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EC number: 203-703-2 | CAS number: 109-77-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
Study was conducted in compliance with the GLP-standards as set forth in title 40, of U.S. Code of Federal Regulation, part 160, with EPA, TOSCA GLP Regulation 40 CFR part 792.
25 male and 25 female rats (28-day study) and 75 male and 75 female rats (90-day study) of the Crl: CD (SD) BR strain were used.
Test article was formulated in water and administered orally by gavage, once daily. A constant dose volume of 10mg/kg/day (group 5) was used for 14 days.
90-day study, treatment at 0.4 mg/kg/day was not associated with any toxicologically significant changes and can therefore be considered as the no-effect level for this compound in the rat.
NOEL 0.4 mg/kg/bw/day.
Key value for chemical safety assessment
Repeated dose toxicity: via oral route - systemic effects
Endpoint conclusion
- Dose descriptor:
- NOAEL
- 0.4 mg/kg bw/day
Additional information
The objective of the study was to determine the oral toxicity of Malononitrile in the rat following administration for 28 days and to select dose levels for a subsequent 90 day toxicity study.
The majority of high dose (4 mg/kg/day and 8 mg/kg/day from beginning of week 3) males had AST (GOT) levels above those of the controls, and the high dose females had urea levels outside the control range. The mean absolute and relative liver weights of the high dose males were greater than those of the controls. Microscopically, the high dose males had centrilobular hypertrophy in the liver and hyaline droplets in the kidney. No effects at dose level 0.04 mg/kg/day and 0.4 mg/kg/day after 28 days treatment.
To demonstrate a clear toxic effect, 10 mg/kg/bw/day is considered to be a suitable high dose level for the subsequent 90 -day study.
90 -day study, treatment at a dose level of 10 mg/kg/day brought a increase in liver weight which was associated with hepatocyte hypertrophy and vacuolation on histopathological examination. Clinical pathology changes in alkaline phospatase and cholesterol levels my be related to these findings. The clinical pathology and histopathology changes were completely reversible after a four week period without treatment, although there remained a small difference in the weight of the liver. The increased plasma urea levels in animals treated at 10 mg/kg/day and females treated at 2 mg/kg/day are therefore of unknown origin and do not, in isolation, indicate a clear effect on the kidney.
Treatment at 0.4 mg/kg/day was not associated with any toxicologically significant changes and can therefore be considered as the no-effect level for this compound in the rat.
Justification for classification or non-classification
According to the results of the 90 -day study there is no reason for classification.
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