Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 204-909-5 | CAS number: 128-80-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: dermal
Administrative data
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- secondary literature
- Justification for type of information:
- Data is from secondary source.
Data source
Reference
- Reference Type:
- other: seconary source
- Title:
- DERMAL, EYE, AND ORAL TOXICOLOGICAL EVALUATIONS
- Author:
- Indu A. Muni, Elliot B. Gordon,Jane B. Goodband
- Year:
- 1 986
- Bibliographic source:
- U.S. Army Medical Bioengineering Research and Development Laboratory
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: As mentioned below
- Principles of method if other than guideline:
- Acute dermal toxicity study was conducted by using the given test chemical.
- GLP compliance:
- not specified
- Test type:
- other: not specified
- Limit test:
- no
Test material
- Reference substance name:
- 1,4-bis(p-tolylamino)anthraquinone
- EC Number:
- 204-909-5
- EC Name:
- 1,4-bis(p-tolylamino)anthraquinone
- Cas Number:
- 128-80-3
- Molecular formula:
- C28H22N2O2
- IUPAC Name:
- 1,4-bis[(4-methylphenyl)amino]-9,10-anthraquinone
- Test material form:
- solid
- Details on test material:
- - Name of test material (IUPAC name): 1,4-Bis(p-tolylamino)anthraquinone
- Common name: C.I. Solvent Green 3
- Molecular formula: C28H22N2O2
- Molecular weight: 418.4938 g/mol
- Smiles notation: c12c(c(Nc3ccc(cc3)C)ccc1Nc1ccc(cc1)C)C(=O)c1c(C2=O)cccc1
- InChl: 1S/C28H22N2O2/c1-17-7-11-19(12-8-17)29-23-15-16-24(30-20-13-9-18(2)10-14-20)26-25(23)27(31)21-5-3-4-6-22(21)28(26)32/h3-16,29-30H,1-2H3
- Substance type: Organic
- Physical State: Solid
Constituent 1
Test animals
- Species:
- rabbit
- Strain:
- New Zealand White
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Rabbits were received from Pine Acres Rabbi try, West Brattleboro, Vermont
- Age at study initiation: These animals were 8-15 weeks old when obtained.
- Housing: The rabbits were individually housed in stainless steel cages,
- Diet (e.g. ad libitum): Fed Charles River Rabbit Formula (Agway) ad libitum and
- Water (e.g. ad libitum): provided untreated municipal water via water bottles
- Acclimation period: They were quarantined for two weeks
ENVIRONMENTAL CONDITIONS
- Temperature (°C): Animals were maintained at a temperature range of 67°F to 72°F
- Humidity (%): relative humidity range of 35% to 65%
- Air changes (per hr): Rooms are provided 12-16 air changes per hour
- Photoperiod (hrs dark / hrs light): Fluorescent lighting was controlled to provide a 12-hour
light cycle (1 AM to 7 PM).
Administration / exposure
- Type of coverage:
- occlusive
- Vehicle:
- other: 0.9% sodium chloride
- Details on dermal exposure:
- TEST SITE
- Area of exposure: The hair was carefully removed from the back and sides of each animal prior to testing. Oster animal clippers were used for this procedure.
- % coverage: 6 in. x 6 in
- Type of wrap if used: The trunk of the animal was wrapped in plastic wrap and then stockinette to prevent removal of the patches by the animal.
REMOVAL OF TEST SUBSTANCE
- Washing (if done): the wrappings and patches were removed.
- Time after start of exposure:24 hour - Duration of exposure:
- 24 hour
- Doses:
- 2000 mg/kg bw
- No. of animals per sex per dose:
- five male and five female
- Control animals:
- not specified
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: All animals were observed 3 times on the day of dosing and twice daily thereafter for signs of toxicity. Body weights were determined twice weekly.
- Necropsy of survivors performed: yes, necropsies were performed on animals dying intercurrently, as well as those surviving treatment and sacrificed on day 14.
- Other examinations performed: Histopathological examination of treated and untreated skin application sites was performed on animals dying during the test period and on 2 animals per sex necropsied at the end of the test period. - Statistics:
- No data
Results and discussion
- Preliminary study:
- No data
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 2 000 mg/kg bw
- Based on:
- test mat.
- Mortality:
- No mortality was observed at 2000 mg/kg bw during the 14 day observation period.
- Clinical signs:
- other: The only overt sign of toxicity observed during the study was mild diarrhea displayed by female rabbit 8850 in the afternoon of day 1. This mild diarrhea subsided completely by the morning of day 2.
- Gross pathology:
- There were no gross visible lesions detected upon necropsy of all 10 rabbits at the termination of the study.
- Other findings:
- No data
Applicant's summary and conclusion
- Interpretation of results:
- other: Not classified
- Conclusions:
- The acute dermal LD50 value was considered to be >2000 mg/kg bw, when 10 male and female New Zealand White rabbits were treated with the given test chemical by dermal application.
- Executive summary:
The acute dermal toxicity study was conducted by using the given test chemical in 10 male and female New Zealand White rabbits at the dose concentration of 2000 mg/kg bw. The hair was carefully removed from the back and sides of each animal prior to testing. Oster animal clippers were used for this procedure. The powdered test material for the single dose trials was applied uniformly to a 6 in. x 6 in. pad at a dose of 2000 mg/kg, moistened with 0.9% sodium chloride, and secured with surgical tape to the previously clipped and abraded test site. The trunk of the animal was wrapped in plastic wrap and then stockinette to prevent removal of the patches by the animal. After a 24 hour exposure period, the wrappings and patches were removed. All animals were observed 3 times on the day of dosing and twice daily thereafter for signs of toxicity. Body weights were determined twice weekly, and necropsies were performed on animals dying intercurrently, as well as those surviving treatment and sacrificed on day 14. Histopathological examination of treated and untreated skin application sites was performed on animals dying during the test period and on 2 animals per sex necropsied at the end of the test period. No mortality was observed at 2000 mg/kg bw during the 14 day observation period. The only overt sign of toxicity observed during the study was mild diarrhea displayed by female rabbit 8850 in the afternoon of day 1. This mild diarrhea subsided completely by the morning of day 2. The body weights of 3 male and 4 female rabbits either remained constant or increased during the study. Male rabbit 8828 lost approximately 100 grams between days 0 and 3, but regained weight by day 7. Female rabbit 8861 and male rabbit 8810 decreased in weight by 200 grams between days 10 and 14. There were no gross visible lesions detected upon necropsy of all 10 rabbits at the termination of the study.
Hence, the acute dermal LD50 value was considered to be >2000 mg/kg bw, when 10 male and female New Zealand White rabbits were treated with the given test chemical by dermal application.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.