Isopropenyl methyl ether

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: study performed according to OECD and GLP guidelines

Cross-reference

Reason / purpose for cross-reference:

reference to same study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2000

Report date:

2001

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

Principles of method if other than guideline:

-

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Isopropenyl methyl ether

EC Number:

204-125-3

EC Name:

Isopropenyl methyl ether

Cas Number:

116-11-0

Molecular formula:

C4H8O

IUPAC Name:

2-methoxyprop-1-ene

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Cyprinus carpio

Study design

Test type:

flow-through

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

>= 100 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Any other information on results incl. tables

Sublethal observations / clinical signs:

-

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Under the conditions of the present flow-through test ISOPROPENYL METHYL ETHER induced
no lethal effects in carp at an exposure concentration of 109 mg/l ± 20%.
The 96h-LC50 of ISOPROPENYL M ETHYL ETHER for fish exceeded the regulatory limit
concentration of 100 mg/l

Executive summary:

The study procedures described in this report were based on the EEC directive 92169, Part C.1 . "Acute toxicity for fish"; and the OECD guideline No. 203: "Fish Acute Toxicity Test", Adopted 17 July, 1992. The batch of ISOPROPENYL METHYL ETHER tested was a clear colourless liquid with a purity of 97.6 %. The test substance was completely soluble in test medium at the concentrations tested. The project started with a static range-finding test exposing carp to test concentrations of 0.1, 1.0, 10 and 100 mg/l. No visible effects were observed during the test in any of the concentrations tested. No results are reported for the samples from the range-finding test owing to problems with the chromatographic system and the volatility of the test substance. Testing in a flow-through system was considered to be the best alternative. The experimental design was optimised by introducing continuous dosing of the test solution in a flow-through system. The target concentration for the final test was 100 mg/l. Daily 50 ml of the original batch of ISOPROPENYL METHYL ETHER were dosed at a rate of 1.59 ml/h. In a mixing flask, the dosed volume and tap water (flow: ca. 12 Uh) were mixed under continuous stirring. Seven carp were exposed per test solution. Samples for analytical confirmation of actual exposure concentrations were taken before the start of exposure, after 48 hours and at the end of the test. The analytical program confirmed that the actual concentration had been maintained at the target concentration, i.e. 109 mg/l ± 20%. All test conditions (pH, dissolved oxygen and temperature) remained within the ranges prescribed by the protocol. No mortality was observed in the control group. Exposure to the ISOPROPENYL METHYL ETHER concentration of 100 mg/l induced no mortality offish. Visible effects were limited to hypoactivity and swimming at the surface of the test soluti'ons. In conclusion, ISOPROPENYL METHYL ETHER induced no lethal effects in carp at an exposure concentration of 109 mg/l ± 20%. The 96h-LC50 of ISOPROPENYL M ETHYL ETHER for fish exceeded the regulatory limit concentration of 100 mg/l.