Sulfuric acid, mono-C8-18-alkyl esters, magnesium salts, compds. with triethanolamine

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Batch no.: 0016420429
- Purity: ca. 76 %
- Impurities: 17.9 % triethanolamine, 2.5 % sulfates, 2.5% Mg, 1.6% water, 0.05 % cyclohexanol


STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
The stability of the test substance under storage conditions over
the test period was guaranteed by the sponsor, and the sponsor
holds this responsibility.

Analytical monitoring:

yes

Details on sampling:

Samples for concentration control analysis were taken from freshly prepared solution (0 h and 24 h) in additionally prepared vessels without daphnids and at the end of each exposure interval (after approx. 24 h and 48 h) from all test vessels with mobile daphnids (pooled replicates) or from all remaining test concentrations.
Additionally, samples were collected from the 24-hour old abiotic replicate (no Daphnia) per test group.

Vehicle:

no

Details on test solutions:

Each test solution was prepared separately (differential loading) by directly adding test
substance to test medium. For each concentration, the test substance was weighed into a
volumetric flask and filled to the mark with test medium. The solution was then stirred for approximately 1 hour. The solutions were checked visually to ensure that the test substance is completely dissolved. All prepared solutions were visibly clear. The control solutions were
treated in the same way.
Fresh test solutions were prepared and renewed daily

Test organisms (species):

Daphnia magna

Details on test organisms:

- Source: The clone of Daphnia magna STRAUS used was supplied by Institut National de Recherche Chimique AppIiquée, France, in 1978. The daphnids are cultured under standard conditions in the laboratory.
- Age at study initiation: < 24 hours
- Age of stock animals: 2 - 4 weeks
- Culture water: Synthetic M4 medium
- Feeding during test: no

Test type:

semi-static

Water media type:

freshwater

Remarks:

Synthetic M4 medium

Limit test:

no

Total exposure duration:

48 h

Hardness:

2.2 - 3.2 mmol/L

Test temperature:

19.8 - 20.2 °C

pH:

7.5 - 8-5

Dissolved oxygen:

8.8 - 9.3 mg/L

Conductivity:

550 - 650 µS/cm

Nominal and measured concentrations:

Nominal: 0, 0.22, 0.46, 1.0, 2.2, 4.6, 10 mg/L
Mean measured: 0, 0.188, 0.393, 0.855, 1.790, 3.880, 9.070 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass beakers (nominal volume 100 mL)
- Type : open
- Volume of solution: 50 mL
- Aeration: none
- Renewal rate of test solution (frequency): 24h
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 0.1 animals/mL

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Elendt M4
- Total organic carbon: < 2 mg/L
- pH: 7.5-8.5
- Ca/mg ratio: 4:1
- Conductivity: 550-650 µS/cm



OTHER TEST CONDITIONS
- Adjustment of pH: 8.0
- Photoperiod: day/night 16:8 hours
- Light intensity: 132-794 lux at a wave length of 400-750 nm

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Immobilisation after 24h and 48h

Reference substance (positive control):

yes

Remarks:

sodium chloride

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

3.84 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

10.011 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Results with reference substance (positive control):

EC50: 4.63 g/L

Validity criteria fulfilled:

yes

Conclusions:

The substance is acutely toxic to aquatic invertebrates.

Description of key information

EC50 (48h) = 3.84 mg/L (mean measured) for immobilisation of Daphnia magna (OECD 202)

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

EC50

Effect concentration:

3.84 mg/L

Additional information

One study investigating the short-term toxicity of the test substance to aquatic invertebrates is available. The study was conducted according to OECD 202 and GLP using Daphnia magna as test organism (BASF SE, 2020). Nominal concentrations between 0.22 and 10 mg/L were tested. The exposure was analytically verified. No immobilized daphnids were observed in the control. After an exposure time of 48h the EC50 is determined to be 3.84 mg/L basd on mean measured concentrations. Thus, the test substance can be considered as acutely toxic to aquatic invertebrates.