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EC number: 425-020-0 | CAS number: 191680-81-6 CGL 116
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to soil macroorganisms except arthropods
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to soil macroorganisms except arthropods: short-term
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2013
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- yes
- Details on preparation and application of test substrate:
- Based on the specified water solubility the application of the test substance via an aqueous stock solution is not possible. The water solubility is not enough to prepare a sufficient stock solution with deionized water. Application of the test substance: ground in a mortar and distributed in quartz sand and added to the test substrate afterwards. The required amounts of test substance were weighed into a beaker and mixed with 25 g quartz sand.
Preparing the control assays:
For preparation of the test assays of the control, the amount of about 2535 g dry test was added to a bowl. The untreated quartz sand aliquot was added after and thoroughly mixed with the test substrate. Than the required volume of 715 mL deionized water was added to the test substrate, respectively and mixed well again. Four portions of 750 g of wet substrate were distributed to preserving jars.
Preparing the test substance assays:
For preparation of the test assays with test substance amounts of about 2535 g dry test substrate was added to a box. The quartz sand aliquots treated with the required test substance amounts were added and thoroughly mixed with the test substrate. Than the required volumes of 715 mL deionized water was added to the test substrate test substance mixtures and mixed well again. Four portions of 750 g of the treated wet test substrate for each test concentration were distributed to preserving jars. - Test organisms (species):
- Eisenia fetida
- Animal group:
- annelids
- Details on test organisms:
- Species: Earthworm Eisenia Fetida
Origin: ECT, Ecotoxicology GmbH, Böttgerstr. 2-14, D-65349 Flörsheim, Germany
Feeding of the earthworms: The worms were feed with oat flakes at least every second week.
Age of the animals at the start of exposure: approx. 6 months (guaranteed by the supplier)
Test animals: Animals with a weight of 325 mg – 421 mg (calculated values from the mean values of replicates) and a clear visible clitellum were used for the test
Conditions of storage at the test facility: The worms were stored in the transport box until adaptation at room temperature in the dark.
Adaptation: Stored in test substrate at test conditions over night before start of exposure
Reason for selection of species: Recommended species in the Test guidelines, its susceptibility to chemicals resembles that of true soil inhabiting species, it has a short life cycle, hatches from cocoons in 3 to 4 weeks and reaches maturity in seven to eight weeks at 20 °C - Study type:
- laboratory study
- Substrate type:
- artificial soil
- Limit test:
- no
- Total exposure duration:
- 14 d
- Test temperature:
- 20 ± 2°C
- pH:
- pH value of the dry test substrate: 6.3 mean value of 2 single values (6.3 and 6.3, rounded to 2 significant digits)
- Moisture:
- Calculated water content of the test substrate at start of exposure: 30 % of dry weight
Water content of the test substrate at the end of the exposure period: 29.9 % of dry weight (single measurement) - Details on test conditions:
- TEST SYSTEM
- Test container: Preserving jars, volume 1 L, covered with glass lids
- Amount of soil or substrate: 750 g ± 1 g artificial soil (wet weight)
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 4 test vessels per concentration
- No. of replicates per control: 4
OTHER TEST CONDITIONS
- Photoperiod: Continuously, over the exposure period
- Light intensity: 546 Lux, mean value of 5 single values (717, 514, 483, 521, 494 [Lux]) at the start of exposure
EFFECT PARAMETERS MEASURED: Mortality
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2 - Nominal and measured concentrations:
- Nominal concentration: 0 (control), 62.5, 125, 250, 500, 1000 mg/kg soil dry weight
- Reference substance (positive control):
- yes
- Remarks:
- 2-Chloroacetamide
- Key result
- Duration:
- 14 d
- Dose descriptor:
- LC50
- Effect conc.:
- > 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 14 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- - Mortality at end of exposure period: none
- Any other observations: No abnormal behaviour or toxic symptoms of the worms were observed in the test substance groups or control group during the exposure. - Results with reference substance (positive control):
- - Results with reference substance: The LC50 value of the reference substance for the earthworm Eisenia fetida after 14 days was not in the range of 20 mg/kg to 80 mg/kg with a value of 12.6 mg/kg. Although the LC50 is outside of the recommended range of 20-80 mg/kg the test system showed that it was sufficiently sensitive and was suitable to determine the acute toxicity of the test substance.
- Validity criteria fulfilled:
- yes
Reference
Description of key information
No toxic effects to Eisenia fetida could be observed at a concentration of 1000 mg/kg dw.
Key value for chemical safety assessment
- Short-term EC50 or LC50 for soil macroorganisms:
- 1 000 mg/kg soil dw
Additional information
The toxicity of the test substance to terrestrial organisms was investigated in a GLP guideline study according to OECD 207. In this study, no toxic effects to Eisenia fetida were observable after 14 days at the highest concentration test of 1000 mg/kg dw (BASF SE 67E0252/12G286, 2013).
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