2-methylbut-3-en-2-ol

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline with acceptable restrictions

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

1972

Report date:

1972

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 405 (Acute Eye Irritation / Corrosion)

Deviations:

yes

Remarks:

50 μl instead of 100 μl test substance instilled; two animals tested; missing 72 h reading

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

2-methylbut-3-en-2-ol

EC Number:

204-068-4

EC Name:

2-methylbut-3-en-2-ol

Cas Number:

115-18-4

Molecular formula:

C5H10O

IUPAC Name:

2-methylbut-3-en-2-ol

Details on test material:

- Name of test material (as cited in study report): 2-methylbut-3-en-2-ol
- Analytical purity: 98.5-99%
- Physical state: clear liquid

Test animals / tissue source

Species:

rabbit

Strain:

Vienna White

Details on test animals or tissues and environmental conditions:

TEST ANIMALS
- Source: Gaukler
- Weight at study initiation: 3.15 kg (male), 3.39 kg (female)

Test system

Vehicle:

unchanged (no vehicle)

Controls:

other: untreated eye of same animal

Amount / concentration applied:

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.05 mL
- Concentration (if solution): 100%

Duration of treatment / exposure:

single application

Observation period (in vivo):

8 d

Number of animals or in vitro replicates:

2

Details on study design:

REMOVAL OF TEST SUBSTANCE
- Washing (if done): no


SCORING SYSTEM:

The original BASF grading was converted into the numerical grading according to the OECD Draize system:

TOOL USED TO ASSESS SCORE: fluorescein

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

cornea opacity score

Basis:

mean

Time point:

other: 24, 48 h

Score:

2.3

Max. score:

4

Reversibility:

fully reversible within: 8 d

Results 2

Irritation parameter:

iris score

Basis:

mean

Time point:

other: 24, 48 h

Score:

0

Max. score:

2

Reversibility:

other: no effects

Results 3

Irritation parameter:

conjunctivae score

Basis:

mean

Time point:

other: 24, 48 h

Score:

1.5

Max. score:

3

Reversibility:

fully reversible within: 8 d

Results 4

Irritation parameter:

chemosis score

Basis:

mean

Time point:

other: 24, 48 h

Score:

1.75

Max. score:

4

Reversibility:

fully reversible within: 7 d

Any other information on results incl. tables

Results:

Animal 1: male, 3.15 kg prior to study and 3.32 kg at the end of study

Animal 2: female, 3.39 kg prior to study and 3.4 kg at the end of study

Readings	Animal	Corneal opacity	Iris	conjunctiva
				Erythema	Chemosis	Additional findings
24 h	1	2	0	2	2	m, o
	2	2	0	2	2	m, o
48 h	1	3	0	1	2	o, b
	2	2	0	1	1	b
5 d	1	1	0	1	1	b
	2	1	0	1	0	b
7 d	1	0	0	1	0
	2	1	0	0	0
8 d	1	0	0	0	0
	2	0	0	0	0
Mean 24 - 72 h	1	2.5	0.0	1.5	2.0
	2	2.0	0.0	1.5	1.5
Mean		2.3	0.0	1.5	1.75

m: myosis

o: overlay

b: bloody