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EC number: 695-187-4 | CAS number: 166524-75-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 30 June 2000 to 27 July 2000
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- Study conducted in compliance with agreed protocols, with no or minor deviations from standard test guidelines and/or minor methodological deficiencies, which do not affect the quality of the relevant results. The study report was conclusive, done to a valid guideline and the study was conducted under GLP conditions.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 001
- Report date:
- 2001
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.13/14 (Mutagenicity - Reverse Mutation Test Using Bacteria)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 870.5100 - Bacterial Reverse Mutation Test (August 1998)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: MAFF (1988)
- Deviations:
- no
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 5-ethoxy-2-({5-ethoxy-7-fluoro-[1,2,4]triazolo[1,5-c]pyrimidin-2-yl}disulfanyl)-7-fluoro-[1,2,4]triazolo[1,5-c]pyrimidine
- EC Number:
- 695-187-4
- Cas Number:
- 166524-75-0
- Molecular formula:
- C14H12F2N8O2S2
- IUPAC Name:
- 5-ethoxy-2-({5-ethoxy-7-fluoro-[1,2,4]triazolo[1,5-c]pyrimidin-2-yl}disulfanyl)-7-fluoro-[1,2,4]triazolo[1,5-c]pyrimidine
- Test material form:
- solid: particulate/powder
- Remarks:
- migrated information: powder
- Details on test material:
- - Name of test material (as cited in study report): 2,2'-dithiobis (5-ethoxy-7-fluoro [1,2,4] triazolo [1,5-c] pyrimidine) (DEDS)
- Appearance: light yellow powder
- Storage conditions: room temperature
Constituent 1
Method
- Target gene:
- Salmonella typhimurium: histidine operon
Escherichia coli: tryptophan operon
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Additional strain / cell type characteristics:
- other: The tester strains also contained the rfa wall mutation and the uvrB gene deletion. Strains TA98 and TA100 also contained the R-factor plasmid (pKM101).
- Species / strain / cell type:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 mix
- Test concentrations with justification for top dose:
- - Dose Range-finding assay: 0 (vehicle control), 6.67, 10.0, 33.3, 66.7, 100, 333, 667, 1000, 3330 and 5000 µg/plate
- Initial mutagenicity assay with S. typhimurium: 0 (vehicle control), 10.0, 33.3, 100, 333, 1000 and 5000 µg/plate (with S9 mix); 0 (vehicle control), 3.33, 10.0, 33.3, 100, 333, 1000 and 5000 µg/plate (without S9 mix)
- Initial mutagenicity assay with E. coli: 0 (vehicle control), 10.0, 33.3, 100, 333, 1000 and 5000 µg/plate (with and without S9 mix)
- Confirmatory mutagenicity assay with S. typhimurium: 0 (vehicle control), 3.33, 10.0, 33.3, 100, 333, 1000 and 5000 µg/plate (with and without S9 mix)
- Confirmatory mutagenicity assay with E. coli: 0 (vehicle control), 10.0, 33.3, 100, 333, 1000 and 5000 µg/plate (with and without S9 mix) - Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO
- Justification for choice of solvent/vehicle: the test material formed non-homogeneous suspensions in water (99.8 and 49.9 mg/mL), dimethylformamide (100.4 mg/mL), ethanol (100.2 mg/mL), acetone (106.2 mg/mL) and in dimethylsulfoxide (99.8 mg/mL). With dimethylsulfoxide, the test material formed a homogeneous suspension at a concentration of 49.9 mg/mL with sonication. For this reason, dimethylsulfoxide was selected as the vehicle in this study.
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 4-nitroquinoline-N-oxide
- 2-nitrofluorene
- sodium azide
- benzo(a)pyrene
- other: 2-aminoanthracene, ICR-191
- Details on test system and experimental conditions:
- DOSE RANGEFINDING STUDY
Performed with tester strains TA100 and WP2uvrA both with and without S9 mix. Ten doses of test material were tested at one plate per dose. The test material was checked for cytotoxicity up to a maximum concentration of 5 mg per plate.
METHOD OF APPLICATION: preincubation
The test material or vehicle control (100 µL), tester strain (100 µL) and S9 mix (0.5 mL) or 0.1M phosphate buffer (0.5 mL) were preincubated for 20 ± 2 minutes at 37 ± 2 °C prior to the addition of molten selective overlay agar (2 mL). The agar and the preincubation reaction mixture were mixed and then overlaid onto the surface of 25 mL of minimal bottom agar contained in a petri dish. After the overlay had solidified, the plates were inverted and incubated at 37 ± 2 °C for 52 ± 4 hours. Positive control materials were plated using a 50 µL aliquot of control material dose in a similar manner to the test material. Following incubation, revertant colonies were counted. All doses of test material, vehicle controls and positive controls were plated in triplicate.
DETERMINATION OF CYTOTOXICITY
The condition of the background lawn was evaluated for evidence of cytotoxicity and test material precipitate. - Evaluation criteria:
- CRITERIA FOR A VALID ASSAY
The reverse mutation assay may be considered valid if the following criteria are met:
- All bacterial strains must have demonstrated the required characteristics as determined by their respective strain checks.
- All tester strain cultures should exhibit a characteristic number of spontaneous revertants per plate in the vehicle and untreated controls.
- The density of all tester strain cultures should be greater than or equal to 0.5 x 10⁹ bacteria per mL and/or should have reached a target level of turbidity demonstrated to produce cultures with a density greater than or equal to 0.5 x 10⁹ bacteria per mL.
- Diagnostic mutagens (positive control chemicals) must be included to demonstrate both the intrinsic sensitivity of the tester strains to mutagen exposure and the integrity of the S9-mix. All of the positive control chemicals used in the study should induce marked increases (at least 3-fold) in the frequency of revertant colonies, both with or without metabolic activation.
- There should be a minimum of three non-toxic test material dose levels.
CRITERIA FOR A POSITIVE RESPONSE
For a test material to be considered positive, it has to produce at least a 3-fold (TA98, TA1535, TA1537, and WP2uvrA) or 2-fold (TA100) dose related and reproducible increase in the mean revertants per plate of at least one tester strain over the mean revertants per plate of the appropriate vehicle control.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity, but tested up to precipitating concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity, but tested up to precipitating concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Additional information on results:
- RANGE-FINDING/SCREENING STUDIES: Indications of cytotoxicity were observed with tester strain TA100 at 66.7 to 1000 µg/plate in the absence of S9 mix as evidenced by a thinning of the bacterial background lawn. No cytotoxicity was observed with tester strain TA100 in the presence of S9 mix or with WP2uvrA in either the presence or absence of S9 mix. The background lawns above 1000 µg/plate were obscured by precipitate.
DEFINITIVE MUTAGENICITY ASSAY: In the initial mutagenicity assay, and in the confirmatory assay, all data were acceptable and no positive increases in the mean number of revertants per plate were observed with any of the tester strains in either the presence or absence of S9 mix. - Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Table 1: Summary of Initial Mutagenicity Assay Results
Metabolic activation |
Treatment |
Dose (µg/plate) |
Mean revertants/plate |
Background lawn* |
Mean revertants/plate |
Background lawn* |
|||
TA98 |
TA100 |
TA1535 |
TA1537 |
WP2uvrA |
|||||
with S9 mix |
Vehicle control |
0 |
26 |
92 |
11 |
6 |
1 |
11 |
1 |
Test material |
10.0 |
25 |
106 |
11 |
7 |
1 |
14 |
1 |
|
33.3 |
28 |
103 |
10 |
6 |
1 |
13 |
1 |
||
100 |
24 |
103 |
9 |
7 |
1/2x |
12 |
1 |
||
333 |
26 |
94 |
11 |
6 |
1sp/2spx |
13 |
1sp |
||
1000 |
24 |
87 |
12 |
7 |
1sp/2spx |
12 |
1sp |
||
5000 |
21 |
105 |
8 |
4 |
6sp |
9 |
6sp |
||
Positive control |
** |
378 |
1044 |
135 |
170 |
1 |
202 |
1 |
|
without S9 mix |
Vehicle control |
0 |
15 |
96 |
10 |
7 |
1 |
12 |
1 |
Test material |
3.33 |
15 |
88 |
14 |
6 |
1 |
- | - | |
10.0 |
13 |
100 |
15 |
3 |
1 |
14 |
1 |
||
33.3 |
15 |
96 |
13 |
4 |
1 |
14 |
1 |
||
100 |
17 |
98 |
14 |
4 |
1/2+ |
10 |
1 |
||
333 |
16 |
86 |
10 |
5 |
2sp |
15 |
1sp |
||
1000 |
16 |
87 |
9 |
3 |
2sp |
10 |
1sp |
||
5000 |
9 |
93 |
9 |
8 |
6sp |
16 |
6sp |
||
Positive control |
*** |
272 |
579 |
575 |
2010 |
1 |
499 |
1 |
** TA98: benzo[a]pyrene (2.5 µg/plate); TA100: 2 -aminoanthracene (2.5 µg/plate); TA1535: 2 -aminoanthracene (2.5 µg/plate); TA1537: 2 -aminoanthracene (2.5 µg/plate); WP2uvrA: 2 -aminoanthracene (25.0 µg/plate)
*** TA98: 2 -nitrofluorene (1.0 µg/plate); TA100: sodium azide (2.0 µg/plate); TA1535: sodium azide (2.0 µg/plate); TA1537: ICR-191 (2.0 µg/plate); WP2uvrA: 4 -nitroquinoline-N-oxide (0.4 µg/plate)
* Background lawn evaluation
1 = normal; 2 = slightly reduced; 3 = moderately reduced; 4 = extremely reduced; 5 = absent; 6 = obscured by precipitate; sp = slight precipitate
x = The first entry is the lawn evaluation for tester strains TA100 and TA1535. The second entry is the lawn evaluation for tester strains TA98 and TA1537
+ = The first entry is the lawn evaluation for tester strains TA1535 and TA1537. The second entry is the lawn evaluation for tester strains TA98 and TA100
Table 2: Summary of Confirmatory Mutagenicity Assay Results
Metabolic activation |
Treatment |
Dose (µg/plate) |
Mean revertants/plate |
Background lawn* |
Mean revertants/plate |
Background lawn* |
|||
TA98 |
TA100 |
TA1535 |
TA1537 |
WP2uvrA |
|||||
with S9 mix |
Vehicle control |
0 |
24 |
65 |
7 |
12 |
1 |
19 |
1 |
Test material |
3.33 |
26 |
88 |
12 |
11 |
1 |
- | - | |
10.0 |
25 |
78 |
14 |
10 |
1 |
19 |
1 |
||
33.3 |
24 |
83 |
10 |
10 |
1 |
14 |
1 |
||
100 |
29 |
91 |
14 |
9 |
1 |
15 |
1 |
||
333 |
20 |
83 |
9 |
7 |
1sp |
10 |
1sp |
||
1000 |
22 |
97 |
10 |
8 |
1sp |
12 |
1sp |
||
5000 |
15 |
82 |
12 |
9 |
6sp |
15 |
6sp |
||
Positive control |
** |
215 |
873 |
127 |
177 |
1 |
286 |
1 |
|
without S9 mix |
Vehicle control |
0 |
15 |
86 |
13 |
5 |
1 |
12 |
1 |
Test material |
3.33 |
15 |
105 |
11 |
8 |
1 |
- | - | |
10.0 |
13 |
89 |
16 |
6 |
1 |
17 |
1 |
||
33.3 |
12 |
83 |
11 |
9 |
1 |
13 |
1 |
||
100 |
14 |
92 |
12 |
7 |
1/2x |
17 |
1 |
||
333 |
18 |
88 |
12 |
9 |
1sp/2spx |
13 |
1sp |
||
1000 |
17 |
81 |
13 |
6 |
1sp/2spx |
18 |
1sp |
||
5000 |
16 |
81 |
14 |
3 |
6sp |
11 |
6sp |
||
Positive control |
*** |
285 |
601 |
564 |
1875 |
1 |
329 |
1 |
** TA98: benzo[a]pyrene (2.5 µg/plate); TA100: 2 -aminoanthracene (2.5 µg/plate); TA1535: 2 -aminoanthracene (2.5 µg/plate); TA1537: 2 -aminoanthracene (2.5 µg/plate); WP2uvrA: 2 -aminoanthracene (25.0 µg/plate)
*** TA98: 2 -nitrofluorene (1.0 µg/plate); TA100: sodium azide (2.0 µg/plate); TA1535: sodium azide (2.0 µg/plate); TA1537: ICR-191 (2.0 µg/plate); WP2uvrA: 4 -nitroquinoline-N-oxide (0.4 µg/plate)
* Background lawn evaluation
1 = normal; 2 = slightly reduced; 3 = moderately reduced; 4 = extremely reduced; 5 = absent; 6 = obscured by precipitate; sp = slight precipitate
x = The first entry is the lawn evaluation for tester strains TA98, TA1535 and TA1537. The second entry is the lawn evaluation for tester strains TA100
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative with and without metabolic activation
Under the conditions of the study, the test material did not cause a positive increase in the mean number of revertants per plate with any of the tester strains either in the presence or absence of metabolic activation. It can therefore be considered non-mutagenic under the conditions of this assay. - Executive summary:
The mutagenic potential of the test material was investigated in a study which was conducted under GLP conditions and in accordance with the standardised guidelines OECD 471, EU Method B.13/14, EPA OPPTS 870.5100 and Japanese MAFF.
The tester strains used in the initial mutagenicity assay were Salmonella typhimurium strains TA98, TA100, TA1535, TA1537 and Escherichia coli strain WP2uvrA. The assay was conducted with a minimum of six dose levels of test material (up to 5000 µg/plate) both in the presence and absence of S9 mix along with concurrent vehicle and positive controls using three plates per dose.
The doses initially tested in the mutagenicity assay were selected based on the results of a dose rangefinding study using tester strains TA100 and WP2uvrA and ten doses of test material ranging from 6.67 to 5000 µg/plate, with one plate per dose, both in the presence and absence of S9 mix.
The results of the initial mutagenicity assay were confirmed in an independent confirmatory assay.In the initial mutagenicity assay, and in the confirmatory assay, all data were acceptable and no positive increases in the mean number of revertants per plate were observed with any of the tester strains in either the presence or absence of S9 mix. Therefore, under the conditions of the study, the test material did not cause a positive increase in the mean number of revertants per plate with any of the tester strains either in the presence or absence of metabolic activation. It can therefore be considered non-mutagenic under the conditions of this assay.
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