Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 221-374-3 | CAS number: 3081-01-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Long-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2010-04-29 till 2010-06-28
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP study, well described, performed according to a current OECD guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.20 (Daphnia magna Reproduction Test)
- Deviations:
- no
- Principles of method if other than guideline:
- The method described in the Council Regulation (EC) No 440/2008, Method C.20 'Daphnia magna Reproduction Test'' (2008), which equals to the OECD Test guideline 211 (Daphnia magna Reproduction Test; adopted: Sept. 21, 1998), assesses adverse effects of various concentrations of a test item to a freshwater micro crustacean species within a 21 day exposure period under semi-static conditions.
The purpose of this method was to determine the NOEC and the LOEC for the parthenogenetic reproduction rate and, if possible, for the mortality/ immobilisation rate of parent Daphnia. If no adverse effects were recorded at a maximum test concentration of 10 mg/L (LOEC > 10 mg/L) no higher concentrations need to be tested. - GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - examined by chemical analysis (HPLC) at 0 and 21 days
- Vehicle:
- no
- Details on test solutions:
- A stock solution was prepared to give the desired series of test concentrations. To achieve this 4.9 – 5.3 mg of the test item were added to 1 litre of dilution water (one exception on 2010-04-29 was made, 10.3 mg were added to 2 litres dilution water), treated for 1 h in an ultrasonic bath and stirred for 24 h on a magnetic stirrer. Finally undissolved particles of the test item were removed by filtration using folded filters of pore size 7-12 µm. The pH was measured to be pH 7.8 – 8.0.
To produce the different test item concentrations appropriate amounts of the stock solution were diluted with dilution water to a volume of 100 mL per replicate. For each test item concentration and control 10 replicates were prepared. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Test species
- Name: Daphnia magna STRAUS, parthenogenetic females
- Source: Strain of Bundesgesundheitsamt Berlin
- Maintenance and Acclimatisation: A population of parthenogenetic females of synchronized age structure has been maintained for more than 15 years in the test facility under constant temperature conditions (20 +/- 1 °C) at a 16 : 8 hour light-dark photoperiod (illumination: < 1000 lux). The culture water (so-called 'M4 medium') was partly renewed once a week. The Daphnia were exclusively fed with unicellular green algae (Desmodesmus subspicatus) 'ad libitum'. Mortalities of parent Daphnia during the culture period were recorded daily in a semi-quantitative way. The neonates were separated from their parent Daphnia by filtration prior to the acute test. - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
- Hardness:
- 14.7 – 15.5 °dH (= 262.4 – 276.7 mg/L CaCO3)
- Test temperature:
- temperature range: 18 - 22 °C with a maximum temperature fluctuation of +/- 1 °C
- pH:
- 8.0 - 8.3
- Dissolved oxygen:
- 8.5 - 9.6 mg/L
- Nominal and measured concentrations:
- The following nominal concentrations of the test item were tested in the acute test: 0.21, 0.47, 1, 2.3 and 5 mg/L.
- Details on test conditions:
- Exposure conditions
- Test vessels: 150 mL glass beakers holding 1 neonate in 100 mL of test medium
- Experimental design: 5 test concentration plus 1 control, 1 neonate per vessel, 10 replicates per concentration/control, semi-static system (renewal of the test media on Monday, Wednesday and Friday) respectively.
- Method of initiation: neonates were placed in prepared media
- Photoperiod: 16 h light: 8 h dark
- Light intensity: not exceeding 800 lux
- Temperature of incubation unit: 19.0 to 21.0 °C
- Aeration: none
- Test item concentration/s: 0.0032, 0.01, 0.032, 0.1 and 0.32 mg/L
- Food source and : Desmodesmus subspicatus
- feeding rate:day 0-7: 0.1 mg C / Daphnia / day
day 8-21: 0.2 mg C / Daphnia / day
- Duration of exposure: 21 days
- Criteria of effects: The criterion of adverse effects used in this study was the item-induced inhibitory effect on the parthenogenetic reproduction rate and the mobility behaviour/mortality rate of parent Daphnia, all effects being assessed at least three times a week - Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.028 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 0.087 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Details on results:
- The results are expressed in terms of time-weighted mean measured concentrations. Effective concentrations ranged from 95.0 – 103.1 % of the nominal values in the freshly prepared media and from 68.8 – 92.0 % in the media after 48 hours, or 72 hours of exposure, respectively.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The following validity criteria of the test were met:
The mortality rate in the controls did not exceed 20 % by the end of the test.
Living offspring produced per parent Daphnia surviving at the end of the test was = 60 in the controls.
The dissolved oxygen concentration remained above 3 mg/L throughout the exposure period.
The pH was within the range 6-9.
Water hardness was above the recommended value of 140 mg/L (as CaCO3). - Executive summary:
The chronic toxicity of 4 -Hydroxydiphenylamine to Daphnia magna was investigated in a static test according to Council Regulation (EC) No 440/2008, Method C.20 'Daphnia magna Reproduction Test'' (2008), which equals to the OECD Test guideline 211 (Daphnia magna Reproduction Test; 1998). After 21 days a NOEC of 0.028 mg/L and a LOEC of 0.087 mg/L was determined (Currenta, 2010).
Reference
Description of key information
The chronic toxicity of 4 -Hydroxydiphenylamine to Daphnia magna was investigated in a static test according to Council Regulation (EC) No 440/2008, Method C.20 'Daphnia magna Reproduction Test'' (2008), which equals to the OECD Test guideline 211 (Daphnia magna Reproduction Test; 1998). After 21 days a NOEC of 0.028 mg/L was determined.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 0.028 mg/L
Additional information
4-Hydroxydiphenylamine (4 -HPDA) is the hydrolysis product from N-(1,4-dimethylpentyl)-N'-phenylbenzene-1,4-diamine (7PPD). A secondary hydrolysis product is p-hydroquinone.
The lowest effect value in long-term tests was found for p-hydroquinone (0.007 mg/L, expressed in 7PPD equivalents) which is lower than the value for 4-HDPA (0.028 mg/L).Although p-hydroquinone is a secondary hydrolysis product of 7PPD, it was not chosen as a source of key value as the formation of p-Hydroquinone from 7PPD by hydrolysis is rather slow.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
![ECHA](/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/echa_logo.png)