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EC number: 221-070-0 | CAS number: 2997-92-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 26.2.-15.3.2013
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Vehicle:
- no
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna Straus (water flea)
- Strain: Berlin
- Source: Umweltbundesamt Berlin
- Age at study initiation (mean and range, SD): less than 24 hours
- Weight at study initiation (mean and range, SD):
- Length at study initiation (length definition, mean, range and SD):
- Valve height at study initiation, for shell deposition study (mean and range, SD):
- Peripheral shell growth removed prior to test initiation:
- Method of breeding: similar to EN ISO 6341
- Feeding during test
- Food type: no feeding
- Amount: -
- Frequency: -
ACCLIMATION
- Acclimation period:
- Acclimation conditions (same as test or not):
- Type and amount of food: green algae (Desmodesmus subspicatus)
- Feeding frequency:
- Health during acclimation (any mortality observed):
QUARANTINE (wild caught)
- Duration:
- Health/mortality: - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 2.502 mmol/L
- Test temperature:
- 19.4 - 20.6 °C
- pH:
- 7.7 - 7.8
- Dissolved oxygen:
- see Table 2
- Nominal and measured concentrations:
- nominal: control, 1.0, 2.2, 4.6, 10, and 22 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 50 mL beaker
- Type (delete if not applicable): open / closed
- Material, size, headspace, fill volume: glass, 20 ± 5 mL test solution
- Aeration:
- Type of flow-through (e.g. peristaltic or proportional diluter):
- Renewal rate of test solution (frequency/flow rate): 24 ± 1 h
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): -
- Biomass loading rate:
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: M4 medium
- Total organic carbon:
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine:
- Alkalinity:
- Ca/mg ratio:
- Conductivity:
- Culture medium different from test medium:
- Intervals of water quality measurement:
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: none
- Light intensity: no
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : immobilisation
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2.2
- Justification for using less concentrations than requested by guideline:
- Range finding study
- Test concentrations:
- Results used to determine the conditions for the definitive study: - Reference substance (positive control):
- yes
- Remarks:
- K2Cr2O7
- Duration:
- 24 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 4.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 13 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 3.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Results with reference substance (positive control):
- - Results with reference substance valid? yes; EC50 within demanded range of 0.6-1.7 mg/L.
- Mortality:
- EC50/LC50: 1.5 mg/L
- Other: - Validity criteria fulfilled:
- yes
- Remarks:
- all validity criteria are fulfilled
- Conclusions:
- Based on the experimental observations an effect concentration EC50 (48h) 3.5 mg/L was calculated. As all validity criteria were met in this study the results are considered to be adequate and were used in the further assessment of 2,2'-azobis(2-amidinopropane)-dihydrochloride.
- Executive summary:
All validity criteria were met. For the estimation of the EC50s of the test item, the fits showed sufficient statistical correspondence of the data with the dose-response-equation.
Potassium dichromate K2Cr2O7 (CAS No. 7778 -50 -9) was used as positive control in a current reference study (201207R201). The value was determined as 1.5 mg/L, lying within the demanded range of 0.6 -1.7 mg/L. No significant immobilisation occured in the control.
The recovery after 24 hours ranged from 85 % to 89 % of the start concentration. After 48 hours, recovery ranged from 81 % to 100 %. The correlation between nominal and measured concentration was good with exception the lowest concentrated treatment containing 1.0 mg/L (lower than LOQ). Therefore, the determination of the biological results was based on the geometric mean of the measured concentrations.
No observations were made which might cause doubts concerning the validity of the study outcome. The resukt of the test is considered valid.
Reference
nominal concentration (mg/L) |
Immobility 24 hours |
Immobility 48 hours |
||||||||
abs. |
in % |
abs. |
in % |
|||||||
0 |
0 |
1 |
0 |
0 |
5 |
0 |
1 |
0 |
0 |
5 |
1.0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
2.2 |
0 |
0 |
0 |
0 |
0 |
5 |
0 |
0 |
1 |
30 |
4.6 |
0 |
0 |
1 |
0 |
5 |
1 |
5 |
5 |
2 |
65 |
10 |
1 |
3 |
1 |
1 |
30 |
5 |
5 |
5 |
5 |
100 |
22 |
5 |
5 |
3 |
4 |
85 |
5 |
5 |
5 |
5 |
100 |
Table 1. Immobilities
nominal concentration (mg/L) |
O2-Concentration in mg/L |
|||
0 h |
24 h old |
24 h new |
48 h |
|
0 |
8.6 |
8.5 |
8.5 |
9.1 |
1.0 |
8.6 |
8.6 |
8.5 |
8.6 |
2.2 |
8.6 |
8.6 |
8.6 |
8.6 |
4.6 |
8.3 |
8.5 |
8.7 |
8.2 |
10 |
8.4 |
8.6 |
8.7 |
8.1 |
22 |
8.5 |
8.5 |
8.6 |
8.2 |
Table 2. O2concentrations
nominal concentration (mg/L) |
measured concentration (mg/L) 0 h |
measured concentration (mg/L) 24 h old |
measured concentration (mg/L) 24 h new |
measured concentration (mg/L) 48 h old |
geometric mean (mg/L) |
0 |
< LOQ |
< LOQ |
< LOQ |
< LOQ |
< LOQ |
1.0 |
1.28 |
1.15 |
1.20 |
1.12 |
1.18 |
2.2 |
2.59 |
2.24 |
2.61 |
2.11 |
2.38 |
4.6 |
5.18 |
4.39 |
4.87 |
4.86 |
4.81 |
10 |
10.96 |
9.61 |
10.50 |
9.10 |
10.02 |
22 |
23.96 |
20.21 |
22.81 |
20.51 |
21.75 |
Table 3. measured concentrations
Description of key information
Key value is observed in a semi-static study exposing Daphnia magna for 48 hours to 2, 2'-azobis(2-methylpropionamidine) dihydrochloride. The test solution was changed after 24 hours to maintain the concentration above 80 % of nominal concentration.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- EC50
- Effect concentration:
- 3.5 mg/L
Additional information
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