lux at a wave length of 400 -750 nm;
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mobility after 0, 24 and 48 hours - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
Referenceopen allclose all
Description of key information
With high probability acutely not harmful to aquatic invertebrates.
Key value for chemical safety assessment
Additional information
One guideline study on the toxicity of the substance to Daphnia magna is available. In a static test system set up according to OECD guideline 202 an EC50 > 100 mg/l was determined after 48 hours (BASF SE, 2008).
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