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EC number: 801-829-8 | CAS number: 1247790-47-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- from 2012-09-28 to 2012-11-05
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study without deviations and test procedure according to GLP standards
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 012
- Report date:
- 2012
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 3,6-dimethylheptan-2-ol
- EC Number:
- 801-829-8
- Cas Number:
- 1247790-47-1
- Molecular formula:
- C9H20O
- IUPAC Name:
- 3,6-dimethylheptan-2-ol
Constituent 1
Method
- Target gene:
- his (Salmonella), trp (E. coli)
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 mix
- Test concentrations with justification for top dose:
- Doses selected for the preliminary test: 1.2; 4.9; 20; 78; 313; 1250; and 5000 µg/plate
Doses selected for all strains both with and without metabolic activation in the main test. : 10; 20; 39; 78; 156; 313 µg/plate - Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO
- Justification for choice of solvent/vehicle: from the result of the solubility test, the test substance was insoluble at 50 mg/mL in water, and was dissolved at 50 mg/mL in DMSO and at 100 mg/mL in acetone. And neither exothermic reaction nor generation of gas was observed.
Controlsopen allclose all
- Untreated negative controls:
- yes
- Remarks:
- treated with only the DMSO used to prepare the test solution
- Positive controls:
- yes
- Positive control substance:
- other: 2-(2-furyl)-3-(5-nitro-2-furyl)acrylamide
- Remarks:
- Positive control for: strain TA100 without metabolic activation at 0.01 µg/plate, strain WP2 uvrA without metabolic activation at 0.01 µg/plate and strain TA98 without metabolic activation at 0.1 µg/plate.
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- Remarks:
- Positive control for strain TA1535 without metabolic activation at 0.5 µg/plate
- Positive controls:
- yes
- Positive control substance:
- other: 2-methoxy-6-chloro-9-(3-(2-chloroethyl)aminopropylamino)acridine.2HCl (ICR-191)
- Remarks:
- Positive control for strain TA1537 without metabolic activation at 1.0 µg/plate
- Positive controls:
- yes
- Positive control substance:
- other: 2-Aminoanthracene
- Remarks:
- Positive control for strain TA1535 with metabolic activation at 2.0 µg/plate and for strain WP2 uvrA with metabolic activation at 10 µg/plate.
- Positive controls:
- yes
- Positive control substance:
- benzo(a)pyrene
- Remarks:
- Positive control for strain TA100 with metabolic activation at 5.0 µg/plate, for strain TA98 with metabolic activation at 5.0 µg/plate and for strain TA1537 at 5.0 µg/plate.
- Details on test system and experimental conditions:
- Pre-culture procedure:
A bacterial suspension of each strain (20 µL of S. typhimurium TA strains, 5 µL of E.coli WP2 uvrA) was inoculated into an L-form culture tube (35 mL capacity) containing 10 mL of Nutrient Broth. This culture tube was left at 4 °C until strating incubation, and then incubated while shacking (100 rpm) in a water bath at 37 °C for 8 hours. After incubation, the optical density was measured and the number of viable cells was calculated by growth curve for each strain. The bacterial cultures were stored at room temperature until strarting of the test.
Test procedures: preincubation method - Evaluation criteria:
- In the two main tests, if the number of revertant colonies on the test plates increased significantly in comparison with that on the control plates (based on teice as many as that of the negative control), and dose-response and reporoducibility were also observed, the test substance was judged to be positive. The results at each concentration were demonstrated with the mean and the standard deviation.
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and E. coli WP2
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- growth inhibition was observed at 313 µg/plate in all strains
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- In the two main tests, neither an increase of revertant colonies (more than twice as many as that of the negative control) nor a dose-related response was observed at any doses in any strains of base-pair substitution type or frame-shift type, with or without metabolic activation.
The revertant colonies of the positive control showed an increase of more than twice that of the negative controls and they were within limits of controls (mean ± 3SD) in historical data, indicating that this study was performed correctly.
From these results, mutagenicity of the test substance was judged negative.
The growth inhibition of the test strains by the test substance was observed was observed at the doses marked with "*" in the tables. And the precipitate of the test substance on the plates was not observed either with or without metabolic activation. - Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
The results are shown in tables 1 to3 and figures 1 and 2. The figures are based on table 3
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
There was no evidence of induced mutant colonies over background in any of the tester strains in the presence or absence of mammalian metabolic
activation in this study. - Executive summary:
Mutagenicity potential of 3,6 -dimethylheptan-2 -ol was assessed with Salmonella typhimurium TA100, TA1535, TA98, TA1537 and Eschrichia coli WP2 uvrA. In this study, neither an increase of revertant colonies more than twice in comparison with that of the negative control nor a dose-related response was observed in any strains of base-pair substitution type or frame-shift type, with or without metabolic activation.
From the above, it is judged 3,6 -dimethylheptan-2 -ol has no mutagenicity forward to bacteria under the described study conditions.
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