Methyl [(dimethoxyphosphinothioyl)thio]acetate

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From 01-10-1992 to 28-10-1992

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1992

Report date:

1992

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

Deviations:

no

GLP compliance:

yes

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Methyl [(dimethoxyphosphinothioyl)thio]acetate

EC Number:

212-058-6

EC Name:

Methyl [(dimethoxyphosphinothioyl)thio]acetate

Cas Number:

757-86-8

Molecular formula:

C5H11O4PS2

IUPAC Name:

methyl 2-{[dimethoxy(sulfanylidene)-λ⁵-phosphanyl]sulfanyl}acetate

Details on test material:

- Name of test material (as cited in study report): MPEM
- Substance type: Intermediate
- Physical state: Liquid
- Purity test date:
- Lot/batch No.: 20825

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Møllegaard Breeding Centre Ltd, Ejby, DK-4623 Ll. Skensved
- Age at study initiation: 7-8 weeks
- Weight at study initiation: 201-249 g
- Fasting period before study: no
- Housing: Macrolone cages type III, 2 or 3 to a cage, males and females separated. The bedding was pinewood sawdust "Linocel Granulat" from Compagny Altromin GmbH D-4937 Lage, Lippe, Germany
- Diet : "Altromin 1314" from Compagny Altromin GmbH D-4937 Lage, Lippe, Germany
- Water : ad libitum, acidified with hydrochloric acid to pH 2,5.
- Acclimation period: 5 days

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 21 ± 3
- Humidity (%):55, ± 15
- Air changes (per hr): 10 times
- Photoperiod (hrs dark / hrs light): 12/12

Administration / exposure

Type of coverage:

occlusive

Vehicle:

unchanged (no vehicle)

Details on dermal exposure:

TEST SITE
- Area of exposure:
- % coverage: -
- Type of wrap if used: 4 layer gauze, fixed with Scanpor tape

REMOVAL OF TEST SUBSTANCE
- Washing (if done): luke warm water and soap
- Time after start of exposure: 24 hours after

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 4000 mg/kg b.w.
- Concentration (if solution): pure

Duration of exposure:

24 hours

Doses:

4000 mg / kg body weight

No. of animals per sex per dose:

5

Control animals:

not required

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Observations: 3 times the first day, thereafter daily
Weighing: on day 0, 7 and 14
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight,organ weights, histopathology, other:

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 4 000 mg/kg bw

Based on:

test mat.

Mortality:

None

Clinical signs:

other: Piloerection on day of treatment observed, from day 1 and onwards no symptoms

Gross pathology:

None

Other findings:

Other than piloerection on day of exposure, none

Applicant's summary and conclusion

Interpretation of results:

not classified

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

Since no rats died from the treatment, the LD50 for MPEM must be above 4000 mg/kg b.w.