Registration Dossier
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EC number: 942-438-3 | CAS number: -
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
Description of key information
ErL50 (algae; growth rate) = > 7.50 mg/L ; WAF loading 72hour-freshwater, OECD TG 201, Dr.U.NOACK-Laboratorien 2015
ErL10 (algae; growth rate)= 6.82 mg/L (C.I. 4.39 - > 7.50 ) ; WAF loading 72 hour-freshwater, OECD TG 201, Dr.U.NOACK-Laboratorien 2015
NOELR (algae; growth rate) = 4.20 mg/L ; WAF loading, 72 hour-freshwater, OECD TG 201, Dr.U.NOACK-Laboratorien 2015
Key value for chemical safety assessment
Additional information
The algal growth inhibition to Pseudokirchneriella subcapitata, was carried out according to OECD 201 Freshwater Alga and Cyanobacteria, Growth Inhibition Test and EU Method C.3 guidelines. The aim of the study was to assess the effects on growth rate and yield over a period of 72 hours. The study was conducted under static conditions with an initial cell density of 6153 cells/mL. Five Water accommodated fractions (WAF) were tested in a geometrical series with a dilution factor of 1.33 (nominal): 2.4, 3.2, 4.2, 5.6, 7.5 mg/L. Three replicates were tested for each test item concentration and six replicates for the control. The environmental conditions were within the acceptable limits. The concentrations of the test item were analytically verified via GC-MS at the start of the exposure and after 24, 48 and 72 hours, respectively, in all test levels and the control. These analytical data were used as supporting information to monitor the stability of the three constituents of the test item in the test system during the study. The measured concentrations of the test item were in the range of 48 to 84 % of the 0 hour measured concentrations after 72 hours. All effect values given are based on nominal test item concentrations of the test item. Per definition of the WAF, all terms related to concentration levels have to be given as loading levels because partly dissolved compounds and mixtures cannot be related to concentrations. The validity criteria of the test guideline were fulfilled. The EL50 for growth rate reduction (72h-ErL50) based on nominal loading in a WAF was > 7.50 mg/L. The corresponding NOELR was 4.20 mg/L..
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