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EC number: 205-205-0 | CAS number: 135-61-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2009-11-08 to 2009-11-12
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: study performed according to current guideline and GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
- Report date:
- 2010
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- 3-hydroxy-2'-methyl-2-naphthanilide
- EC Number:
- 205-205-0
- EC Name:
- 3-hydroxy-2'-methyl-2-naphthanilide
- Cas Number:
- 135-61-5
- Molecular formula:
- C18H15NO2
- IUPAC Name:
- 3-hydroxy-N-(2-methylphenyl)naphthalene-2-carboxamide
- Reference substance name:
- Naphtol AS-D
- IUPAC Name:
- Naphtol AS-D
- Details on test material:
- - Name of test material (as cited in study report): Naphtol AS-D
- Molecular formula: C18H15NO2
- Molecular weight: 277.33 g/mol
- Physical state: Solid, beige powder
Constituent 1
Constituent 2
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: The saturated solution and the control were analytically verified via HPLC in the fresh media (0 hours) and in the old media (48 hours).
- Sampling method: For the old media, separate replicates without test organisms were prepared and incubated under test conditions. The recovery rates of the test item should be ± 20 % of the initially measured. Otherwise the effect levels have to be given based on the geometric mean measured concentration.
- Sample storage conditions before analysis: All samples were stored at 6 ± 2 °C until start of analysis, if necessary.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution (100 mg/L) was prepared with dilution water two days prior to application.
- Dispersion treatment: The stock solution was shaken with 20 rpm for 48 hours (rotating shaker 3040, GFL). Undissolved particles were removed by membrane filtration (membrane filter 0.45 µm, RC, MACHEREY-NAGEL).
- Eluate: Dilution water
- Differential loading: Limit concentration: Saturated solution, corresponding to a geometric mean measured test item concentration of 0.108 mg/L.
- Controls: Dilution water without test item tested under the same conditions as the test groups.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna (STRAUS)
- Strain: Clone 5
- Source: Institut für Wasser-, Boden- Lufthygiene (WaBoLu), further own breeding
- Age at study initiation (mean and range, SD): 2 to 24 h
- Method of breeding: In 2-3 L glass vessels with appr. 1.8 L culture medium, at 20 +/- 2 °C, in an incubator, 16 h illumination, illumination strength max. 20 µExm-2 x s-1
- Feeding during test: No feeding
ACCLIMATION
- Acclimation period: 2 h
- Acclimation conditions (same as test or not): Same as test
- Type and amount of food: No feeding
- Health during acclimation (any mortality observed): Healthy, no mortality observed
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Post exposure observation period:
- No
Test conditions
- Hardness:
- Dilution water at test start: 263 (mg CaCO3/L)
- Test temperature:
- 20.5 °C
- pH:
- Water Quality Parameters at Test Start (0 hours, New Media)
(measured in one additional replicate of the limit concentration and control)
pH-Value
Saturated solution 7.42
Control 7.98
Water Quality Parameters at Test End (48 hours, Old Media)
(measured in all replicates)
pH-Values
Replicates
1 2 3 4
Saturated solution 7.56 7.57 7.57 7.56
Control 7.47 7.51 7.51 7.53 - Dissolved oxygen:
- Water Quality Parameters at Test Start (0 hours, New Media)
(measured in one additional replicate of the limit concentration and control)
Dissolved O2 - Concentration
[mg/L]
Saturated solution 7.67
Control 8.37
Water Quality Parameters at Test End (48 hours, Old Media)
(measured in all replicates)
Dissolved O2 - Concentration [mg/L]
Replicates
1 2 3 4
Saturated solution 7.18 7.48 7.44 7.35
Control 7.32 7.27 7.49 7.47 - Salinity:
- Freshwater
- Nominal and measured concentrations:
- Please refer to "Any other information on materials and methods"
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass beakers, 50 mL
- Type (delete if not applicable): closed with parafilm
- Material, size, headspace, fill volume: Glass beakers (4 cm ID x 7 cm H), 50 mL
- Aeration: No
- Renewal rate of test solution (frequency/flow rate): One application at test start, static test
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): None
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Acc. to Directive 92/69/EEC Method C.2, Annex 1
- Conductivity: Dilution water at test start: 640 µS/cm
- Culture medium different from test medium: Elendt M4 acc. to ELENDT (1990), modified to a total hardness of 160 to 180 mg CaCO3/L
- Intervals of water quality measurement: At test start and test end
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16:8 light:dark cycle
- Light intensity: Diffuse light, illumination strength max. 20 µE x m-2 x s-1
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
TEST CONCENTRATIONS
- Spacing factor for test concentrations: None, limit test
- Range finding study
Immobilization Rates of the Preliminary Range Finding Test [%]
(n = 20, divided into 2 replicates with 10 daphnids each)
Nominal Loading
of the
Saturated Solution
[mg/L] IMMOBILIZATION [%]
24 hours 48 hours
Replicates Replicates
1 2 MV 1 2 MV
100 0 0 0 0 10 5
Control 0 0 0 0 0 0
- Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrations
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 0.108 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Behavioural abnormalities: None
- Mortality of control: 0%
- Other adverse effects control: None
- Abnormal responses: None
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: None - Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- EC50/LC50: 1.67 (CI 1.63 - 1.71) mg/L - Reported statistics and error estimates:
- The effects of the saturated solution were evaluated directly from the observation data.
Any other information on results incl. tables
Percentage of Daphnids Incapable of Swimming after 24 and 48 h of Exposure
(n = 20, divided into 4 replicates with 5 daphnids each)
IMMOBILIZATION [%] |
||||||||||
Naphtol AS-D Geometric Mean Measured Concentration [mg/L] |
24 hours |
48 hours |
||||||||
Replicates |
Replicates |
|||||||||
1. |
2. |
3. |
4. |
MV |
1. |
2. |
3. |
4. |
MV |
|
0.108 (Saturated solution) |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
Control |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- At the saturated solution of the test item Naphtol AS-D with a nominal loading of 100 mg/L, corresponding to a geometric mean measured concentration of 0.108 mg/L, no effect to Daphnia magna was observed.
- Executive summary:
In the acute immobilization test with Daphnia magna (STRAUS) the effects of the saturated solution of the test item Naphtol AS-D with a nominal loading of 100 mg/L as limit concentration were determined according to OECD 202 (2004). The limit test was conducted under static conditions over a period of 48 hours.
EC-Values, NOEC and LOEC
(based on the geometric mean measured test item concentration)
Test Duration
[h]Geometric Mean Measured
Test Item Concentration
[mg/L]
Confidence Interval
p = 95%
[mg/L]EC50
24
> 0.108
not applicable
48
> 0.108
not applicable
EC100
24
> 0.108
---
48
> 0.108
---
NOEC
48
0.108
---
LOEC
48
> 0.108
---
At the saturated solution of the test item Naphtol AS-D
with a nominal loading of 100 mg/L,
corresponding to a geometric mean measured concentration of 0.108 mg/L,
no effect to Daphnia magna was observed.
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