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EC number: 203-013-1 | CAS number: 102-20-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Remarks:
- Experimental data of read across substances
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Justification for type of information:
- Data for the target chemical is summarized based on the read across chemicals
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- no guideline available
- Principles of method if other than guideline:
- WoE report is based on two long term toxicity to aquatic invertebrates studies as-
WoE 2 and WoE 3. - GLP compliance:
- not specified
- Analytical monitoring:
- yes
- Details on sampling:
- WoE 2- Concentrations: (nominal values) control, vehicle control, - 0.040, 0.097, 0.240, 0.580, 1.40 mg/L ,geometric ratio: 2.4
- Vehicle:
- yes
- Remarks:
- WoE 2- Dimethylformamide
- Details on test solutions:
- WoE 2- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Dimethylformamide
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)): constant concentration 100 microL/L - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- WoE 2, WoE 3: Common Name: Water flea
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
- Test temperature:
- WoE 2- 20 ±1 °C
- Nominal and measured concentrations:
- WoE 2- (nominal values) control, vehicle control, - 0.040, 0.097, 0.240, 0.580, 1.40 mg/L ,geometric ratio: 2.4 But 1.40 mg/L was the adjustable highest concentration of test solution.
- Details on test conditions:
- WoE 2- TEST SYSTEM
- Type (delete if not applicable): closed, covered water surface with teflon sheet
- Material, size, headspace, fill volume: test solution volume 80 mL/vessel
- Renewal rate of test solution (frequency/flow rate): total amount of test solution every day
- No. of organisms per vessel: 10/concentration (1/vessel)
- No. of vessels per concentration (replicates): 10
OTHER TEST CONDITIONS
- Photoperiod: 16 hours light/8 hours dark
- Light intensity: less than 800 lux
TEST CONCENTRATIONS
- Spacing factor for test concentrations: geometric ratio 2.4
- Test concentrations: 0.040, 0.097, 0.240, 0.580, 1.40 mg/L - Reference substance (positive control):
- not specified
- Key result
- Duration:
- 21 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 1.18 - < 1.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Remarks on result:
- other: EC50 value of test chemical was determined to be > 1.18 to < 1.2 mg/l.
- Key result
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 1.18 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Remarks on result:
- other: LOEC value of test chemical was determined to be 1.18 mg/l
- Key result
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- > 0.17 - < 0.519 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Remarks on result:
- other: NOEC value of test chemical was determined to be > 0.17 to < 0.519 mg/l.
- Validity criteria fulfilled:
- not specified
- Conclusions:
- On the basis of the experimental studies of the structurally similar read across chemical and applying the weight of evidence approach and by evaluating the effect of test chemical on test organism, daphnia magna the 21 days NOEC, LOEC and EC50 value was determined to be > 0.17 to < 0.519 mg/l, 1.18 mg/l and > 1.18 to < 1.2 mg/l respectively.
- Executive summary:
Data available of the structurally similar read across chemicals has been reviewed to determine the effect of the test chemical on aquatic invertebrates. The studies are as mentioned below:
The first study of long term toxicity to aquatic invertebrates was carried out according to OECD 211 guideline. The test organisms was taken as daphnia magna. The test was performed in semi-static conditions. The duration of study was 21 days. Dimethylformamide was used as a vehicle with a concentration of 100 microL/L. Test concentrations were taken as (nominal values) control, vehicle control of 0.040, 0.097, 0.240, 0.580, 1.40 mg/L in the geometric ratio: 2.4. The adjustable highest concentration of test solution was 1.40 mg/l. Test vessel was of closed, covered water surface with teflon sheet. Test solution volume was taken as 80 mL/vessel. Total amount of test solution was renewed every day. Number of organisms per vessel was taken as 10 per concentration, one test organism per vessel was taken for study. The number of vessels per concentration (replicates) was taken as ten. Photoperiod was given for 16 hours light with 8 hours darkness. Light intensity provided was less than 800 lux. Test conditions includes the temperature of 20 ±1 °C. Analytical monitoring was done by HPLC. On the basis of reproduction inhibition effect of test chemical on daphnia magna the 21 days NOEC, LOEC and EC50 value of test chemical was determined to be 0.519 mg/l, 1.18 mg/l and > 1.18 mg/L respectively. On the basis of the value of NOEC the test chemical was classified as 'Chronic category 2' as per CLP criteria.
The second study of long-term toxicity to aquatic invertebrates was carried out according to OECD 211 guideline. The test organisms was taken as daphnia magna. The test was performed in semi-static conditions. the duration of study was 21 days. On the basis of reproduction inhibition effect of test chemical on daphnia magna the 21 days EC50 and NOEC value of test chemical was determined to be 1.2 mg/l and 0.71 mg/l respectively. On the basis of the value of NOEC the test chemical was classified as Chronic category 2 as per CLP criteria.
On the basis of the experimental studies of the structurally similar read across chemical and applying the weight of evidence approach and by evaluating the effect of test chemical on test organism, daphnia magna the 21 days NOEC, LOEC and EC50 value was determined to be > 0.17 to < 0.519 mg/l, 1.18 mg/l and > 1.18 to < 1.2 mg/l respectively. Based on the value of NOEC the test chemical was classified as 'Chronic category 2' as per CLP criteria.
Reference
Description of key information
On the basis of the experimental studies of the structurally similar read across chemical and applying the weight of evidence approach and by evaluating the effect of test chemical on test organism, daphnia magna the 21 days NOEC, LOEC and EC50 value was determined to be > 0.17 to < 0.519 mg/l, 1.18 mg/l and > 1.18 to < 1.2 mg/l respectively.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- NOEC
- Effect concentration:
- > 0.17 - < 0.519 mg/L
Additional information
Data available of the structurally similar read across chemicals has been reviewed to determine the effect of the test chemical on aquatic invertebrates. The studies are as mentioned below:
The first study of long term toxicity to aquatic invertebrates was carried out according to OECD 211 guideline. The test organisms was taken as daphnia magna. The test was performed in semi-static conditions. The duration of study was 21 days. Dimethylformamide was used as a vehicle with a concentration of 100 microL/L. Test concentrations were taken as (nominal values) control, vehicle control of 0.040, 0.097, 0.240, 0.580, 1.40 mg/L in the geometric ratio: 2.4. The adjustable highest concentration of test solution was 1.40 mg/l. Test vessel was of closed, covered water surface with teflon sheet. Test solution volume was taken as 80 mL/vessel. Total amount of test solution was renewed every day. Number of organisms per vessel was taken as 10 per concentration, one test organism per vessel was taken for study. The number of vessels per concentration (replicates) was taken as ten. Photoperiod was given for 16 hours light with 8 hours darkness. Light intensity provided was less than 800 lux. Test conditions includes the temperature of 20 ±1 °C. Analytical monitoring was done by HPLC. On the basis of reproduction inhibition effect of test chemical on daphnia magna the 21 days NOEC, LOEC and EC50 value of test chemical was determined to be 0.519 mg/l, 1.18 mg/l and > 1.18 mg/L respectively. On the basis of the value of NOEC the test chemical was classified as 'Chronic category 2' as per CLP criteria.
The second study of long-term toxicity to aquatic invertebrates was carried out according to OECD 211 guideline. The test organisms was taken as daphnia magna. The test was performed in semi-static conditions. the duration of study was 21 days. On the basis of reproduction inhibition effect of test chemical on daphnia magna the 21 days EC50 and NOEC value of test chemical was determined to be 1.2 mg/l and 0.71 mg/l respectively. On the basis of the value of NOEC the test chemical was classified as Chronic category 2 as per CLP criteria.
On the basis of the experimental studies of the structurally similar read across chemical and applying the weight of evidence approach and by evaluating the effect of test chemical on test organism, daphnia magna the 21 days NOEC, LOEC and EC50 value was determined to be > 0.17 to < 0.519 mg/l, 1.18 mg/l and > 1.18 to < 1.2 mg/l respectively. Based on the value of NOEC the test chemical was classified as 'Chronic category 2' as per CLP criteria.
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